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Multiple Sclerosis prevention with high-dose vitamin D (in rats in this case) - July 2023

Vitamin D—An Effective Antioxidant in an Animal Model of Progressive Multiple Sclerosis - July 2023

Nutrients 2023, 15(15), 3309; https://doi.org/10.3390/nu15153309
by Michaela Tanja Haindl 1ORCID,Muammer Üçal 2ORCID,Willibald Wonisch 3ORCID,Michaela Lang 1,4,Marta Nowakowska 2ORCID,Milena Z. Adzemovic 5,Michael Khalil 1ORCID,Christian Enzinger 1 and Sonja Hochmeister 1,*
1 Department of Neurology, Medical University of Graz, 8036 Graz, Austria
2 Department of Neurosurgery, Medical University of Graz, 8036 Graz, Austria
3 Otto Loewi Research Center, Department of Physiological Medicine, Medical University of Graz, 8010 Graz, Austria
4 Faculty of Health, University of Applied Sciences Wiener Neustadt, Campus 1, 2700 Wiener Neustadt, Austria
5 Department of Clinical Neuroscience, Karolinska Institutet, 171 64 Stockholm, Sweden

Vitamin D (VD) is the most discussed antioxidant supplement for multiple sclerosis (MS) patients and many studies suggest correlations between a low VD serum level and onset and progression of the disease. While many studies in animals as well as clinical studies focused on the role of VD in the relapsing-remitting MS, knowledge is rather sparse for the progressive phase of the disease and the development of cortical pathology. In this study, we used our established rat model of cortical inflammatory demyelination, resembling features seen in late progressive MS, to address the question about whether VD could have positive effects on reducing cortical pathology, oxidative stress, and neurofilament light chain (NfL) serum levels. For this purpose, we used male Dark Agouti (DA) rats, with one group being supplemented with VD (400 IE per week; VD+) from the weaning on at age three weeks; the other group received standard rodent food. The rat brains were assessed using immunohistochemical markers against demyelination, microglial activation, apoptosis, neurons, neurofilament, and reactive astrocytes. To evaluate the effect of VD on oxidative stress and the antioxidant capacity, we used two different oxidized lipid markers (anti- Cu++ and HOCl oxidized LDL antibodies) along with colorimetric methods for protective polyphenols (PP) and total antioxidative capacity (TAC). NfL serum levels of VD+ and VD− animals were analyzed by fourth generation single-molecule array (SIMOA) analysis. We found significant differences between the VD+ and VD− animals both in histopathology as well as in all serum markers. Myelin loss and microglial activation is lower in VD+ animals and the number of apoptotic cells is significantly reduced with a higher neuronal survival. VD+ animals show significantly lower NfL serum levels, a higher TAC, and more PP. Additionally, there is a significant reduction of oxidized lipid markers in animals under VD supplementation. Our data thus show a positive effect of VD on cellular features of cortical pathology in our animal model, presumably due to protection against reactive oxygen species. In this study, VD enhanced remyelination and prevented neuroaxonal and oxidative damage, such as demyelination and neurodegeneration. However, more studies on VD dose relations are required to establish an optimal response while avoiding overdosing.
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Vitamin D in Multiple Sclerosis—Lessons From Animal Studies - Oct 2021

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VitaminDWiki – Multiple Sclerosis 32 percent less likely among those with more than 32 ng of vitamin D – Dec 2019

UV and Sunshine reduces MS risk

Other things also help

High Dose Vitamin D and cofactors

Number of MS studies which are also in other categories

  • 22 studies in Genetics - genes can restrict Vitamin D getting to the blood and to the cells
  • 12 studies in Vitamin D Receptor - gene which restricts D from getting to the cells
  • 7 studies in Vitamin D Binding Protein - gene which restricts D from getting to the cells
  • 21 studies in Ultraviolet light - may be even better than Vitamin D in preventing and treating MS
  • 9 studies in Omega-3 - which helps Vitamin D prevent and treat MS

VitaminDWiki – Overview MS and vitamin D contains

Clinical interventions have shown that Vitamin D can prevent, treat, and even cure Multiple Sclerosis, at a tiny fraction of the cost of the drugs now used to treat it, and without side effects.

Summary: lack of consensus on how much to prevent, treat, or cure MS.

Attached files

ID Name Comment Uploaded Size Downloads
19924 Lessons From Animal Studies.pdf admin 08 Aug, 2023 469.99 Kb 99
19923 MS rats_CompressPdf.pdf admin 08 Aug, 2023 834.80 Kb 88