Photochem Photobiol. 2015 January ; 91(1): 201-209. doi:10.1in/php.12382.
Erin M. Burns, Craig A. Elmets, and Nabiha Yusuf nabiha at uab.edu.
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA
- Melanoma cancer growth slowed by increased Vitamin D Receptor (yet again) – Oct 2019
- Nonmelanoma Skin Cancer 2X more likely if poor Vitamin D Receptor – Oct 2017
- UVB improvements to Vitamin D receptor appear to improve melanoma survival – Oct 2017
- Skin Cancers, Vitamin D, Vitamin D Receptor and Genes – Jan 2015
- Malignant melanoma may be reduced by skin-activated vitamin D – Nov 2016
- Skin cancer 20 percent more likely with some Vitamin D receptor gene polymorphisms – Oct 2015
- Death from melanoma (without ulcers) greatly decreased if have lots of vitamin D receptors – May 2014
- Melanoma risk 2X to 4X higher if Vitamin D receptor genes had morphed – March 2014
- Vitamin D receptor may suppress skin cancer – Dec 2013
Vitamin D Receptor category has the following
Vitamin D tests cannot detect Vitamin D Receptor (VDR) problems
A poor VDR restricts Vitamin D from getting in the cells
It appears that 30% of the population has a poor VDR (40% of the Obese )
VDR at-home test $29 - results not easily understood in 2016
There are hints that you may have inherited a poor VDR
Compensate for poor VDR by increasing one or more:
|1) Vitamin D supplement|
Sun, Ultraviolet -B
| Vitamin D in the blood |
and thus in the cells
|2) Magnesium||Vitamin D in the blood |
AND in the cells
|3) Omega-3||Vitamin D in the cells|
|4) Resveratrol||Vitamin D Receptor|
|5) Intense exercise||Vitamin D Receptor|
|6) Get prescription for VDR activator|
|Vitamin D Receptor|
|7) Quercetin (flavonoid)||Vitamin D Receptor|
|8) Zinc is in the VDR||Vitamin D Receptor|
|9) Boron||Vitamin D Receptor ?, |
|10) Essential oils e.g. ginger, curcumin||Vitamin D Receptor|
|11) Progesterone||Vitamin D Receptor|
|12) Infrequent high concentration Vitamin D|
Increases the concentration gradient
|Vitamin D in the cells|
Note: If you are not feeling enough benefit from Vitamin D, you might try increasing VDR activation. You might feel the benefit within days of adding one or more of the above
Far healthier and stronger at age 72 due to supplements Includes 6 supplements which help the VDR
Vitamin D signaling plays a key role in various important processes, including cellular proliferation, differentiation, and apoptosis, immune regulation, hormone secretion, and skeletal health. Further, vitamin D production and supplementation have been shown to exert protective effects via an unknown signaling mechanism involving the vitamin D receptor (VDR) in several diseases and cancer types, including skin cancer. With over 3.5 million new diagnoses in 2 million patients annually, skin cancer is the most common cancer type in the United States. While ultraviolet B (UVB) radiation is the main etiologic factor for non-melanoma skin cancer (NMSC), UVB also induces cutaneous vitamin D production. This paradox has been the subject of contradictory findings in the literature in regards to amount of sun exposure necessary for appropriate vitamin D production, as well as any beneficial or detrimental effects of vitamin D supplementation for disease prevention. Further clinical and epidemiological studies are necessary to elucidate the role of vitamin D in skin carcinogenesis.
Over the last century, great progress has been made in the understanding of the formation and activation of vitamin D. However, it was not until after the cloning of the vitamin D receptor in 1987 and the discovery of the presence of the VDR in nearly all cells that both basic and clinical studies examined the non-skeletal-related effects of vitamin D, especially in chronic disease models. Systemic effects of vitamin D are shown in Table 2. Though there are many studies examining the relationship of vitamin D and cancer, confounding variables and small sample sizes make it difficult to determine significant and valid results. We attempt herein to focus on the relationship between vitamin D and skin cancer in an effort to present the existing literature and suggest future studies to elucidate and verify the details of the complex relationship.
Vitamin D, a prohormone and secosteroid, is produced in the skin, which is the only known organ system able to produce each major component involved in the vitamin D signaling pathway, in response to ultraviolet light B (UVB; 290-320nm) exposure (1-4). There are two main forms of vitamin D, which include animal-derived (cholecalciferol D3; from cholesterol) and plant-derived (ergocalciferol D2; from ergosterol). A summary of the different forms can be found in Table 1. Briefly, UV irradiation of 7-dehydrocholesterol produces vitamin D3, which is then metabolized through a hydroxylation process to 25- hydroxyvitamin D3 in the liver, and eventually to 1-alpha,25-dihydroxyvitamin D3 in the kidney, which is the metabolically active form that is produced on a systemic level (reviewed in (5)). While this metabolic process is usually carried out in the liver and kidney, animal studies have revealed the presence of hydroxylases in the epidermis, which may indicate that active conversion occurs in the skin as well, without passage through the liver (6, 7). Interestingly, circulating 25-D3 can be activated via 1-alpha hydroxylation in target tissues, which proceeds through the activity of CYP24, which is the classical pathway. Recently, an alternative in vivo pathway for activation of vitamin D3 (8) and D2 (9) was revealed, which begins with CYP11A1 activity and is further modified by CYP27B1 (10-12), and its activity in the skin is related to the expression of CYP11A1 in this organ (13, 14). Following activation, the metabolite, 1,25-D3 acts as the ligand for the vitamin D receptor.
The VDR, an intracellular receptor and transcription factor, belongs to a protein family that includes other receptors for steroid hormones, retinoids, isoprenoids, eicosanoids, and cholesterol metabolites (reviewed in (15)). The VDR is between 50-60kDa and contains a shorter N-terminal domain than many nuclear hormone receptors. The DNA-binding domain is the most conserved domain both among species and nuclear hormone receptors. The DNA-binding domain contains two zinc fingers, of which the N-terminal zinc finger is important for the specificity of DNA binding to the vitamin D response element (VDRE), and the other zinc finger is responsible for providing a heterodimerization site for the VDR and the retinoid X receptor (RXR). The ligand-binding domain is where 1,25-D3 binds and this region also is important for RXR heterodimerization. AF-2, the major activation domain, is found at the C-terminal end of the VDR and is necessary for steroid receptor coactivator (SRC) and VDR-interacting protein (DRIP) binding (16), which can therefore regulate the VDR. A murine study demonstrated that the VDR plays a key role in the action of vitamin D as evidenced by a severe vitamin D deficiency in animals with the VDR deleted (17). In the human body, the VDR is found in over 60 cell types (18), leading to the breadth of processes and diseases affected by vitamin D (19).
Activated vitamin D can function through two major pathways, genomic and nongenomic. The genomic pathway results in the activation of VDRE which influences the expression of over 900 genes important for various physiological processes that may ultimately result in carcinogenesis (20). The VDRE is activated by the VDR/RXR heterodimer and the binding of 1,25-D3 to the VDR. The non-genomic pathway consists of the intacrine actions of active vitamin D when it is synthesized from its precursor metabolite. Intracellular signaling cascades are stimulated when active vitamin D binds to the VDRs in the membrane, resulting in the regulation of chloride and calcium channel opening as well as further signaling molecule activation (21). This pathway may also be used for protection against DNA damage in addition to ionic homeostasis maintenance (22).
Studies have revealed that other receptors besides the classical vitamin D receptor are targets for activated forms of vitamin D. Originally discovered as a cell surface receptor for 1,25D3, the 1,25D3 membrane-associated rapid response steroid-binding (MARRS) receptor preferentially binds analogs of 1,25D3 that have a cis configuration, resembling hormones that have an intact B ring such as estradiol, rather than 1,25D3 itself, which has a broken B ring (23). In this calcium-dependent membrane-mediated pathway (24-26), 1,25D3 functions via a specific membrane-associated receptor protein disulfide isomerase family A, member 3 (Pdia3; also known as ERp57, ERp60, Grp58, and 1,25-MARRS) (27, 28) found in 50-100 nm lipid rafts enriched with both glycosphingolipids and cholesterol (29), which are called caveolae. The caveolae function as signaling platforms for several steroid hormones via their caveolin coat proteins.
