Vitamin D receptor pathway is required for probiotic protection in colitis
American Journal of Physiology - Gastrointestinal and Liver Physiology Published 1 September 2015 Vol. 309 no. 5, G341-G349 DOI: 10.1152/ajpgi.00105.2015
Shaoping Wu, Sonia Yoon, Yong-Guo Zhang, Rong Lu, Yinglin Xia, Jiandi Wan, Elaine O. Petrof, Erika C. Claud, Di Chen, Jun Sun
Low expression of vitamin D receptor (VDR) and dysfunction of vitamin D/VDR signaling are reported in patients with inflammatory bowel disease (IBD); therefore, restoration of VDR function to control inflammation in IBD is desirable. Probiotics have been used in the treatment of IBD. However, the role of probiotics in the modulation of VDR signaling to effectively reduce inflammation is unknown.
We identified a novel role of probiotics in activating VDR activity, thus inhibiting inflammation, using cell models and VDR knockout mice.
We found that the probiotics Lactobacillus rhamnosus strain GG (LGG) and Lactobacillus plantarum (LP) increased VDR protein expression in both mouse and human intestinal epithelial cells. Using the VDR luciferase reporter vector, we detected increased transcriptional activity of VDR after probiotic treatment. Probiotics increased the expression of the VDR target genes, such as antimicrobial peptide cathelicidin, at the transcriptional level. Furthermore, the role of probiotics in regulating VDR signaling was tested in vivo using a Salmonella-colitis model in VDR knockout mice. Probiotic treatment conferred physiological and histologic protection from Salmonella-induced colitis in VDR+/+ mice, whereas probiotics had no effects in the VDR−/− mice. Probiotic treatment also enhanced numbers of Paneth cells, which secrete AMPs for host defense. These data indicate that the VDR pathway is required for probiotic protection in colitis. Understanding how probiotics enhance VDR signaling and inhibit inflammation will allow probiotics to be used effectively, resulting in innovative approaches to the prevention and treatment of chronic inflammation.
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See also VitaminDWiki
- Surprise:Three different types of vitamin D can activate the vitamin D receptor – Oct 2013
wonder how many other things can activate the VDR?
- 2,776 vitamin D receptor binding sites - April 2014
Pages listed in BOTH the categories Vitamin D Receptor and Antibiotics-Probiotics
Pages listed in BOTH the categories Vitamin D Receptor and Gut
- Crohn's and Vitamin D - many studies
- A healthy gut needs Vitamin D and a good vitamin D receptor – Sept 2022
- 14th activator of the Vitamin D Receptor – Butyrate (from gut bacteria, or supplement)
- Crohn’s Disease is associated with poor Vitamin D Receptor (many solutions) – April 2020
- Microbiomes of both gut and airway are affected by Vitamin D and Vitamin D Receptor – Nov 2018
- Vitamin D Receptor, the gut, the immune system, and the Middle East – May 2019
- Inflammatory bowel disease, gut bionome and Vitamin D Receptor – 2018
- Resveratrol Role in Autoimmune Disease-A Mini-Review. – Dec 2016
- Immunological effects of vitamin D and their relations to autoimmunity – March 2019
- Gut and airway bionome are affected by Vitamin D and Vitamin D Receptor – Nov 2018
- Inflammation and immune responses to Vitamin D (perhaps need to measure active vitamin D) – July 2017
- Ulcerative Colitis – half have poor level of Vitamin D Receptor vs only one in ten normally – June 2017
- Ulcerative colitis associated with both low Vitamin D and poor Vitamin D Receptors – Oct 2016
- Crohn's disease associated with 7.6X deactivation of Vitamin D receptor – July 2015