Toggle Health Problems and D

Vitamin D from animal products - July 2013

Natural Vitamin D Content in Animal Products

Advances in Nutrition July 2013 vol. 4: 453-462, 2013  
Alexandra Schmid and Barbara Walther
PDF is attached at the bottom of this page

Note by VitaminDWiki

Tuna liver has a LOT of vitamin D 32,500 micrograms per kg = 1300 IU/gram = 37,000 IU/ounce
Tilapia might have more vitamin D than wild salmon, but has very high level of Omega 6 (bad)

See also VitaminDWiki

See also web

Tables are followed by text - which has interesting comments on cooking, etc.

Table 1 raw meat and offal

Koshy and VanDerSlik (25)Cow, liver 2.7–5.3
 Cow, muscle 1.5–3.4
 Cow, kidney 5.1–9.8
Mattila et al. (26)Beef, steak<0.50.8
 Beef, chuck<0.50.5
 Beef, liver<0.53.4
Kobayashi et al. (27)Beef (separable lean)0 
 Beef, liver0 
Montgomery et al. (30)Beef, top round steak2.81.3
 Beef, strip loin steak4.11.4
 Beef, liver8.67.7
 Beef, kidney7.423.3
Montgomery et al. (29)Beef, strip loin steaks9.54.1
 Beef, liver140.81.9
Wertz et al. (28)Beef, longissimus muscle 0.9
Foote et al. (33)Beef, longissimus dorsi muscle1.11.7
 Beef, semimembranosus muscle0.81.4
 Beef, infraspinatus muscle1.10.9
 Beef, liver1.92.6
 Beef, kidney1.33.0
Montgomery et al. (32)Beef, longissimus muscle5.30.3
 Beef, liver13.30.8
 Beef, kidney12.21.6
Montgomery et al. (31)Beef, strip loin2.6–10.00.2–0.4
 Beef, liver12.3–14.20.7–0.9
 Beef, kidney4.2–27.10.9–2.0
Purchas et al. (34)Beef, shoulder roast1.04.8
 Beef, rump mince1.35.8
 Beef, strip loin1.12.7
 Beer, topside roast0.94.8
Mattila et al. (26)Pork, fillet1.1<0.6
 Pork, Boston butt3.40.7
 Pork, liver4.04.4
Kobayashi et al. (27)Pork (separable lean)7.0 
 Pork (total edible)13.8 
 Pork, liver12.5 
Clausen et al. (35)Pork, loin1.50.9
 Pork, leg inside0.50.7
 Pork, thin belly2.11.4
 Pork, neck1.61.3
Wilborn et al. (36)Pork, longissimus muscle13.969.3
Bilodeau et al. (37)Pork, lean ground2.10.9
 Pork, medium ground2.40.9
 Pork, center chops boneless (whole)1.70.8
 Pork, tenderloin (whole)1.81.4
 Pork, back ribs (whole)3.91.3
 Pork, shoulder blade roast boneless (whole)3.71.2
 Pork, loin rib roast (whole)3.11.0
Kobayashi et al. (27)Sheep (mutton and lamb)0 
Purchas et al. (34)Lamb, leg roast0.612
 Lamb, leg steak0.410.4
 Lamb, rack roast0.35.7
 Lamb, shoulder chop0.98.4
Kobayashi et al. (27)Chicken, breast0 
 Chicken, liver2.0 
 Domestic duck23.0 
Mattila et al. (26)Chicken2.92.5
Mattila et al. (38)Chicken, leg and thigh3.0≤2.0
 Chicken, fillet2.0≤2.0