Additionally, there are other nuclear receptors that are also targets for active forms of vitamin D, including the retinoic acid-related orphan receptors (ROR) alpha and gamma (30).
Regardless of ethnicity, circulating vitamin D levels are lower in non-Caucasians compared to Caucasians even though non-Caucasians exhibit increased bone mass (31). Interestingly, the lowest vitamin D levels in Caucasians are found in individuals with the fairest skin types, which may be due in part to lower amounts of UVB exposure in fair skinned individuals. Because decreased vitamin D levels have been linked with increased melanoma incidence as well as decreased survival (32), future studies are required to fully understand the implications of vitamin D supplementation and deficiency in terms of race and sun exposure requirements and recommendations (33).
In the United States and many countries in Europe, sufficient vitamin D levels are obtained via UVB-induced, cutaneous 7-dehydrocholesterol synthesis (90%) and nutrients found in the diet (10%) (34). There has been much discussion as to whether ingesting vitamin D from the diet or from a supplement is the same as producing vitamin D3 in the skin. It has been observed that when produced in the skin, circulating vitamin D lasts 2-3 times longer than when it is ingested orally (35). In the skin, previtamin D takes nearly 8 hours to be fully converted into metabolically active vitamin D (36, 37) and then it takes more time to enter the dermal capillary bed. 100% of Vitamin D produced in the skin can be bound to the vitamin D binding protein. In contrast, vitamin D obtained through the diet is incorporated into chylomicrons and transported into the lymphatic and subsequently into the venous system where only around 60% of the vitamin D is bound to the vitamin D binding protein while the remainder is rapidly cleared.
While obtaining vitamin D through UVB exposure is cheaper than purchasing supplements or a constant supply of vitamin D-rich foods, it is not without detrimental effects. It is well documented that both brief periods of high-intensity UVB exposure and UVB exposure have been linked with cutaneous inflammation and DNA damage that can contribute to skin carcinogenesis (38-42). In the 1970s, sunscreens were designed with the purpose of absorbing UVB radiation and therefore decreasing the harmful effects of the sun (43). Sunscreen that is labeled to have a sun protection factor (SPF) of 30 absorbs 95-98% of UVB radiation. The same sunscreen will also reduce the ability of the skin to synthesize metabolically active vitamin D by the same amount (18). One study demonstrated in a group of farmers with a NMSC history living in the Midwest that those who wore sunscreen exhibited significantly lower or deficient levels of vitamin D compared to the levels observed in the control group (44). In contrast, one study demonstrated that even with high levels of uv exposure, subjects were still vitamin D-deficient. Supplementing diet with vitamin D-rich foods is a better way to eliminate vitamin D deficiency, especially in darker skinned individuals, where cutaneous vitamin D production is not as effective with UVB exposure. There is a known and epidemiologically supported link between increased UVB exposure and NMSC development, so continuing safe sun practices will decrease NMSC risk and will not affect vitamin D status (45). It has been suggested that the effective slip, slap, slop educational campaign in Australia has resulted in vitamin D deficiency of over 40% of the population (46-49), and the subsequent recommendation of the Australian Dermatology Society for sensible sun exposure as a vitamin D source. A three-part strategy has been suggested for implementation to reduce vitamin D deficiency worldwide that consists of sensible sun exposure, the consumption of vitamin D-rich or fortified foods as well as a vitamin D supplement. Taken together, continuing safe sun practices along with the intake of vitamin D-rich foods and a vitamin D supplement, may help to decrease NMSC risk.
The effects of vitamin D on immune responses remain unclear. While some studies demonstrate that topical vitamin D can actually decrease against UV-induced responses such as DNA damage and immunosuppression, other studies have shown that vitamin D plays a key role in the development of T regulatory cells, which utilizes signaling through the vitamin D receptor (50), as well as other important immune cells (51). This mechanism is dependent on Langerhans cells (50), which ultimately results in the same outcome as UVB exposure used as treatment in psoriasis, which is an immunodermatological disease. It has been suggested that topical use of vitamin D may be a substitute for UVB treatment, which would eliminate DNA damage caused by UVB exposure, and in fact, vitamin D and its analogs may promote DNA damage repair responses (52). Additionally, signaling through the VDR, of which several polymorphisms have been reported and investigated, may provide a possible explanation for the genetic susceptibility to immunodermatological diseases such as non-melanoma and melanoma skin cancers in addition to psoriasis. Vitamin D deficiency may therefore decrease immune cell development and lead to immunosuppression, which has been shown to lead to increased NMSC development, especially in immunosuppressed patients such as solid organ transplant patients, who have 60-250-fold increased risk of developing skin cancer (53-58). Interestingly, regulatory T cells, the development of which is modulated in part by vitamin D signaling, are important mediators of UVB-induced immunosuppression (59-65), which can lead to skin cancer development.
Vitamin D production and supplementation have been shown to exert protective effects via an unknown signaling mechanism involving the VDR in several diseases and cancer types, including skin cancer. With over 3.5 million new diagnoses in 2 million patients annually, skin cancer is the most common cancer type in the United States (66). While ultraviolet B (UVB) radiation is the main etiologic factor for non-melanoma skin cancer (NMSC), UVB also induces cutaneous vitamin D production. Epidemiological studies have reported a relationship between vitamin D synthesis and UV exposures (2, 4, 67). Breast cancer incidence has been reported to be 50% less in women with high levels of UVB exposure as compared to women with lower exposure (67). Likewise, men with high UVB exposure had a 50% lower incidence of fatal prostate cancer. Deficiency of vitamin D has been strongly associated with both the development and progression of several types of cancers (68-70). Interestingly, one study in postmenopausal women demonstrated that vitamin D3 and calcium supplementation resulted in a substantial reduction of all cancer risks (71). While no studies have significantly linked vitamin D supplementation to NMSC prevention or treatment in large human populations, there is a link between vitamin D and several other cancer types (67, 72-74).
In a nested case-control study of elderly men, a lower occurrence of NMSC was found in subjects with the highest serum concentration of the previtamin D metabolite compared to subjects with the lowest concentrations (75). Further, increased previtamin D levels in plasma have been associated with better survival as well as decreased metastasis (22, 70, 76, 77). Additionally, vitamin D3 and 1,25-D3 levels have been shown to help determine survival and metastasis prognoses for skin cancer as well as cancers of the lung, breast, and prostate (74, 75, 77-83).
Vitamin D has also been shown to regulate the cell cycle by altering expression of cell cycle control genes (84, 85). Both human and animal studies have demonstrated a key role of the vitamin D pathway for skin homeostasis and keratinocyte growth and differentiation (86), as well as skin carcinogenesis (87-90).
In vitro studies have suggested that vitamin D supplementation is facilitated via the non- genomic pathway as illustrated by the use of analogs as agonists and antagonists for DNA damage prevention (91). Further studies in the hairless mouse model support these findings and suggest a mechanism for preventing UV-induced skin carcinogenesis with topical 1,25- D3 treatment (90). It has also been suggested that vitamin D produced endogenously following exposure to UVB may result in a protective, photoadaptive effect against exposure to solar radiation following the initial exposure.
