Assessment of serum vitamin D level and seminal vitamin D receptor gene methylation in a sample of Egyptian men with idiopathic infertility
Andrologia . 2021 Jul 1;e14172. doi: 10.1111/and.14172
Tarek M Hussein 1, Nermeen Eldabah 2, Hadil A Zayed 1, Rasha M Genedy 1
Fertility and Sperm category contains the following summary
See also:
Overview Women and Vitamin D
Overview Pregnancy and vitamin D Fertility and Vitamin D – several articles
Endometriosis
Ensure a healthy pregnancy and baby - take Vitamin D before conception
IVF OR "IN VITRO FERTILIZATION" etc. in 18 VitaminDWiki titles
Vitamin D greatly improves Fertility
- Vitamin D is needed for human fertility – goal is 50 ng – Sept 2018
- In-vitro Fertilization costs at least 10,000 dollars, Vitamin D costs 5 dollars
- Women were 50X more likely to be fertile if just 1 ng higher level of vitamin D – Nov 2024
- Infertile patients 1.7X more-likely to become pregnant if take Vitamin D – meta-analysis Feb 2023
- Live birth 1.7 X more likely after IVF if good level of vitamin D – meta-analysis Aug 2020
- If diagnosed infertile, more likely to have live birth if Vitamin D fortification – Feb 2020
- Preconception vitamin D is great - every extra 10 ng associated with 10 percent more likely to have live birth – Aug 2018
- Women with more than minimum vitamin D were 3.4 X more likely to achieve pregnancy and 1.6 X more likely to have live births – June 2017
- Assisted Reproduction – 5 studies concluded vitamin D repletion helps – Review March 2015
- Pregnancy success increased 30 percent if sunny (or vitamin D) one month earlier – June 2015
- IVF 4X more successful for white women with lots of vitamin D – many studies
Increased male Vitamin D increases fertility
- Birth rates doubled with Vitamin D - 300,000 for infertile men – RCT Nov 2017
- Higher vitamin D results in 22% fewer abnormal sperm (Mendelian analysis) – May 2024
- Conception was 3.7X more likely if the male had a good level of Vitamin D – July 2022
- Far better sperm in fertility clinic if more than 30 ng of Vitamin D - June 2022
- Fertility (sperm) associated with vitamin D – meta-analysis Sept 2019
- Infertility - 71 percent of the time of BOTH partners had less than 20 ng of Vitamin D – Aug 2017
- Male fertility 4 X higher if high Vitamin D – Nov 2015
- Vitamin D somewhat assists reproduction – both the mother and the father – May 2014
Decreased Fertility if decreased Vitamin D Receptor
- Unexplained infertility 4X more likely if poor vitamin D receptor – Dec 2020
- Male Infertility is associated with poor Vitamin D Receptor – July 2021
Items in both categories Fertility/sperm and Vitamin D Receptor:
- In vitro fertilization and Vitamin D – many studies
- Infertility risk increased 2.5 X by poor Vitamin D Receptor – July 2023
- Vitamin D levels in cells, not blood, is important (IVF follicular fluid in this case) – Aug 2021
- Male Infertility is associated with poor Vitamin D Receptor – July 2021
- Poor male fertility is association with poor Vitamin D Receptor (less D to the cells) – July 2021
- Unexplained infertility 4X more likely if poor vitamin D receptor – Dec 2020
- PCOS (polycystic ovary syndrome) 4 times more likely if poor Vitamin D Receptor – Dec 2015
The risk of 44 diseases at least double with poor Vitamin D Receptor as of Oct 2019
Vitamin D Receptor activation can be increased by any of: Resveratrol, Omega-3, Magnesium, Zinc, Quercetin, non-daily Vit D, Curcumin, intense exercise, Ginger, Essential oils, etc Note: The founder of VitaminDWiki uses 10 of the 12 known VDR activators
Vitamin D deficiency has been linked with different health problems including male infertility. Its function is mediated by the vitamin D receptor, which acts as a transcription factor. Epigenetic mechanisms such as DNA methylation may affect the vitamin D receptor gene and result in gene silencing. The present study aimed to assess serum vitamin D level and seminal methylation of vitamin D receptor gene in idiopathic male infertility. Blood and semen samples were collected from 60 men with idiopathic infertility and 40 healthy fertile men. Vitamin D levels were detected using enzyme-linked fluorescent assay technique and methylation status was assessed by methylation-specific PCR. Results revealed that serum levels of 25OHD were significantly lower in patients compared to controls. Positive correlation was found between serum level of 25OHD and sperm concentration in patients group and progressive motility in total studied group. Methylation of vitamin D receptor gene was significantly higher in patients compared to control group. Negative correlation was found between methylation of vitamin D receptor gene and both sperm concentration and progressive motility in total studied group. Results of the present study suggest that vitamin D deficiency and vitamin D receptor gene methylation may be involved in aetiopathogenesis of idiopathic male infertility.