Table 2 in milk and dairy products

Mouillet et al. (45)Raw milk0.60–1.38   
Kunz et al. (46)Cow’s milk0.050.50  
Takeuchi et al. (47)Cow’s milk0.420.27  
Kneifel (48)Raw milk0.1–2.0   
 Whole milk PAST0.8   
 UHT milk0.8   
Mattila et al. (26)Milk<0.2< 0.2  
Trenerry et al. (49)Milk (0.1% fat)<0.02   
 Milk (1.0% fat)<0.02   
 Whole milk (3.8% fat)0.02   
 Raw milk (4.5% fat)0.06   
Hollis et al. (57)Raw milk0.040.37  
McDermot et al. (58)Raw milk0.0750.250  
Jakobsen and Saxholt (50)Whole milk0.0920.0750.0340.031
 Milk semiskimmed0.0460.042  
 Whole milk (organic)0.0760.057  
Takeuchi et al. (63)Butter7.25   
Kneifel (48)Butter10   
Mattila et al. (26)Butter2.00.50.5 
Jakobsen and Saxholt (50)Butter1.960.960.610.58
Kneifel (48)Cheese (curd, Camembert, Edam, Gouda, Emmental)0–10   
Mattila et al. (26)Edam cheese1.10.5  
Mattila et al. (26)Cream0.70.90.1 
Jakobsen and Saxholt (50)Cream0.940.59  
Jakobsen and Saxholt (50)Coffee cream0.440.27  
  • 1 All values are means in μg/L unless indicated otherwise. 25(OH)-D-3, 25-hydroxycholecalciferol; 25(OH)-D2, 25-hydroxyergocalciferol; UHT, ultra heat-treated.
  • 2 Unclear whether vitamins D-3 + D-2 or D-3 or D-2.
  • 3 Cows supplemented with 4000 iu/d.
  • 4 All values for butter and cheese are means in μ/kg.

Table 3 chicken eggs

Koshy and VanDerSlik (70)Egg, yolk 5.0–8.0
Jackson et al. (68)Egg, whole16 
Sivell et al. (69)Egg, whole8–14 
Takeuchi et al. (63)Egg, yolk39 
Mattila et al. (66)Egg, yolk (autumn)40 
 Egg, yolk (spring)56 
Mattila et al. (65)Egg, yolk 9.8
Kobayashi et al. (27)Egg, whole30 
 Egg, yolk58 
Mattila et al. (67)Egg, yolk349.3
Mattila et al. (38)Egg, whole143.8
 Egg, yolk (autumn)4010.0
 Egg, yolk (spring)4913.0
  • 1 Mean values. 25(OH)-D-3, 25-hydroxycholecalciferol.
  • 2 Sum of vitamins D-3 and D-2.

Table 4  fish

Egaas and Lambertsen (89)Tuna, liver32,500 
 Mackerel, liver2400 
 Mackerel, fillet155 
 Mackerel, red muscle155 
 Coalfish, liver165 
Takeuchi et al. (63)Japanese pilchard1361 
 Tuna, fatty meat37 
Kobayashi et al. (27)Anglerfish, liver1100 
 Indo-Pacific blue marlin350 
 Chum salmon325 
 Flat fish230 
 Bastard halibut (cultured)180 
 Bluefin tuna, fatty meat180 
 Sand eel150 
 Rainbow trout150 
 Red sea bream (cultured)130 
 Pacific saury110 
 Japanese pilchard98 
Mattila et al. (72)Baltic herring171.0ND
 Rainbow trout (cultured)76.01.4
Lu et al. (74)Wild salmon249.0 
 Farmed salmon60.5 
 Blue fish70.6 
 Farmed trout97.8 
 Tuna ahi101.8 
Bilodeau et al. (37)Mahi mahi11.1<0.2
 Canned pink salmon223.01.1
  • 1 Mean values. 25(OH)-D-3, 25-hydroxycholecalciferol; ND, not detected.
  • 2 Sum of vitamins D-3 and D-2.


Humans derive most vitamin D from the action of sunlight in their skin. However, in view of the current Western lifestyle with most daily activities taking place indoors, sun exposure is often not sufficient for adequate vitamin D production. For this reason, dietary intake is also of great importance. Animal foodstuffs (e.g., fish, meat, offal, egg, dairy) are the main sources for naturally occurring cholecalciferol (vitamin D-3). This paper therefore aims to provide an up-to-date overview of vitamin D-3 content in various animal foods. The focus lies on the natural vitamin D-3 content because there are many countries in which foods are not regularly fortified with vitamin D.The published data show that the highest values of vitamin D are found in fish and especially in fish liver, but offal also provides considerable amounts of vitamin D. The content in muscle meat is generally much lower. Vitamin D concentrations in egg yolks range between the values for meat and offal. If milk and dairy products are not fortified, they are normally low in vitamin D, with the exception of butter because of its high fat content. However, as recommendations for vitamin D intake have recently been increased considerably, it is difficult to cover the requirements solely by foodstuffs. Adv. Nutr. 4: 453-462, 2013.