Several studies have implicated a role for the vitamin D3/1,25-D3/VDR axis in the determination of malignant melanoma as well as various other systemic cancers (summarized in (92)). VDR expression can be detected in melanoma cells and both the prodifferentiation and antiproliferative effects of 1,25-D3 have been shown in human melanoma cells (93-95). In a xenograft mouse model, 1,25-D3 has been shown to suppress melanoma growth using VDR-positive human-derived malignant melanomas (96). In a human malignant melanoma cell line, 1,25-D3 induced apoptosis; however, another cell line was unresponsive despite expressing the VDR (97). CYP24A1 mRNA has been shown to be upregulated with vitamin D supplementation in melanoma cells, which deactivates MAPK signaling and may partially control the anti-proliferative effects of vitamin D (98). Higher 25-D3 levels were associated with thinner tumors (lower Breslow thickness) at diagnosis (32). Vitamin D levels were also independently protective of relapse and death. Low levels of circulating vitamin D in patients with stage IV melanoma compared to stage I also developed earlier distant metastatic disease (99). Further, decreased or lost expression of VDR or CYP27B1 has been shown to lead to the progression of melanocytic tumors (100) and melanoma (101), and to decrease overall and disease-free survival of melanoma patients (102).
In a randomized, placebo-controlled trial of postmenopausal women aged 50-79 enrolled in the Women's Health Initiative, the authors evaluated effects of vitamin D3 combined with calcium supplementation on skin cancer for a mean follow-up of 7 years. Supplementation (1000mg Ca + 400 IU vit D3 daily) did not reduce overall NMSC or melanoma incidence; however, in women with NMSC personal history, supplementation reduced melanoma risk (103). The amount of vitamin D3 used in this study has been argued to be quite low, and therefore results may be seen with high supplementary levels.
An individual's susceptibility to skin cancer can be ascertained by the ability of vitamin D binding to their vitamin D receptor (VDR). Most of the studies have been conducted in European Caucasians, where small nucleotide polymorphisms (SNPs) were assessed for determining the risk of non-melanoma (SCCs and BCCs) and melanoma skin cancer in individuals. The SNPs in VDR have been associated with both the occurrence and outcome of skin cancer. More than sixty VDR polymorphisms have been discovered and are present in and around exons 2-9 and in the 3’UTR region of the promoter. Due to difficulty in analysis of these VDR SNPs, only few polymorphisms of this large gene have been studied extensively. Only few epidemiological studies have directly addressed the relationship between VDR SNPS and skin cancer. The most well studied VDR SNPs in skin cancers are Taq1, Bsm1, Apa1, and Fok1 (104).
The TaqI polymorphism (rs731236) is a restriction fragment length polymorphism (RFLP), which occurs at codon 352 in exon 9 of the VDR gene. The products are digested into 2 fragments of 495 and 245 bp (T allele: absence of the restriction site) or 3 fragments of 290, 245 and 205 bp (t allele: presence of the restriction site) depending on the presence or absence of a Taq1 restriction site in each allele. Individuals are usually classified as TT, Tt or tt. The TT allele has been shown to be associated with lower circulating levels of active vitamin D3 (105-107). The TaqI polymorphism leads to a silent codon change (from ATT to ATC, which both result in an isoleucine at codon 352) (105).
The BsmI polymorphism (rs1544410) is an RFLP in intron 8 at the 3' end of the VDR gene. It is considered to be a silent SNP because it does not change the amino acid sequence of the encoded protein (108). However, it can affect mRNA stability and change the expression of gene (109).
The ApaI polymorphism (rs7975232) is located in intron 8 at the 3’ end of the VDR gene, like the BsmI polymorphism (110).
The FokI polymorphism (rs2228570, formally known as rs10735810) is located at the first potential start site (111, 112), which can be detected using the Fok1 restriction enzyme by RFLP (113). It alters an ACG codon that is located ten base pairs upstream from the translation start codon, thus resulting in the generation of an additional start codon. If the translation is initiated from this alternative site (thymine variant), it results in the generation of a longer VDR protein. This polymorphism is referred to as the f allele (112). However, the f allele exerts less transcriptional activity (114), with the F variant being 1.7-fold more active (113, 115, 116). Fok1 polymorphism is the only known VDR gene polymorphism that actually results in the generation of an altered protein.
VDR SNPs in non-melanoma skin cancers
There has been a lot of variation in the studies linking VDR SNPs with SCCs and BCCs. When the association of VDR gene with solar keratosis (SK), a biomarker for skin cancer, was examined with skin phenotype, a significant difference in genotype frequencies of the TaqI polymorphism was found between affected and unaffected populations. The TT/tt genotype group was associated with a seven fold increase, whereas Tt group had a four fold increase in odds of being affected by SK. Fair-skinned people with VDR-Apa AA/aa genotypes had about an eightfold increase in odds of being affected by SK compared with a fivefold increase in individuals with the Aa genotype and fair skin. This study shows that the homozygote genotypes increase the odds of SK, thus suggesting that intermediate VDR activity is important in protection or that the heterodimer formed by a heterozygous genotype may have an altered binding potential (117). A meta-analysis of VDR SNPs showed that Fok1, Taq1 and Apa1 may be the susceptibility biomarkers for skin cancer in Caucasians. No significant association was observed between the Bsm1 polymorphism and skin cancer risk (118). Another study evaluating the hedgehog pathway that drives most BCCs, and pathway-related gene variants contribute to the increased risk of subsequent cancers among those with a history of BCC. No significant associations were observed for any of the hedgehog pathway SNPs, or for the FAS SNPs. Thus, hedgehog pathway gene SNPs studied, along with the VDR and FAS SNPs studied, are not strongly associated with the BCC cancer-prone phenotype (119). In a German study, associations indicated that the Apa1 and Taq1 genotypes may be important for development of BCCs, but not for SCCs. Comparison of the VDR genotype frequencies by age (younger than 60 years vs. 60 years or older) revealed that it had no association with SNPs in patients with BCCs or SCCs (104).
UV radiation has a major influence on folate and vitamin D as UV radiation can break down plasma folate, and synthesize vitamin D in skin. Folate metabolism is involved in DNA synthesis and repair, whereas vitamin D can inhibit proliferation. Both these nutrients have important implications in skin carcinogenesis. In a study in Polish population, it was found that the presence of the TT genotype in the FokI VDR polymorphism resulted in a >10-fold higher risk of BCC development. The CT genotype in 677C/T MTHFR polymorphism and CC genotype in 1286A/C MTHFR (methylenetetrahydrofolate reductase) polymorphism also significantly increased the risk of BCC development. The expression of the VDR and MTHFR proteins was significantly higher in BCCs of the patients than in the healthy skin of the controls (120). In a nested case control Nurses’ Health Study on melanoma and nonmelanoma skin cancers, polymorphisms in MTHFR and VDR were associated with dietary intakes of folate and vitamin D in skin cancer development, especially for SCC (121).
VDR SNPs in malignant melanoma
Melanoma is one of the most aggressive human cancers. The vitamin D system contributes to the pathogenesis and prognosis of malignancies including cutaneous melanoma. An expression of the vitamin D receptor (VDR) and an anti-proliferative effect of vitamin D in melanocytes and melanoma cells have been shown in vitro. Studies examining associations of polymorphisms in genes coding for vitamin D metabolism-related proteins (la- hydroxylase [CYP27B1], 1,25(OH)(2)D-24hydroxylase [CYP24A1], vitamin D-binding protein [VDBP]) found no association of polymorphisms tested with melanoma risk as well as prognosis in logistic and linear regression models (122). In a large, international, multinational population-based case control study examining 38 VDR gene SNPs with known or suspected impact on VDR activity, haplotype tagging SNPs with > 10% minor allele frequency in Caucasians, and SNPs reported as significant in other association studies were examined. Eight of 38 SNPs in the promoter, coding, and 3’ gene regions were individually significantly associated with multiple primary melanoma after adjusting for covariates. The estimated increase in risk for individuals who were homozygous for the minor allele ranged from 25 to 33% for six polymorphisms: rs10875712, rs4760674, rs7139166, rs4516035, rs11168287 and rs1544410; for two polymorphisms, homozygous carriers had a decreased risk: rs7305032 and rs7965281. There were false positive findings because of multiple comparisons; however, the eight significant SNPs in this study outnumbered the two significant tests expected to occur by chance, thus indicating a possible role of VDR in melanomagenesis (123). In a study in Spanish population, an association was found between polymorphisms in GC and melanoma risk, thus confirming studies from different populations (106). Only the BsmI variant was found to be associated with multiple primary melanoma. Their results suggested that risk of multiple primary melanoma was increased in people who have the BsmI variant of VDR (124). The association between Bsm1 variant and melanoma led to the theory that sun exposure may have a protective effect against melanoma through activation of the vitamin D system (125). This study examined the association between BsmI polymorphism and prevalence of malignant melanoma together with Breslow thickness. In addition, they also determined FokI SNP in this population. All cases and controls were found to be in Hardy-Weinberg equilibrium for BsmI, FokI and A-1012G. Significant associations were found between the BsmI bb genotype frequency and malignant melanoma (P = 0.02) along with Breslow thickness (P = 0.001). This same pattern was not observed for the FokI or A-1012G polymorphisms. Multivariate logistic regression analysis confirmed these significant results after they were corrected for age, gender, skin type and localization of malignant melanoma. Additional studies need to be done to verify this variant as a possible risk marker for malignant melanoma and its aggressiveness. However, it is possible that the real association may be due to other unknown genes linked to the BsmI b allele (126).