REFERENCES
- Abbasihormozi, S., Kouhkan, A., Alizadeh, A. R., Shahverdi, A. H., Nasr-Esfahani, M. H., Sadighi Gilani, M. A., Salman Yazdi, R., Matinibehzad, A., & Zolfaghari, Z. (2017). Association of vitamin D status with semen quality and reproductive hormones in Iranian subfertile men. Andrology, 5(1), 113-118. https://doi.org/10.1111/andr.12280
- Albaldawy, M. T., & Alsalami, A. S. (2017). Study of association among Vitamin D, testosterone and semen quality in fertile and Iraqi infertile men. Journal of Pharmaceutical Sciences and Research, 9(7), 1067.
- Alzoubi, A., Mahdi, H., Al Bashir, S., Halalsheh, O., Al Ebbini, M., Alzarir, M., Al-Ahmar, K., Alfaqih, M., & Al-Hadidi, A. H. (2017). Normalization of serum vitamin D improves semen motility parameters in patients with idiopathic male infertility. Acta Endocrinologica (Bucharest), 13(2), 180-187. https://doi.org/10.4183/aeb.2017.180
- Anagnostis, P., Karras, S., & Goulis, D. G. (2013). Vitamin D in human reproduction: A narrative review. International Journal of Clinical Practice, 67(3), 225-235. https://doi.org/10.1111/ijcp.12031
- Blomberg Jensen, M., Bjerrum, P. J., Jessen, T. E., Nielsen, J. E., Joensen, U. N., Olesen, I. A., Petersen, J. H., Juul, A., Dissing, S., & Jørgensen, N. (2011). Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa. Human Reproduction (Oxford, England), 26(6), 1307-1317. https://doi.org/10.1093/humrep/der059
- Blomberg Jensen, M., Gerner Lawaetz, J., Andersson, A. M., Petersen, J. H., Nordkap, L., Bang, A. K., Ekbom, P., Joensen, U. N., Praetorius, L., Lundstrøm, P., Boujida, V. H., Lanske, B., Juul, A., & Jørgensen, N. (2016). Vitamin D deficiency and low ionized calcium are linked with semen quality and sex steroid levels in infertile men. Human Reproduction (Oxford, England), 31(8), 1875-1885. https://doi.org/10.1093/humrep/dew152
- Boisen, I. M., Bøllehuus Hansen, L., Mortensen, L. J., Lanske, B., Juul, A., & Blomberg Jensen, M. (2017). Possible influence of vitamin D on male reproduction. The Journal of Steroid Biochemistry and Molecular Biology, 173, 215-222. https://doi.org/10.1016/j.jsbmb.2016.09.023
- Cavallini, G. (2006). Male idiopathic oligoasthenoteratozoospermia. Asian Journal of Andrology, 8(2), 143-157. https://doi.org/10.1111/j.1745-7262.2006.00123.x
- Ciccone, I. M., Costa, E. M., Pariz, J. R., Teixeira, T. A., Drevet, J. R., Gharagozloo, P., Aitken, R. J., & Hallak, J. (2021). Serum vitamin D content is associated with semen parameters and serum testosterone levels in men. Asian Journal of Andrology, 23(1), 52-58. https://doi.org/10.4103/aja.aja_9_20
- Cisneros, F. J. (2004). DNA methylation and male infertility. Frontiers in Bioscience: A Journal and Virtual Library, 9, 1189-1200. https://doi.org/10.2741/1332
- Fackler, M. J., McVeigh, M., Mehrotra, J., Blum, M. A., Lange, J., Lapides, A., Garrett, E., Argani, P., & Sukumar, S. (2004). Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer. Cancer Research, 64(13), 4442-4452. https://doi.org/10.1158/0008-5472.CAN-03-3341
- Goodrich, R., Johnson, G., & Krawetz, S. A. (2007). The preparation of human spermatozoal RNA for clinical analysis. Archives of Andrology, 53(3), 161-167. https://doi.org/10.1080/01485010701216526