The term vitamin D, as used here, refers to the secosteroids ergocalciferol (vitamin D-2) and cholecalciferol (vitamin D-3). Their structural difference lies only in the C-17 side chain, the former having an additional C-22 to C-23 double bond and a C-24 methyl group (Fig. 1). Vitamin D-2 is formed by UV radiation (in particular UVB radiation) of ergosterol, which is found in plants, fungi, and invertebrates. For vitamin D-3, the genesis is the same, but the provitamin is 7-dehydrocholesterol, which is found in vertebrates. In humans as well, vitamin D-3 is synthesized in the skin with the help of sunlight (1).
After the production of vitamin D-3 in the skin or after its absorption from the gut, the vitamin is transported to the liver, bound in blood to vitamin D-binding protein. In the liver, it is hydroxylated to 25-hydroxycholecalciferol [25(OH)-D-3] (calcidiol), the rate being related to substrate supply (2). 25(OH) D3 is the major circulating metabolite of vitamin D, and its concentration in blood is used as a measure of vitamin D status (1,3,4). In the kidney, 25(OH)D is activated with a second hydroxylation to 1,25-dihydroxycholecalciferol [1,25(OH)2-D-3] (calcitriol), the production being tightly regulated by calcium (via parathyroid hormone), by phosphate (via fibroblast growth factor 23), and during growth and pregnancy (1,5,6). The main target tissues of 1,25(OH)2-D-3 are intestine, bone, and kidney where it carries out hormonal functions. 25(OH)-D-3 as well as 1,25(OH)2-D-3 are catabolized and the final products are excreted in the bile(2).
Humans derive most vitamin D from the action of sunlight in their skin. However, the current Western lifestyle with most activities taking place indoors often prevents sufficient sun expose. In addition, season and latitude may diminish the intensity of the sun, and also clothing, sunscreen, and skin pigmentation interfere with vitamin D synthesis (6). In this case, dietary intake becomes increasingly important. However, only a few foodstuffs, mainly of animal origin (e.g., fish, meat, offal, egg, dairy), are recommended in literature to be a valuable source of naturally occurring vitamin D-3 (2). This report compiles the available information on vitamin D content in various animal foods in relation to the prevention of vitamin Ddeficiency. The focus is on the natural vitamin D-3 content because there are many countries (e.g., most of the European countries) in which foods are not regularly fortified with vitamin D. 25-Hydroxycholecalciferol is included because this metabolite contributes to the total biological activity (7).

Functions of vitamin D

Figure 1 Structure of ergocalciferol (top) and cholecalciferol (bottom).

The vitamin D metabolite 1,25(OH)2-D-3 acts as a hormone in the regulation of calcium and phosphorus metabolism with the aim of maintaining normal calcium and phosphorus concentrations in serum ensuring a normal mineralization of bone (1,2). Vitamin D may also play a role in muscle development because vitamin D receptor is located in muscle tissue and vitamin D deficiency leads to muscle weakness. Several further tissues exhibit a vitamin D receptor, among other brain, prostate, breast, and colon tissues as well as immune cells. Epidemiologic findings suggest benefits of vitamin D on cardiovascular mortality, hypertension, colorectal cancer, multiple sclerosis, type 1 diabetes, immune function, and inflammation (1,8). However, apart from the positive influence of vitamin D on fracture risk and falls, data are not yet compelling but need further evaluation in clinical studies.

Requirements of vitamin D and vitamin D deficiency

25(OH)-D-3 concentrations in blood are used to determine the vitamin D status (9). Serum levels of <25 nmol/L come along with the risk of rickets (deformation of bones due to inadequate development) in children and of osteomalacia (brittle bones) in adults. Levels of 25 to 49 nmol/L are seen as insufficient (10). The question about the optimal serum concentration cannot yet be answered clearly. In adults, data suggest that serum levels of >75 nmol/L are optimal for bone health as well as for nonskeletal health benefits of vitamin D. However, concentrations between 50 and 60 nmol/L are often seen as (more conservative) target because above that there is no further increase in bone density, muscle function, and parathyroid hormone suppression (6,11).
With sufficient UVB exposure, dietary intake of vitamin D is not necessary. However, due to environmental influences (see above) synthesis of vitamin D is not always adequate; therefore, dietary intake is advisable. The RDA of vitamin D for females and males between 9 and 70 y of age in the United States is set at 15 ug/d (600 IU/d) and also the Endocrine Society suggests this amount for adults aged 19-50 y but indicates that to increase the blood level of 25(OH)-D-3 consistently above 30 ug/L, at least 37.550.0 ug/d (1500-2000 IU/d) of vitamin D may be required. The German-speaking nutrition societies (D-A-CH recommendations) recently increased their recommendation for children and adults from 5 ug/d (200 IU/d) to 20 ug/d (800 IU/d), whereas the European RDA for adults remains at a daily intake of 5 ug/d (200 IU/d), but the upper limit from 50 ug/d (2000 IU/d) was increased to 100 ug/d (4000 IU/d) (11-15).