Non-calcemic vitamin D analogs have been investigated and have been shown to have antiproliferative, anti-inflammatory, and anti-fibrosing effects (127-129), resulting in a potential area for therapeutic interventions. Utilizing inhibitors of melanogenesis may be used in an attempt to improve the efficacy of various melanoma therapies (130). It has also been suggested that melatonin and vitamin D should be used as adjuvants due to their protective and anti-cancer properties as a therapy for advanced melanoma. This approach could take advantage of the easily monitored toxicity of vitamin D and the non-toxic melatonin, in attempt to prevent recurrent or progressing disease.
While vitamin D and the resultant signaling cascade play key roles in normal cellular processes and prevent against bone-related disorders, the topic of cutaneous health has remained controversial in terms of detrimental effects of the UVB exposure necessary for the production of metabolically active vitamin D in the skin. While several studies have demonstrated the benefits of adequate circulating vitamin D levels, there is a clear link between sun exposure and skin carcinogenesis. Vitamin D supplementation has also been linked to decreased cancer incidence as well as improved outcome and survival, including in skin cancer, however there are also studies that do not support this correlation. Difficulties in translating findings to large-scale, human studies include small study sizes, and the number of confounding factors present in each study. Future studies utilizing basic research, clinical interventions, and epidemiological studies are necessary to elucidate the intricacies of the relationship between vitamin D levels and skin cancer development in terms of sunscreen use, diet, and the use of vitamin D supplements.
Nabiha Yusuf received her MS degree in Microbiology, and PhD degree in the area of Immunology from India. Dr. Yusuf joined the department of Dermatology at the University of Alabama at Birmingham in 2001 as a Postdoctoral Fellow.
After completing her postdoctoral training in the laboratory of Dr. Craig A. Elmets, she became an Instructor in 2005. She received a Research Career Development Award from Dermatology Foundation, and became an Assistant Professor in 2009. Her major research interests focus on the role of innate immune system on the environmental influences such and chemical carcinogens and ultraviolet (UV) radiation on the development of skin cancer. Her other research interests include the modulation of non-melanoma skin cancer by dietary supplements, and the microbiome. She is currently collaborating with Dr. Elmets to delineate the role of vitamin D receptor polymorphisms in nonmelanoma skin cancer.
Erin Burns received her PhD from The Ohio State University in 2013, where her dissertation research investigated sex differences in non-melanoma skin cancer, specifically focusing on anti-inflammatory and antioxidant compounds for UVB-induced squamous cell carcinoma prevention. She is currently a postdoctoral fellow in the Cancer Prevention and Control Training Program supported by NCI/NIH, under the mentorship of Dr. Nabiha Yusuf and Dr. Craig Elmets. Her current research is focused on non-melanoma skin cancer prevention and control, investigating the effects of dietary supplements, genetic markers, and the microbiome as new targets for risk assessment or therapeutic interventions.
- Bikle DD, Chang S, Crumrine D, Elalieh H, Man MQ, Dardenne O, Xie Z, Arnaud RS, Feingold K, Elias PM. Mice lacking 25OHD 1alpha-hydroxylase demonstrate decreased epidermal differentiation and barrier function. The Journal of steroid biochemistry and molecular biology. 2004; 89-90:347-353. [PubMed: 15225799]
- Holick MF. High prevalence of vitamin D inadequacy and implications for health. Mayo Clinic proceedings. 2006; 81:353-373. [PubMed: 16529140]
- Grant WB, Cross HS, Garland CF, Gorham ED, Moan J, Peterlik M, Porojnicu AC, Reichrath J, Zittermann A. Estimated benefit of increased vitamin D status in reducing the economic burden of disease in western Europe. Progress in biophysics and molecular biology. 2009; 99:104-113. [PubMed: 19268496]
- Webb AR. Who, what, where and when-influences on cutaneous vitamin D synthesis. Progress in biophysics and molecular biology. 2006; 92:17-25. [PubMed: 16766240]
- DeLuca HF. Overview of general physiologic features and functions of vitamin D. The American journal of clinical nutrition. 2004; 80:1689S-1696S. [PubMed: 15585789]
- Bikle DD, Nemanic MK, Gee E, Elias P. 1,25-Dihydroxyvitamin D3 production by human keratinocytes. Kinetics and regulation. The Journal of clinical investigation. 1986; 78:557-566. [PubMed: 2426308]
- Bikle DD, Nemanic MK, Whitney JO, Elias PW. Neonatal human foreskin keratinocytes produce 1,25-dihydroxyvitamin D3. Biochemistry. 1986; 25:1545-1548. [PubMed: 2423114]
- Slominski A, Semak I, Zjawiony J, Wortsman J, Li W, Szczesniewski A, Tuckey RC. The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism. The FEBS journal. 2005; 272:4080-4090. [PubMed: 16098191]
- Slominski A, Semak I, Wortsman J, Zjawiony J, Li W, Zbytek B, Tuckey RC. An alternative pathway of vitamin D metabolism. Cytochrome P450scc (CYP11A1)-mediated conversion to 20- hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2. The FEBS journal. 2006; 273:2891-2901. [PubMed: 16817851]
- Slominski AT, Kim TK, Janjetovic Z, Tuckey RC, Bieniek R, Yue J, Li W, Chen J, Nguyen MN, Tang EK, Miller D, Chen TC, Holick M. 20-Hydroxyvitamin D2 is a noncalcemic analog of vitamin D with potent antiproliferative and prodifferentiation activities in normal and malignant cells. American journal of physiology. Cell physiology. 2011; 300:C526-541. [PubMed: 21160030]
- Slominski AT, Kim TK, Li W, Yi AK, Postlethwaite A, Tuckey RC. The role of CYP11A1 in the production of vitamin D metabolites and their role in the regulation of epidermal functions. The Journal of steroid biochemistry and molecular biology. 2014; 144(Pt A):28-39. [PubMed: 24176765]
- Slominski AT, Kim TK, Shehabi HZ, Semak I, Tang EK, Nguyen MN, Benson HA, Korik E, Janjetovic Z, Chen J, Yates CR, Postlethwaite A, Li W, Tuckey RC. In vivo evidence for a novel pathway of vitamin D(3) metabolism initiated by P450scc and modified by CYP27B1. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2012; 26:3901-3915. [PubMed: 22683847]
- Slominski A, Ermak G, Mihm M. ACTH receptor, CYP11A1, CYP17 and CYP21A2 genes are expressed in skin. The Journal of clinical endocrinology and metabolism. 1996; 81:2746-2749. [PubMed: 8675607]
- Slominski A, Zjawiony J, Wortsman J, Semak I, Stewart J, Pisarchik A, Sweatman T, Marcos J, Dunbar C, C. T. R. A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin. European journal of biochemistry / FEBS. 2004; 271:4178-4188. [PubMed: 15511223]
- Jones G, Strugnell SA, DeLuca HF. Current understanding of the molecular actions of vitamin D. Physiological reviews. 1998; 78:1193-1231. [PubMed: 9790574]
- Rachez C, Gamble M, Chang CP, Atkins GB, Lazar MA, Freedman LP. The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes. Molecular and cellular biology. 2000; 20:2718-2726. [PubMed: 10733574]
- Li YC, Pirro AE, Amling M, Delling G, Baron R, Bronson R, Demay MB. Targeted ablation of the vitamin D receptor: an animal model of vitamin D-dependent rickets type II with alopecia. Proceedings of the National Academy of Sciences of the United States of America. 1997; 94:9831-9835. [PubMed: 9275211]
- Holick MF, Chen TC, Lu Z, Sauter E. Vitamin D and skin physiology: a D-lightful story. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2007; 22(Suppl 2):V28-33.
