Table of contents
Vitamin D Alters Genes Involved in Follicular Development and Steroidogenesis in Human Cumulus Granulosa Cells.
J Clin Endocrinol Metab. 2014 Mar 14:jc20134161.
Merhi Z1, Doswell A, Krebs K, Cipolla M.
1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Vermont College of Medicine, Burlington, VT, USA.
Context: Vitamin D deficiency is common among reproductive-aged women and has a role in female reproduction.
Objective: This study evaluated the role of 1,25-dihydroxyvitamin D3 (vit D3) in ovarian follicular development and steroidogenesis by using a human granulosa cell (GC) model.
Design, setting and participants: 54 women who underwent IVF were enrolled. Intervention: Follicular fluid (FF), mural and cumulus GCs were collected from small (SF) and large follicles (LF). In separate experiments, primary cumulus GCs were cultured with or without vit D3 followed by RT-PCR for mRNA expression levels. The effect of recombinant anti-Mullerian hormone (AMH) on nuclear localization of phospho-Smad 1/5/8 was evaluated in the presence or absence of vit D3 by using immunofluorescence. 25 hydroxy-vitamin D (25 OH-D) levels in FF as well as cell culture media AMH, progesterone (P4), and estradiol (E2) concentrations were determined by ELISA and RIA.
Main outcome measures:
- 1) mRNA expression levels;
- 2) 3-βHSD enzyme activity;
- 3) FSH-induced aromatase mRNA and E2 production, and
- 4) nuclear localization of phospho-Smad 1/5/8.
Results: In multivariate analysis, 25 OH-D levels in FF negatively correlated with AMH and AMHR-II mRNA levels in cumulus GCs of SF.
Compared to women with replete 25 OH-D levels in FF, those with insufficient/deficient levels had a two-fold increase in AMHR-II mRNA levels in cumulus GCs of SF (p=0.02).
Treatment with vit D3 caused a decrease in AMHR-II and FSHR mRNA but an increase in 3-βHSD mRNA levels compared to control (p<0.05).
Vit D3 enhanced 3-βHSD enzyme activity as assessed by increasing P4 release;
- however, vit D3 did not affect FSH-induced aromatase mRNA and E2 production and
- it decreased the phosphorylation of Smad 1/5/8 and its nuclear localization.
Conclusion: These data suggest that vit D3 alters AMH signaling and steroidogenesis in human cumulus GCs, possibly reflecting a state of GC luteinization potentiation.
Female fertility for IVF needs at least 30ng of Vitamin D - no mention of vitamin D level of the sperm donor
Vitamin D and female fertility.
Curr Opin Obstet Gynecol. 2014 Apr 8
Lerchbaum E 1, Rabe T.
1a Division of Endocrinology and Metabolism, Department of Internal Medicine, Medical University of Graz, Graz, Austria bUniversity Women's Hospital, Heidelberg, Germany.
PURPOSE OF REVIEW:
Apart from the well known effects of vitamin D on maintaining calcium homeostasis and promoting bone mineralization, there is some evidence suggesting that vitamin D also modulates human reproductive processes. We will review the most interesting and relevant studies on vitamin D and female fertility published over the past year.
In the past year, several observational studies reported a better in-vitro fertilization outcome in women with sufficient vitamin D levels (≥30 ng/ml), which was mainly attributed to vitamin D effects on the endometrium. One randomized controlled trial found an increased endometrial thickness in women with polycystic ovary syndrome (PCOS) receiving vitamin D during intrauterine insemination cycles. Further, vitamin D supplementation had a beneficial effect on serum lipids in PCOS women. Vitamin D treatment improved endometriosis in a rat model and increased vitamin D intake was related to a decreased risk of incident endometriosis. Vitamin D was also favorably associated with primary dysmenorrhea, uterine leiomyoma, and ovarian reserve in late reproductive aged women.
In women undergoing in-vitro fertilization, a sufficient vitamin D level (≥30 ng/ml) should be obtained.
Vitamin D supplementation might improve metabolic parameters in women with PCOS.
A high vitamin D intake might be protective against endometriosis.
Fertility and Sperm category contains the following summary
Overview Women and Vitamin D
Overview Pregnancy and vitamin D Fertility and Vitamin D – several articles
Ensure a healthy pregnancy and baby - take Vitamin D before conception
Search VitaminDWiki for IVF OR "IN VITRO FERTILIZATION" 345 items as of Sept 2019
Search VitaminDWiki for "Assisted reproduction" 177 items as of Sept 2019
Search VitaminDWiki for "polycystic ovary syndrome" OR PCOS" Sept 2019
Search VitaminDWiki fore Testosterone 77 items just in webpages as of Sept 2020
Search VitaminDWiki for "erectile dysfunction" 120 items as of July 2018
Conception and vitamin D snapshot as of 2012
The TOP articles in Fertility and Sperm and Vitamin D are listed here:
- Vitamin D levels in cells, not blood, is important (follicular fluid in this case) – Aug 2021
- Conception within 6 months 3X more likely if high vitamin D – Oct 2019
- Premature ejaculation associated with low vitamin D – 2018, 2019
- Vitamin D is needed for human fertility – goal is 50 ng – Sept 2018
- Ensure a healthy pregnancy and baby - take Vitamin D before conception
- The earlier the better- preconception vitamin D - June 2018
- Women had better sexual desire, orgasm and satisfaction after Vitamin D supplementation – Feb 2018
- Global sperm count dropped by 59 percent in 40 years – meta-analysis Aug 2017
- Increased Testosterone and Erectile function, decreased weight with Vitamin D – March 2017
- Many fertility disorders associated with low vitamin D, still unsure how much is needed – Dec 2016
- Male fertility 4 X higher if high Vitamin D – Nov 2015
- Pregnancy success increased 30 percent if sunny (or vitamin D) one month earlier – June 2015
- Assisted Reproduction – 5 studies concluded vitamin D repletion helps – Review March 2015
- Erectile Dysfunction associated with low vitamin D in several studies
- Infertility rate is 2X higher for blacks than whites (no mention of vitamin D, nor the man) – April 2014
- Vitamin D protects against many types of health problems – review May 2013
- Male infertility associated with low vitamin D levels – Dec 2012
- IVF 4X more successful for white women with lots of vitamin D – Oct 2012
- Reproduction function in males improved by vitamin D – review Aug 2012
- Vitamin D - roles in women's reproductive health - Nov 2011