Vitamin D in animal food

Only a few foodstuffs naturally contain vitamin D, and these foodstuffs are mainly of animal origin. Above all, vitamin D-3 is found in these products together with its metabolite 25(OH)-D-3. As the latter is also biologically active and such contributes to dietary intake, it is included in the following compilation (7). There is still no consensus, if compared with vitamin D-3, that the bioactivity of 25(OH)-D-3 is higher. A bioactivity up to 5 times higher is proposed, but could not be demonstrated in all investigations (16). However, the outcome of a recent study supports a factor of 45 (17). Other metabolites are only contained in trace amounts and do not contribute much to biological vitamin D activity (7). In dairy, varying amounts of vitamin D-2 and 25-hydroxyergocalciferol [25(OH)-D-2] are documented and therefore are also mentioned in the overview. However, supplementation studies suggest that vitamin D-2 is not as potent as vitamin D-3 in increasing serum 25-hydroxyvitamin D [25(OH)-D] concentrations (18). Described below are results of studies determining the vitamin D content by HPLC or liquid chromatography-linear mass spectrometry (LC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. Investigations based on classic bioassays measuring antirachitic activity are not included in this overview.

Meat, offal, and meat products

A search of the national food composition databases of Denmark, France, Germany, Switzerland, Canada, and the United States for vitamin D content in various raw meat cuts yielded the following values: 0.0-9.0 ug/kg for beef, 1.0-23.0 ug/kg for pork, 1.0-61.0 ug/kg for lamb, 0.050.0 ug/kg for veal, 0.0-14.0 ug/kg for poultry, and 0.023.0 ug/kg for various meat products (19-24). However, the vitamin D content is not available for all meat cuts, and the databases do not give any information about how the data were obtained. A few values are inexplicably high and may be based on a single analysis and/or that the analyzed meat originated from animals supplemented with vitamin D.
Table 1 presents vitamin D-3 and 25(OH)-D-3 content of meat and offal found in literature. Data on animals whose diets were supplemented beyond the normal with vitamin D-3 or 25(OH)-D-3 were excluded. Koshy and VanDerSlik (25) examined 25(OH)-D-3 contents in raw liver, muscle, and kidney from cows in Michigan. The highest concentrations o

Table 1. Published natural vitamin D-3 and 25(OH)-D-3 content in raw meat and offal1