- Walters MR. Newly identified actions of the vitamin D endocrine system. Endocrine reviews. 1992; 13:719-764. [PubMed: 1333949]
- Schuster I. Cytochromes P450 are essential players in the vitamin D signaling system. Biochimica et biophysica acta. 2011; 1814:186-199. [PubMed: 20619365]
- Norman AW, Mizwicki MT, Norman DP. Steroid-hormone rapid actions, membrane receptors and a conformational ensemble model. Nature reviews. Drug discovery. 2004; 3:27-41. [PubMed: 14708019]
- Deeb KK, Trump DL, Johnson CS. Vitamin D signalling pathways in cancer: potential for anticancer therapeutics. Nature reviews. Cancer. 2007; 7:684-700. [PubMed: 17721433]
- Nemere I, Dormanen MC, Hammond MW, Okamura WH, Norman AW. Identification of a specific binding protein for 1 alpha,25-dihydroxyvitamin D3 in basal-lateral membranes of chick intestinal epithelium and relationship to transcaltachia. The Journal of biological chemistry. 1994; 269:23750-23756. [PubMed: 8089147]
- Nemere I, Norman AW. The rapid, hormonally stimulated transport of calcium (transcaltachia). Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 1987; 2:167-169.
- Boyan BD, Schwartz Z, Swain LD, Bonewald LF, Khare A. Regulation of matrix vesicle metabolism by vitamin D metabolites. Connective tissue research. 1989; 22:3-16. discussion 53-61. [PubMed: 2689084]
- Khanal RC, Nemere I. The ERp57/GRp58/1,25D3-MARRS receptor: multiple functional roles in diverse cell systems. Current medicinal chemistry. 2007; 14:1087-1093. [PubMed: 17456022]
- Chen J, Olivares-Navarrete R, Wang Y, Herman TR, Boyan BD, Schwartz Z. Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts. The Journal of biological chemistry. 2010; 285:37041-37050. [PubMed: 20843786]
- Nemere I, Farach-Carson MC, Rohe B, Sterling TM, Norman AW, Boyan BD, Safford SE. Ribozyme knockdown functionally links a 1,25(OH)2D3 membrane binding protein (1,25D3- MARRS) and phosphate uptake in intestinal cells. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101:7392-7397. [PubMed: 15123837]
- Thomas CM, Smart EJ. Caveolae structure and function. Journal of cellular and molecular medicine. 2008; 12:796-809. [PubMed: 18315571]
- Slominski AT, Kim TK, Takeda Y, Janjetovic Z, Brozyna AA, Skobowiat C, Wang J,
- Postlethwaite A, Li W, Tuckey RC, Jetten AM. RORalpha and ROR gamma are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014; 28:2775-2789. [PubMed: 24668754]
- Nam HS, Kweon SS, Choi JS, Zmuda JM, Leung PC, Lui LY, Hill DD, Patrick AL, Cauley JA. Racial/ethnic differences in bone mineral density among older women. Journal of bone and mineral metabolism. 2013; 31:190-198. [PubMed: 23143509]
- Newton-Bishop JA, Beswick S, Randerson-Moor J, Chang YM, Affleck P, Elliott F, Chan M, Leake S, Karpavicius B, Haynes S, Kukalizch K, Whitaker L, Jackson S, Gerry E, Nolan C, Bertram C, Marsden J, Elder DE, Barrett JH, Bishop DT. Serum 25-hydroxyvitamin D3 levels are associated with breslow thickness at presentation and survival from melanoma. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 2009; 27:5439-5444. [PubMed: 19770375]
- Bataille V. Sun exposure, sunbeds and sunscreens and melanoma. What are the controversies? Current oncology reports. 2013; 15:526-532. [PubMed: 24142142]
- Norman AW. Sunlight, season, skin pigmentation, vitamin D, and 25-hydroxyvitamin D: integral components of the vitamin D endocrine system. The American journal of clinical nutrition. 1998; 67:1108-1110. [PubMed: 9625080]
- Haddad JG, Matsuoka LY, Hollis BW, Hu YZ, Wortsman J. Human plasma transport of vitamin D after its endogenous synthesis. The Journal of clinical investigation. 1993; 91:2552-2555. [PubMed: 8390483]
- Tian XQ, Chen TC, Lu Z, Shao Q, Holick MF. Characterization of the translocation process of vitamin D3 from the skin into the circulation. Endocrinology. 1994; 135:655-661. [PubMed: 8033813]
- Tian XQ, Holick MF. A liposomal model that mimics the cutaneous production of vitamin D3. Studies of the mechanism of the membrane-enhanced thermal isomerization of previtamin D3 to vitamin D3. The Journal of biological chemistry. 1999; 274:4174-4179. [PubMed: 9933613]
- Marks R. Role of childhood in the development of skin cancer. Australian paediatric journal. 1988; 24:337-338. [PubMed: 3242480]
- Marks R, Jolley D, Lectsas S, Foley P. The role of childhood exposure to sunlight in the development of solar keratoses and non-melanocytic skin cancer. The Medical journal of Australia. 1990; 152:62-66. [PubMed: 2296232]
- Gallagher RP, Hill GB, Bajdik CD, Coldman AJ, Fincham S, McLean DI, Threlfall WJ. Sunlight exposure, pigmentation factors, and risk of nonmelanocytic skin cancer. II. Squamous cell carcinoma. Archives of dermatology. 1995; 131:164-169. [PubMed: 7857112]
- Gallagher RP, Hill GB, Bajdik CD, Fincham S, Coldman AJ, McLean DI, Threlfall WJ. Sunlight exposure, pigmentary factors, and risk of nonmelanocytic skin cancer. I. Basal cell carcinoma. Archives of dermatology. 1995; 131:157-163. [PubMed: 7857111]
- Strickland PT, Vitasa BC, West SK, Rosenthal FS, Emmett EA, Taylor HR. Quantitative carcinogenesis in man: solar ultraviolet B dose dependence of skin cancer in Maryland watermen. Journal of the National Cancer Institute. 1989; 81:1910-1913. [PubMed: 2593168]
- Matsuoka LY, Ide L, Wortsman J, MacLaughlin JA, Holick MF. Sunscreens suppress cutaneous vitamin D3 synthesis. The Journal of clinical endocrinology and metabolism. 1987; 64:1165-1168. [PubMed: 3033008]
- Matsuoka LY, Wortsman J, Hollis BW. Lack of effect of exogenous calcitriol on the cutaneous production of vitamin D3. The Journal of clinical endocrinology and metabolism. 1988; 66:451453. [PubMed: 2828412]
- Reddy KK, Gilchrest BA. Iatrogenic effects of photoprotection recommendations on skin cancer development, vitamin D levels, and general health. Clinics in dermatology. 2011; 29:644-651. [PubMed: 22014986]
- Looker AC, Johnson CL, Lacher DA, Pfeiffer CM, Schleicher RL, Sempos CT. Vitamin D status: United States, 2001-2006. NCHS data brief. 2011:1-8. [PubMed: 21592422]