- Holick, M. F., Binkley, N. C., Bischoff-Ferrari, H. A., Gordon, C. M., Hanley, D. A., Heaney, R. P., Murad, M. H., & Weaver, C. M. (2011). Evaluation, treatment, and prevention of vitamin D deficiency: An Endocrine Society clinical practice guideline. The Journal of Clinical Endocrinology and Metabolism, 96(7), 1911-1930. https://doi.org/10.1210/jc.2011-0385
- Hussain, F., Malik, A., Qureshi, M., Imran, M., Waquar, S., Shafique, H., Rana, Z., Thu, H., & Hussain, Z. (2018). Homeostatic relevance of vitamin D in maintaining male fertility in human: down-regulation of oxidative stress and upregulation of anti-oxidative defence and steroidal hormones. Asian Pacific Journal of Reproduction, 7(2), 56-61. https://doi.org/10.4103/2305-0500.228014
- Kumar, N., & Singh, A. K. (2015). Trends of male factor infertility, an important cause of infertility: A review of literature. Journal of Human Reproductive Sciences, 8(4), 191-196. https://doi.org/10.4103/0974-1208.170370
- Lerchbaum, E., & Obermayer-Pietsch, B. (2012). Vitamin D and fertility: A systematic review. European Journal of Endocrinology, 166(5), 765-778. https://doi.org/10.1530/EJE-11-0984
- Li, L. C., & Dahiya, R. (2002). MethPrimer: Designing primers for methylation PCRs. Bioinformatics (Oxford, England), 18(11), 1427-1431. https://doi.org/10.1093/bioinformatics/18.11.1427
- Ostermeier, G. C., Dix, D. J., Miller, D., Khatri, P., & Krawetz, S. A. (2002). Spermatozoal RNA profiles of normal fertile men. Lancet (London, England), 360(9335), 772-777. https://doi.org/10.1016/S0140-6736(02)09899-9
- Ramlau-Hansen, C. H., Moeller, U. K., Bonde, J. P., Olsen, J., & Thulstrup, A. M. (2011). Are serum levels of vitamin D associated with semen quality? Results from a cross-sectional study in young healthy men. Fertility and Sterility, 95(3), 1000-1004. https://doi.org/10.1016/j.fertnstert.2010.11.002
- Rehman, R., Lalani, S., Baig, M., Nizami, I., Rana, Z., & Gazzaz, Z. J. (2018). Association between vitamin D, reproductive hormones and sperm parameters in infertile male subjects. Frontiers in Endocrinology, 9, 607. https://doi.org/10.3389/fendo.2018.00607
- Saccone, D., Asani, F., & Bornman, L. (2015). Regulation of the vitamin D receptor gene by environment, genetics and epigenetics. Gene, 561(2), 171-180. https://doi.org/10.1016/j.gene.2015.02.024
- Vladoiu, S., Botezatu, A., Anton, G., Manda, D., Paun, D. L., Oros, S., Rosca, R., & Dinu Draganescu, D. (2017). The involvement of VDR promoter methylation, CDX-2 VDR polymorphism and vitamin D levels in male infertility. Acta Endocrinologica (Bucharest), 13(3), 294-301. https://doi.org/10.4183/aeb.2017.294
- World Health Organization (WHO). (2010). WHO laboratory manual for the Examination and processing of human semen. WHO.
- Yang, B., Sun, H., Wan, Y., Wang, H., Qin, W., Yang, L., Zhao, H., Yuan, J., & Yao, B. (2012). Associations between testosterone, bone mineral density, vitamin D and semen quality in fertile and infertile Chinese men. International Journal of Andrology, 35(6), 783-792. https://doi.org/10.1111/j.1365-2605.2012.01287.x
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