were found in kidney and the lowest in muscle meat. Mattila et al. (26) investigated raw beef meat and liver samples that were purchased in retail stores in the Helsinki area in autumn and spring. Vitamin D-3 concentrations were <0.5 ug/kg in all beef samples; 25(OH)-D-3 concentrations ranged from <0.5 to 3.5 ug/kg, with the highest values in liver. The results did not vary substantially between the 2 seasons. Kobayashi et al. (27) did not find any vitamin D (sum of vitamins D-3 and D-2) in bovine meat and offal when analyzing 69 different Japanese foods purchased from markets. Between 2000 and 2004, several experiments of the Department of Animal Science at the Iowa State University regarding the effect of vitamin D-3 supplementation on beef tenderness were published (28-33). The vitamin D-3 concentrations in the control groups of the steers (receiving 90% concentrate diet with a commercial nutrient supplement) ranged between 0.8 and 10.0 ug/kg in raw meat, between 1.9 and 140.8 ug/kg in raw liver, and between 1.3 and 27.1 ug/kg in raw kidney. For 25(OH)-D-3, the concentrations in meat were0.2-4.1 ug/kg, in liver 0.7-7.7 ug/kg, and in kidney 0.9-23.3 ug/kg. Muscle concentrations of vitamin D-3 and 25(OH)-D-3 vary significantly according to biological type of cattle, liver concentrations, however, do not (31). Additional vitamin D-3 supplementations up to 7.5 million IU/steer for 8 or 9 days before slaughter increased vitamin D-3 and 25(OH)-D-3 values in meat and offal (29,30,32,33). Purchas et al. (34) found vitamin D-3 concentrations between 0.9 and 1.3 ug/kg and 25(OH)-D-3 concentrations between 2.7 and 5.8 ug/kg in raw beef meat (various cuts) of animals raised on pasture without any supplements.
Analyses by Mattila et al. (26) in raw pork meat purchased in several retail stores and pork liver bought from 1 meat wholesaler in the Helsinki area in spring and autumn showed only minor variations in the results of the 2 seasons. In the case of the muscle samples, a positive correlation between the fat and vitamin D-3 content was found. Compared with Mattila et al. (26), Kobayashi et al. (27) found rather high vitamin D (sum of vitamins D-3 and D-2) amounts in meat and liver of pork, respectively. In raw pork cuts of varying fat content, Clausen et al. (35) measured vitamin D-3 concentrations from 0.5 to 2.1 ug/kg and 25(OH)-D-3 concentrations from 0.7 to 1.4 ug/kg. Vitamin D-3 and 25(OH)-D-3 were significantly associated with the fat content of whole cuts, and in the cuts 8 to 10 times more vitamin D-3 and 2 to 3 times more 25(OH)-D-3 was found in lard and intramuscular fat than in the lean parts. Wilborn et al. (36) investigated the effect of supplemental vitamin D on pork quality. In the control group, vitamin D-3 and 25(OH)-D-3 concentrations in longissimus muscle were 13.9 and 69.3 ug/kg, respectively, which is substantially higher than the values given in the other publications. Last, but not least, Bilodeau et al. (37) determined vitamin D-3 and 25(OH)-D-3 concentrations in various raw pork cuts collected from major retail centers in Canada. Vitamin D-3 concentrations ranged from 0.8 to 4.2 ug/kg and 25(OH)-D-3 concentrations from 0.8 to 1.4 ug/kg.
In raw samples from 4 lamb cuts (lambs raised on pasture), Purchas et al. (34) determined vitamin D-3 concentrations of 0.3-0.9 ug/kg and 25(OH)-D-3 concentrations of 5.7-12.0 ug/kg. By contrast, Kobayashi et al. (27) found no vitamin D in lamb and mutton.
Chicken samples purchased in spring and autumn from retail stores in Finland were analyzed for their vitamin D-3 and 25(OH)-D-3 content by Mattila et al. (26). Mean vitamin D-3 content was 2.9 ug/kg and 25(OH)-D-3 2.5 ug/kg with no significant seasonal difference. In a more recent repetition of this investigation similar vitamin D-3 concentrations were detected, but 25(OH)-D-3 concentration was below detection limit (38). Kobayashi et al. (27) found no vitamin D in chicken breast but slight amounts in chicken liver and turkey and a high amount (325 ug/kg) in domestic duck. The latter might be due to supplements in feedstuff.
In meat products, the vitamin D content is dependent on the vitamin D concentration of the processed fresh meat and the fat content. In various Swiss meat products, vitamin D-3 content from below detection limit (<2.5 ug/kg) to 23 ug/kg was found (39-42). Values below detection limit were, for instance, found in different types of cooked ham and in dried beef with little fat. The largest amounts provided salami type meat products, Vienna sausages, and lard.


A search in different national food composition databases of Denmark, France, Germany, Switzerland, Canada, and the United States for vitamin D content in dairy products yielded the following values: whole milk, 0.3-1.0 ug/kg (US and Canada present only values for fortified whole milk: 7.05 ug/kg and 9.9 ug/kg, respectively); cream, 3.710.8 ug/kg; butter, 5.9-14.1 ug/kg; yogurt, 0.4-6.0 ug/kg; curd cheese, 2.0-7.05 ug/kg; soft cheese, 2.8-5.8 ug/kg; semihard and hard cheese, 2.0-18.1 ug/kg. Only few data are available in these databases for dairy products from non-cow origin: goat's milk, 0.6-2.8 ug/kg; ewe's milk, 1.8 ug/kg; feta, 3.0-4.0 ug/kg. The databases do not differentiate between vitamins D-2 and D-3, nor give any information about inclusion or exclusion of the vitamin D metabolites and how the data were obtained (19-24).
The influence of different factors like fodder, supplementation, sunlight, seasons, and races on the vitamin D content in (cow's) milk and dairy products has been a topic for >80 y (43). Modern analytical methods like HPLC, LC-MS, and LC-MS/MS confirmed the results from earlier studies obtained by indirect detection by biological assays (44). On the other hand, they also opened the possibility to differentiate between the several metabolites and to give more quantified results (37). In both periods, the findings agree that vitamin D content in milk and dairy products is originally rather low. Table 2 presents vitamin D-3 and 25(OH)-D-3 content of milk and dairy products found in literature. However, the detected values partly vary considerably among the different studies (7,44).