- Hossein-nezhad A, Holick MF. Optimize dietary intake of vitamin D: an epigenetic perspective. Current opinion in clinical nutrition and metabolic care. 2012; 15:567-579. [PubMed: 23075936]
- Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, Murad MH, Weaver CM. Guidelines for preventing and treating vitamin D deficiency and insufficiency revisited. The Journal of clinical endocrinology and metabolism. 2012; 97:1153-1158. [PubMed: 22442274]
- Daly RM, Gagnon C, Lu ZX, Magliano DJ, Dunstan DW, Sikaris KA, Zimmet PZ, Ebeling PR, Shaw JE. Prevalence of vitamin D deficiency and its determinants in Australian adults aged 25 years and older: a national, population-based study. Clinical endocrinology. 2012; 77:26-35. [PubMed: 22168576]
- Schwarz A, Navid F, Sparwasser T, Clausen BE, Schwarz T. 1,25-dihydroxyvitamin D exerts similar immunosuppressive effects as UVR but is dispensable for local UVR-induced immunosuppression. The Journal of investigative dermatology. 2012; 132:2762-2769. [PubMed: 22854622]
- Palmer MT, Lee YK, Maynard CL, Oliver JR, Bikle DD, Jetten AM, Weaver CT. Lineage-specific effects of 1,25-dihydroxyvitamin D(3) on the development of effector CD4 T cells. The Journal of biological chemistry. 2011; 286:997-1004. [PubMed: 21047796]
- Xu H, Elmets CA. UVB immunosuppression: vitamin D or not vitamin D? That is the question. The Journal of investigative dermatology. 2012; 132:2676-2678. [PubMed: 23187109]
- Bouwes Bavinck JN, Robertson I, Wainwright RW, Green A. Excessive numbers of skin cancers and pre-malignant skin lesions in an Australian heart transplant recipient. British heart journal. 1995; 74:468-470. [PubMed: 7488467]
- Caforio AL, Fortina AB, Piaserico S, Alaibac M, Tona F, Feltrin G, Pompei E, Testolin L, Gambino A, Volta SD, Thiene G, Casarotto D, Peserico A. Skin cancer in heart transplant recipients: risk factor analysis and relevance of immunosuppressive therapy. Circulation. 2000; 102:III222-227. [PubMed: 11082391]
- Espana A, Redondo P, Fernandez AL, Zabala M, Herreros J, Llorens R, Quintanilla E. Skin cancer in heart transplant recipients. Journal of the American Academy of Dermatology. 1995; 32:458465. [PubMed: 7868716]
- Ong CS, Keogh AM, Kossard S, Macdonald PS, Spratt PM. Skin cancer in Australian heart transplant recipients. Journal of the American Academy of Dermatology. 1999; 40:27-34. [PubMed: 9922009]
- Divakar D, Bailey RR, Lynn KL, Robson RA. Long term complications following renal transplantation. The New Zealand medical journal. 1991; 104:352-354. [PubMed: 1891132]
- Couetil JP, McGoldrick JP, Wallwork J, English TA. Malignant tumors after heart transplantation. The Journal of heart transplantation. 1990; 9:622-626. [PubMed: 2277298]
- Kripke ML. Antigenicity of murine skin tumors induced by ultraviolet light. Journal of the National Cancer Institute. 1974; 53:1333-1336. [PubMed: 4139281]
- Schwarz A, Maeda A, Wild MK, Kernebeck K, Gross N, Aragane Y, Beissert S, Vestweber D, Schwarz T. Ultraviolet radiation-induced regulatory T cells not only inhibit the induction but can suppress the effector phase of contact hypersensitivity. Journal of immunology. 2004; 172:10361043.
- Elmets CA, Bergstresser PR, Tigelaar RE, Wood PJ, Streilein JW. Analysis of the mechanism of unresponsiveness produced by haptens painted on skin exposed to low dose ultraviolet radiation. The Journal of experimental medicine. 1983; 158:781-794. [PubMed: 6193234]
- Rivas JM, Ullrich SE. Systemic suppression of delayed-type hypersensitivity by supernatants from UV-irradiated keratinocytes. An essential role for keratinocyte-derived IL-10. Journal of immunology. 1992; 149:3865-3871.
- Fisher MS, Kripke ML. Further studies on the tumor-specific suppressor cells induced by ultraviolet radiation. Journal of immunology. 1978; 121:1139-1144.
- Schwarz T. 25 years of UV-induced immunosuppression mediated by T cells-from disregarded T suppressor cells to highly respected regulatory T cells. Photochemistry and photobiology. 2008; 84:10-18. [PubMed: 18173696]
- Moodycliffe AM, Nghiem D, Clydesdale G, Ullrich SE. Immune suppression and skin cancer development: regulation by NKT cells. Nature immunology. 2000; 1:521-525. [PubMed: 11101875]
- Rogers HW, Weinstock MA, Harris AR, Hinckley MR, Feldman SR, Fleischer AB, Coldiron BM. Incidence estimate of nonmelanoma skin cancer in the United States, 2006. Archives of dermatology. 2010; 146:283-287. [PubMed: 20231499]
- Grant WB. Relation between prediagnostic serum 25-hydroxyvitamin D level and incidence of breast, colorectal, and other cancers. Journal of photochemistry and photobiology. B, Biology. 2010; 101:130-136.
- Baeke F, van Etten E, Gysemans C, Overbergh L, Mathieu C. Vitamin D signaling in immune- mediated disorders: Evolving insights and therapeutic opportunities. Molecular aspects of medicine. 2008; 29:376-387. [PubMed: 18621414]
- Balasubramanian S, Eckert RL. Keratinocyte proliferation, differentiation, and apoptosis-differential mechanisms of regulation by curcumin, EGCG and apigenin. Toxicology and applied pharmacology. 2007; 224:214-219. [PubMed: 17493651]
- Banerjee P, Chatterjee M. Antiproliferative role of vitamin D and its analogs--a brief overview. Molecular and cellular biochemistry. 2003; 253:247-254. [PubMed: 14619976]
- Lappe JM, Travers-Gustafson D, Davies KM, Recker RR, Heaney RP. Vitamin D and calcium supplementation reduces cancer risk: results of a randomized trial. The American journal of clinical nutrition. 2007; 85:1586-1591. [PubMed: 17556697]
- Holick MF. Vitamin D: its role in cancer prevention and treatment. Progress in biophysics and molecular biology. 2006; 92:49-59. [PubMed: 16566961]
- Gorham ED, Garland CF, Garland FC, Grant WB, Mohr SB, Lipkin M, Newmark HL,
- Giovannucci E, Wei M, Holick MF. Optimal vitamin D status for colorectal cancer prevention: a quantitative meta analysis. American journal of preventive medicine. 2007; 32:210-216.
