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Spontaneous Miscarriage strongly associated with 2 vitamin D genes – March 2020

Altered decidual and placental catabolism of vitamin D may contribute to the aetiology of spontaneous miscarriage

Placenta, Volume 92, March 2020, Pages 1-8, https://doi.org/10.1016/j.placenta.2020.01.013
lH.HouaJ.Y.ZhangaD.ChenaF.DengbA.N.MorsebX.QiucP.HebG.E.Lasha

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Placenta

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Decidua

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Highlights

  • Placental/decidual levels of vitamin D are decreased in spontaneous miscarriage.
  • CYP enzyme expression changes may be linked to hyperoxia.
  • VDR expression changes may be linked to infection.

Introduction
Vitamin D catabolizing enzymes, along with vitamin D receptor (VDR) and vitamin D binding protein (DBP) are expressed in the decidua and placenta during pregnancy and capable of synthesizing active vitamin D. Vitamin D plays roles in immunoregulation and trophoblast invasion, key features of a successful pregnancy. Epidemiological data suggests that vitamin D deficiency is associated with both spontaneous and recurrent miscarriage but few studies have investigated the expression of the key vitamin D catabolizing enzymes in miscarriage.

Methods
Placenta and decidua were collected after termination of apparently normal pregnancies (controls, n = 22) or spontaneous miscarriage (n = 20). Immunohistochemical staining, Western Blot and qRT-PCR were performed for CYP27B1, CYP24A1, CYP2R1, VDR and DBP (not qRT-PCR). HTR-8/SVneo cells were cultured in CoCL2 (hypoxic mimetic) or LPS (bacterial infection mimetic) for 24 h, RNA extracted and qRT-PCR performed for CYP27B1, CYP24A1, CYP2R1 and VDR.

Results
In spontaneous miscarriage, placental and decidual

  • expression of CYP27B1 was reduced, while
  • expression of CYP24A1, VDR and DBP was increased.

When a trophoblast cell line was treated with CoCL2 expression of CYP27B1 was increased and CYP24A1 was reduced, while LPS induced expression of VDR.

Discussion
This is the first report of altered utero-placental vitamin D catabolism in spontaneous miscarriage. It is becoming accepted that women who are undergoing assisted reproductive technologies should ensure they have sufficient vitamin D levels prior to pregnancy, these data support that all women should ensure they are vitamin D replete before planning to get pregnant.

Attached files

ID Name Comment Uploaded Size Downloads
13500 Decidua.jpg admin 15 Feb, 2020 12:52 21.22 Kb 119
13499 Placenta.jpg admin 15 Feb, 2020 12:51 24.66 Kb 89
13498 Placenta genes miscarriage.pdf PDF 2020 admin 15 Feb, 2020 12:43 1.49 Mb 29
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