Table 2.

Animal supplementation influences significantly the concentration of vitamin D in milk, which explains the relatively high values found in the milk of these animals. Data of animals whose diets were supplemented with vitamin D-3 or 25(OH)-D-3 were therefore excluded. On the other hand, in milk and dairy products vitamin D-2 and 25(OH)-D2 are partially found in relevant concentrations, so we decided to include these values in Table 2 where available. Mouillet et al. (45) collected raw milk samples from different regions in France. The concentration of vitamin D-3 varied between and within the regions. Kunz et al. (46) distinguish between the various metabolites and found 10 times more 25(OH)-D than vitamin D, but they give no information on whether the values are the sum of vitamins D-2 and D-3 or only 1 of them. In commercially available milk samples in Japan, 0.42 ug/L vitamin D-3 and 0.27 ug/L 25(OH)D3, but neither vitamin D-2 nor its metabolites were detected (47). In his review, Kneifel (48) listed a range of 0.1-2.0 ug/L for raw milk and 0.8 ug/L for pasteurized whole milk and ultra heat-treated (UHT) milk, respectively, but it is not clear which method was used to analyze these samples. In all samples of the study carried out by Mattila et al., the values for vitamins D-2 and D-3 as well as for the metabolites were below the detection limit of 0.2 ug/L (26). Analysis with the LC-MS and LC-MS/MS method of whole milk (3.8% fat) and fresh cow's milk (~4.5% fat) detected 0.2 ug/L and 0.50.6 ug/L of vitamin D-3, respectively. The vitamin D-3 content in fat-reduced milk (0.1% and 1.0%) was below the detection limits (49). The apparent correlation of the fat-soluble vitamin with the fat content of the milk was later clearly demonstrated in a Danish study (50).
In some countries like the United States, Canada, and the United Emirates, milk and dairy products for sale are fortified with vitamin D either by law (Canada) or by choice (United States and United Emirates) (51,52). Another possibility to improve the vitamin D content in milk and dairy products is to supplement the cows. But neither the supplementation over the fodder nor the intravenous or intramuscular application of vitamin D significantly improves the vitamin D content in the milk (53-56). However, a direct oral supplementation of cows with doses from 4000 IU/d to 40,000 IU/d of vitamin D resulted in an increase of vitamin D (57), and another experiment also showed a positive correlation between increasing oral supplementation and the concentration of vitamin D in milk (58). In several countries, the oral supplementation of dairy cows is recommended with varying doses (50,59). Although this is done to secure the health of the cows and not with the aim to increase the vitamin D content in milk, it could nevertheless have an impact on it.
For some time, a water-soluble vitamin D metabolite found in whey was held responsible for an additional vitamin D activity in milk (60). But this hypothesis was not confirmed, and all vitamin D activity was explained by the sum of vitamin D-3, 24,25(OH)2-D3, 1,25(OH)2-D3, and 25(OH)-D-3 (61,62). Hollis et al. (57) realized that vitamin D and its metabolites initially present in whey fraction migrate with time into the fat fraction of the milk.
Few data are available for the vitamin D content in dairy products. Because of the high fat content, the values are significantly higher in butter than in milk; they vary from 1.96 ug/kg to 10 ug/kg (26,48,50,63). Some but not all of the authors also reported mentionable concentrations for 25(OH)-D-3, vitamin D-2, and 25(OH)-D2 (26,50). Insufficient data are available for cheese. In the list of Kneifel (48), a range of 0-10 ug/kg is given for a summary of different cheese types (curd, Camembert, Edam, Gouda, and Em-mental). More detailed are the results of the analysis of an Edam type cheese in which vitamin D-3 and 25(OH)-D-3 but not vitamin D-2 and 25(OH)-D2 were detected (26).
Cream samples collected in Finland and Denmark contained 0.7 ug/kg and 0.94 ug/kg vitamin D-3, respectively, and 0.9 ug/kg and 0.59 ug/kg 25(OH)-D-3, respectively. The Finnish research group additionally reports vitamin D-2. The amounts of vitamin D-3 and 25(OH)-D-3 in coffee cream are only half of the values for whipping cream because of the reduced fat content (26,50).
The Danish study shows significant seasonal variation of the vitamin D content in milk and dairy products, which confirms earlier observations (26,50,64).