- [PubMed: 17296473]
- Porojnicu A, Robsahm TE, Berg JP, Moan J. Season of diagnosis is a predictor of cancer survival. Sun-induced vitamin D may be involved: a possible role of sun-induced Vitamin D. The Journal of steroid biochemistry and molecular biology. 2007; 103:675-678. [PubMed: 17229569]
- Tang JY, Parimi N, Wu A, Boscardin WJ, Shikany JM, Chren MM, Cummings SR, Epstein EH Jr. Bauer DC, G. Osteoporotic Fractures in Men Study. Inverse association between serum 25(OH) vitamin D levels and non-melanoma skin cancer in elderly men. Cancer causes & control : CCC. 2010; 21:387-391. [PubMed: 19921445]
- Bikle DD. Vitamin D: an ancient hormone. Experimental dermatology. 2011; 20:7-13. [PubMed: 21197695]
- Godar DE, Landry RJ, Lucas AD. Increased UVA exposures and decreased cutaneous Vitamin D(3) levels may be responsible for the increasing incidence of melanoma. Medical hypotheses. 2009; 72:434-443. [PubMed: 19155143]
- Yokomura K, Suda T, Sasaki S, Inui N, Chida K, Nakamura H. Increased expression of the 25- hydroxyvitamin D(3)-1alpha-hydroxylase gene in alveolar macrophages of patients with lung cancer. The Journal of clinical endocrinology and metabolism. 2003; 88:5704-5709. [PubMed: 14671156]
- Porojnicu AC, Robsahm TE, Dahlback A, Berg JP, Christiani D, Bruland OS, Moan J. Seasonal and geographical variations in lung cancer prognosis in Norway. Does Vitamin D from the sun play a role? Lung cancer. 2007; 55:263-270. [PubMed: 17207891]
- Ramirez AM, Wongtrakool C, Welch T, Steinmeyer A, Zugel U, Roman J. Vitamin D inhibition of pro-fibrotic effects of transforming growth factor beta1 in lung fibroblasts and epithelial cells. The Journal of steroid biochemistry and molecular biology. 2010; 118:142-150. [PubMed: 19931390]
- Chen TC, Holick MF. Vitamin D and prostate cancer prevention and treatment. Trends in endocrinology and metabolism: TEM. 2003; 14:423-430. [PubMed: 14580762]
- Shannan B, Seifert M, Boothman DA, Tilgen W, Reichrath J. Clusterin over-expression modulates proapoptotic and antiproliferative effects of 1,25(OH)2D3 in prostate cancer cells in vitro. The Journal of steroid biochemistry and molecular biology. 2007; 103:721-725. [PubMed: 17224269]
- Perez-Lopez FR, Chedraui P, Haya J. Review article: vitamin D acquisition and breast cancer risk. Reproductive sciences. 2009; 16:7-19. [PubMed: 19144887]
- Segaert S, Degreef H, Bouillon R. Vitamin D receptor expression is linked to cell cycle control in normal human keratinocytes. Biochemical and biophysical research communications. 2000; 279:89-94. [PubMed: 11112422]
- Kramer C, Seltmann H, Seifert M, Tilgen W, Zouboulis CC, Reichrath J. Characterization of the vitamin D endocrine system in human sebocytes in vitro. The Journal of steroid biochemistry and molecular biology. 2009; 113:9-16. [PubMed: 19027855]
- Bikle DD, Oda Y, Xie Z. Vitamin D and skin cancer: a problem in gene regulation. The Journal of steroid biochemistry and molecular biology. 2005; 97:83-91. [PubMed: 16039846]
- Christakos S, Dhawan P, Liu Y, Peng X, Porta A. New insights into the mechanisms of vitamin D action. Journal of cellular biochemistry. 2003; 88:695-705. [PubMed: 12577303]
- Lips P. Vitamin D physiology. Progress in biophysics and molecular biology. 2006; 92:4-8. [PubMed: 16563471]
- Haussler MR, Jurutka PW, Mizwicki M, Norman AW. Vitamin D receptor (VDR)-mediated actions of 1alpha,25(OH)(2)vitamin D(3): genomic and non-genomic mechanisms. Best practice & research. Clinical endocrinology & metabolism. 2011; 25:543-559. [PubMed: 21872797]
- Dixon KM, Deo SS, Norman AW, Bishop JE, Halliday GM, Reeve VE, Mason RS. In vivo relevance for photoprotection by the vitamin D rapid response pathway. The Journal of steroid biochemistry and molecular biology. 2007; 103:451-456. [PubMed: 17223553]
- Mason RS, Sequeira VB, Dixon KM, Gordon-Thomson C, Pobre K, Dilley A, Mizwicki MT, Norman AW, Feldman D, Halliday GM, Reeve VE. Photoprotection by 1alpha,25- dihydroxyvitamin D and analogs: further studies on mechanisms and implications for UV-damage. The Journal of steroid biochemistry and molecular biology. 2010; 121:164-168. [PubMed: 20399269]
- Osborne JE, Hutchinson PE. Vitamin D and systemic cancer: is this relevant to malignant melanoma? The British journal of dermatology. 2002; 147:197-213. [PubMed: 12174089]
- Colston K, Colston MJ, Feldman D. 1,25-dihydroxyvitamin D3 and malignant melanoma: the presence of receptors and inhibition of cell growth in culture. Endocrinology. 1981; 108:10831086. [PubMed: 6257495]
- Frampton RJ, Omond SA, Eisman JA. Inhibition of human cancer cell growth by 1,25- dihydroxyvitamin D3 metabolites. Cancer research. 1983; 43:4443-4447. [PubMed: 6307514]
- Evans SR, Houghton AM, Schumaker L, Brenner RV, Buras RR, Davoodi F, Nauta RJ, Shabahang M. Vitamin D receptor and growth inhibition by 1,25-dihydroxyvitamin D3 in human malignant melanoma cell lines. The Journal of surgical research. 1996; 61:127-133. [PubMed: 8769954]
- Eisman JA, Barkla DH, Tutton PJ. Suppression of in vivo growth of human cancer solid tumor xenografts by 1,25-dihydroxyvitamin D3. Cancer research. 1987; 47:21-25. [PubMed: 3024816]
- Danielsson C, Fehsel K, Polly P, Carlberg C. Differential apoptotic response of human melanoma cells to 1 alpha,25-dihydroxyvitamin D3 and its analogues. Cell death and differentiation. 1998; 5:946-952. [PubMed: 9846181]
- Field S, Newton-Bishop JA. Melanoma and vitamin D. Molecular oncology. 2011; 5:197-214. [PubMed: 21371954]
- Nurnberg B, Graber S, Gartner B, Geisel J, Pfohler C, Schadendorf D, Tilgen W, Reichrath J. Reduced serum 25-hydroxyvitamin D levels in stage IV melanoma patients. Anticancer research. 2009; 29:3669-3674. [PubMed: 19667163]
- Brozyna AA, Jozwicki W, Janjetovic Z, Slominski AT. Expression of vitamin D receptor decreases during progression of pigmented skin lesions. Human pathology. 2011; 42:618-631. [PubMed: 21292298]
- Brozyna AA, Jozwicki W, Janjetovic Z, Slominski AT. Expression of the vitamin D-activating enzyme 1alpha-hydroxylase (CYP27B1) decreases during melanoma progression. Human pathology. 2013; 44:374-387. [PubMed: 22995334]
- Brozyna AA, Jozwicki W, Slominski AT. Decreased VDR expression in cutaneous melanomas as marker of tumor progression: new data and analyses. Anticancer research. 2014; 34:2735-2743. [PubMed: 24922634]
- Tang JY, Fu T, Leblanc E, Manson JE, Feldman D, Linos E, Vitolins MZ, Zeitouni NC, Larson J, Stefanick ML. Calcium plus vitamin D supplementation and the risk of nonmelanoma and melanoma skin cancer: post hoc analyses of the women's health initiative randomized controlled trial. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 2011; 29:3078-3084. [PubMed: 21709199]
- Denzer N, Vogt T, Reichrath J. Vitamin D receptor (VDR) polymorphisms and skin cancer: A systematic review. Dermato-endocrinology. 2011; 3:205-210. [PubMed: 22110781]
- Hustmyer FG, DeLuca HF, Peacock M. Apal, BsmI, EcoRV and TaqI polymorphisms at the human vitamin D receptor gene locus in Caucasians, blacks and Asians. Human molecular genetics. 1993; 2:487. [PubMed: 8099305]
- Morrison NA, Qi JC, Tokita A, Kelly PJ, Crofts L, Nguyen TV, Sambrook PN, Eisman JA. Prediction of bone density from vitamin D receptor alleles. Nature. 1994; 367:284-287. [PubMed: 8161378]
- Ma J, Stampfer MJ, Gann PH, Hough HL, Giovannucci E, Kelsey KT, Hennekens CH, Hunter DJ. Vitamin D receptor polymorphisms, circulating vitamin D metabolites, and risk of prostate cancer in United States physicians. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 1998; 7:385-390.
- Morrison NA, Yeoman R, Kelly PJ, Eisman JA. Contribution of trans-acting factor alleles to normal physiological variability: vitamin D receptor gene polymorphism and circulating osteocalcin. Proceedings of the National Academy of Sciences of the United States of America. 1992; 89:6665-6669. [PubMed: 1353882]
- Jurutka PW, Whitfield GK, Hsieh JC, Thompson PD, Haussler CA, Haussler MR. Molecular nature of the vitamin D receptor and its role in regulation of gene expression. Reviews in endocrine & metabolic disorders. 2001; 2:203-216. [PubMed: 11705326]
- Faraco JH, Morrison NA, Baker A, Shine J, Frossard PM. Apal dimorphism at the human vitamin D receptor gene locus. Nucleic acids research. 1989; 17:2150. [PubMed: 2564666]
- Gross C, Eccleshall TR, Malloy PJ, Villa ML, Marcus R, Feldman D. The presence of a polymorphism at the translation initiation site of the vitamin D receptor gene is associated with low bone mineral density in postmenopausal Mexican-American women. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 1996; 11:1850-1855.
- Saijo T, Ito M, Takeda E, Huq AH, Naito E, Yokota I, Sone T, Pike JW, Kuroda Y. A unique mutation in the vitamin D receptor gene in three Japanese patients with vitamin D-dependent rickets type II: utility of single-strand conformation polymorphism analysis for heterozygous carrier detection. American journal of human genetics. 1991; 49:668-673. [PubMed: 1652893]
- Arai H, Miyamoto K, Taketani Y, Yamamoto H, Iemori Y, Morita K, Tonai T, Nishisho T, Mori S, Takeda E. A vitamin D receptor gene polymorphism in the translation initiation codon: effect on protein activity and relation to bone mineral density in Japanese women. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 1997; 12:915-921.
- Whitfield, GK.; Remus, LS.; Jurutka, PW.; Zitzer, H.; Oza, AK.; Hope, T. Are human vitamin D receptor polymorphisms functionally significant?. In: Norman, AW.; Bouillon, R.; Thomasset, M., editors. Vitamin D Endocrine system, Structural, Biological, Genetic and Clinical Aspects. University of California; Riverside, CA.: 2000. p. 817-823.
- Jurutka PW, Remus LS, Whitfield GK, Thompson PD, Hsieh JC, Zitzer H, Tavakkoli P, Galligan MA, Dang HT, Haussler CA, Haussler MR. The polymorphic N terminus in human vitamin D receptor isoforms influences transcriptional activity by modulating interaction with transcription factor IIB. Molecular endocrinology. 2000; 14:401-420. [PubMed: 10707958]
- Colin EM, Weel AE, Uitterlinden AG, Buurman CJ, Birkenhager JC, Pols HA, van Leeuwen JP. Consequences of vitamin D receptor gene polymorphisms for growth inhibition of cultured human peripheral blood mononuclear cells by 1, 25-dihydroxyvitamin D3. Clinical endocrinology. 2000; 52:211-216. [PubMed: 10671949]
- Carless MA, Kraska T, Lintell N, Neale RE, Green AC, Griffiths LR. Polymorphisms of the VDR gene are associated with presence of solar keratoses on the skin. The British journal of dermatology. 2008; 159:804-810. [PubMed: 18647306]
- Zhao XZ, Yang BH, Yu GH, Liu SZ, Yuan ZY. Polymorphisms in the vitamin D receptor (VDR) genes and skin cancer risk in European population: a meta-analysis. Archives of dermatological research. 2014; 306:545-553. [PubMed: 24771013]
- Jorgensen TJ, Ruczinski I, Yao Shugart Y, Wheless L, Berthier Schaad Y, Kessing B, Hoffman- Bolton J, Helzlsouer KJ, Kao WH, Francis L, Alani RM, Strickland PT, Smith MW, Alberg AJ.
- A population-based study of hedgehog pathway gene variants in relation to the dual risk of basal cell carcinoma plus another cancer. Cancer epidemiology. 2012; 36:e288-293. [PubMed: 22677152]
- Lesiak A, Norval M, Wodz-Naskiewicz K, Pawliczak R, Rogowski-Tylman M, Sysa- Jedrzejowska A, Sobjanek M, Wlodarkiewicz A, Narbutt J. An enhanced risk of basal cell carcinoma is associated with particular polymorphisms in the VDR and MTHFR genes. Experimental dermatology. 2011; 20:800-804. [PubMed: 21732987]
- Han J, Colditz GA, Hunter DJ. Polymorphisms in the MTHFR and VDR genes and skin cancer risk. Carcinogenesis. 2007; 28:390-397. [PubMed: 16950800]
- Schafer A, Emmert S, Kruppa J, Schubert S, Tzvetkov M, Mossner R, Reich K, Berking C, Volkenandt M, Pfohler C, Schon MP, Vogt T, Konig IR, Reichrath J. No association of vitamin D metabolism-related polymorphisms and melanoma risk as well as melanoma prognosis: a case- control study. Archives of dermatological research. 2012; 304:353-361. [PubMed: 22576141]
- Orlow I, Roy P, Reiner AS, Yoo S, Patel H, Paine S, Armstrong BK, Kricker A, Marrett LD, Millikan RC, Thomas NE, Gruber SB, Anton-Culver H, Rosso S, Gallagher RP, Dwyer T, Kanetsky PA, Busam K, From L, Begg CB, Berwick M, Group GEMS. Vitamin D receptor polymorphisms in patients with cutaneous melanoma. International journal of cancer. Journal international du cancer. 2012; 130:405-418. [PubMed: 21365644]
- Mandelcorn-Monson R, Marrett L, Kricker A, Armstrong BK, Orlow I, Goumas C, Paine S, Rosso S, Thomas N, Millikan RC, Pole JD, Cotignola J, Rosen C, Kanetsky PA, Lee-Taylor J, Begg CB, Berwick M. Sun exposure, vitamin D receptor polymorphisms FokI and BsmI and risk of multiple primary melanoma. Cancer epidemiology. 2011; 35:e105-110. [PubMed: 21612999]
- Mocellin S, Nitti D. Vitamin D receptor polymorphisms and the risk of cutaneous melanoma: a systematic review and meta-analysis. Cancer. 2008; 113:2398-2407. [PubMed: 18816636]
- Santonocito C, Capizzi R, Concolino P, Lavieri MM, Paradisi A, Gentileschi S, Torti E, Rutella S, Rocchetti S, Di Carlo A, Di Stasio E, Ameglio F, Zuppi C, Capoluongo E. Association between cutaneous melanoma, Breslow thickness and vitamin D receptor BsmI polymorphism. The British journal of dermatology. 2007; 156:277-282. [PubMed: 17223867]
- Slominski A, Janjetovic Z, Tuckey RC, Nguyen MN, Bhattacharya KG, Wang J, Li W, Jiao Y, Gu W, Brown M, Postlethwaite AE. 20S-hydroxyvitamin D3, noncalcemic product of CYP11A1 action on vitamin D3, exhibits potent antifibrogenic activity in vivo. The Journal of clinical endocrinology and metabolism. 2013; 98:E298-303. [PubMed: 23295467]
- Slominski A, Kim TK, Zmijewski MA, Janjetovic Z, Li W, Chen J, Kusniatsova EI, Semak I, Postlethwaite A, Miller DD, Zjawiony JK, Tuckey RC. Novel vitamin D photoproducts and their precursors in the skin. Dermato-endocrinology. 2013; 5:7-19. [PubMed: 24494038]
- Slominski AT, Janjetovic Z, Fuller BE, Zmijewski MA, Tuckey RC, Nguyen MN, Sweatman T, Li W, Zjawiony J, Miller D, Chen TC, Lozanski G, Holick MF. Products of vitamin D3 or 7- dehydrocholesterol metabolism by cytochrome P450scc show anti-leukemia effects, having low or absent calcemic activity. PloS one. 2010; 5:e9907. [PubMed: 20360850]
- Slominski AT, Carlson JA. Melanoma resistance: a bright future for academicians and a challenge for patient advocates. Mayo Clinic proceedings. 2014; 89:429-433. [PubMed: 24684870]