In the above-mentioned national food composition databases, vitamin D values for the whole egg and the egg yolk in particular can be found. The vitamin D content in eggs is practically all in the yolk. Based on the whole egg, the content varies between 14.4 and 29.3 ug/kg and between 32.5 and 55.8 ug/kg for egg yolks (19-24). Table 3 shows the values found in literature. Mattila et al. (38) analyzed pools of egg yolk from commercial chicken eggs collected in the spring and autumn. Vitamin D-3 and 25(OH)-D-3 content in the egg yolk was slightly higher in spring. The values correspond to previous analyses in 1992 and 1993 (65,66); slightly lower content of vitamin D-3 and 25(OH)-D-3 was found in egg yolks by the same authors in 1999,however (67). Overall, the vitamin D-3 values found in literature vary between 8.0 and 30.0 ug/kg for whole chicken egg and between 34.0 and 58.0 ug/kg for egg yolks (27,38,66-69).

Table 3. Published natural vitamin D-3 and 25(OH)-D-3 content in chicken eggs1

The 25(OH)-D-3 is documented to range from 5.0 to 13.0 ug/ kg in egg yolks (38,65,67,70). The concentrations of vitamin D in eggs can be increased in a linear dose-dependent manner by supplementing chicken feed with vitamin D-3 (67,71).


Fish and fish products are regarded as the major dietary source of vitamin D. National food composition databases show values in the range of 0 to 300 ug/kg (19-24). Amounts found in literature are presented in Table 4.In 1984, Takeuchi et al. (63) reported the vitamin D-3 content of 8 different fish products. The values ranged between 5 and 356 ug/kg. The highest amounts were found in fresh eel and shiokara, a Japanese fish product. Kobayashi et al. (27) analyzed 18 different kinds offish and reported vitamin D (total of vitamins D-2 and D-3) concentrations from 18 to 350 ug/kg. Fish liver was as high as 1200 ug/kg of vitamin D. Various fresh fish from the Baltic sea and 2 lakes in Finland as well as frozen fish and fish products were analyzed by Mattila et al. (72). Large variations were not only found between different fish species but also in the same species caught in different locations. The observed variations in vitamin D content between fish of the same species seem not to be related to the weight, sex, or age of the fish but may depend on the diet (i.e., the vitamin D-3 content of zoo-plankton). Contrary to general belief, no significant correlation between fat and vitamin D content was detected (72,73). The vitamin D-3 content of the frozen fish and fish products ranged between <2 (shrimp) and 196 ug/kg (roe of vendace) (72). Lu et al. (74) and Bilodeau et al. (37) found vitamin D-3 concentrations between 6 and 453 ug/kg in various fish. Where 25(OH)-D-3 was analyzed in fish and fish products, the results were consistently very low and often there was no detectable content (37,72).


Like other fat-soluble vitamins, vitamin D is absorbed incorporated in mixed micelles from the intestine into the enterocytes by nonsaturable passive diffusion. From there, vitamin D is transported along with chylomicrons via lymph to the circulation (2,75). The more polar metabolite 25(OH)D is absorbed better and faster than vitamin D because it is also taken up directly from the proximal jejunum into the portal vein (7,75).
Almost all studies on vitamin D bioavailability were done with different kinds of vitamin supplements. Vitamin D absorption from supplements varies depending on the used vehicle substance (oils, powders, and ethanol) (76). There are not a lot of data on its availability from natural sources. Based on an investigation with special pig meat (pigs receiving only vitamin D-2 as the sole vitamin D source), van den Berg (75) estimated the average relative bioavailability of vitamin D-2 from meat sources to be ~ 60% compared with a vitamin D-2 supplement.

Table 4. Published natural vitamin D-3 and 25(OH)-D-3 content in fish1

It has also been shown that the bioavailability of vitamin D from fortified hard cheese (cheddar and low-fat cheese) is equivalent to supplements (77) and that vitamin D bioavailability is not influenced by the fat content of the fortified milk (78). Apparently vitamin D-3 strongly binds to /3-lactoglobulin A as well as /3-casein, protecting it from a polar environment in fat-free dairy products. This stabilization of vitamin D-3 may ensure the availability for absorption within the human body (79). These strong binding affinities may not have a negative impact on the bioavailability because vitamin D-2 was more efficiently absorbed from fortified cheese than from water (80). Vitamin D-3 encapsulated in reformed casein micelles was highly bioavailable in a clinical study, comparable to a commercial aqueous supplement (81). Other investigations report no influence of the vehicle on the bioavailability of vitamin D supplements but a higher response of vitamin D-3 compared with vitamin D-2 (16). An influence of calcium, phosphorus, vitamin A, and cholesterol on vitamin D bioavailability is assumed, but further investigation is needed to determine the mechanism (82). Additionally, growing evidence suggests an interaction of a genetic polymorphism in key genes with bioavailability, transport, distribution in body pools, metabolism, and action of vitamin D (82,83).

Influence of processing and cooking

Cooking does not much influence the vitamin D content of animal foods. Mattila et al. (84) found that, in eggs boiled for 10 min, the vitamin D-3 concentration was 1-6% lower
and 25(OH)-D-3 content was 6-11% lower compared with raw eggs. Also in fish, the cooking effect was moderate: baking various kinds of fish (e.g., perch, rainbow trout, Baltic herring) in the oven at 172 C or 200 C for 20 min induced a vitamin D-3 loss of <10%, calculated on a dry matter basis. Only 1 lot of Baltic herring showed an exceptionally large decrease of 23%, which was attributed to the loss of fat during baking (84). Another investigation reported a loss of ~50% when salmon was fried in vegetable oil but not when it was baked (74). Cooking pork loin in the oven at 250 C for 20 min followed by 150 C until meat core temperature reached 80 C significantly increased vitamin D-3 and 25(OH)-D-3 content in all parts, presumably due to water loss and hence increased dry matter content (35). However, calculating the true retention for vitamin D-3 and 25(OH)-D-3 in the whole cut shows losses of 23% and 5%, respectively. Bennink and Ono (85) reported that for beef, 35-42% of vitamin D was lost with cooking. Purchas et al. (34) investigated the impact of several cooking procedures on vitamin D-3 and 25(OH)-D-3 content in various beef and lamb cuts. Vitamin D-3 retention in beef ranged from 79% to 101% and in lamb from 75% to 126%; retention of 25(OH)-D-3 in beef was 77%-130% and in lamb 55%-79%. In beef cuts, longer, slower cooking induced higher losses than rapid cooking. Again, water loss caused higher vitamin D-3 and 25(OH)-D-3 levels in cooked beef samples, and also in lamb, the concentrations tended to increase with cooking (34). Montgomery et al. (31,32) as well found increased vitamin D-3 and 25(OH)-D-3 concentrations in cooked (71 C internal temperature) longissimus steak, which was attributed to moisture losses.
Storing eggs at room temperature for 2 and 3 wk only leads to slight vitamin D-3 and 25(OH)-D-3 decreases (<6% on a dry matter basis) (84). Renken and Warthesen (86) investigated vitamin D stability in fortified skim milk and found some losses through exposure to light but not to air. Thermal stress like pasteurization, ultra heat treatment, sterilization, or even spray drying does not provoke a significant loss of added vitamin D in milk (87,88).


Studies focusing on the determination of the natural vitamin D content in different animal foods are limited. Most of the data derive from the development of new methods of analysis or from experiments studying the influence of feed supplementation. Although we tried to concentrate on products from animals without excessive vitamin D supplementation, often the information about the upbringing of the animals is not available (especially in retail products). This makes it difficult to compare and judge the given results, and it may also be an explanation for the variations found. Besides this, varying fat content of the products as well as other season of production may also result in different concentrations of vitamin D. The highest values of vitamin D are found in fish and especially in fish liver, ranging from <2 ug/kg to 477 ug/kg and up to 1200 ug/kg, respectively, depending on fish species and locations. Also offal provides considerable amounts of vitamin D up to 140 ug/kg, whereas the content in muscle meat is generally much lower (up to 10 ug/kg). Variations between species and meat cuts are seen. With vitamin D concentrations of up to 57 ug/kg egg yolk features values between the vitamin D values of meat and offal. Milk and dairy products are normally low in vitamin D if they are not fortified with it. The highest natural values are reported in butter and cheese (up to 10 ug/kg) due to its high fat content.
Processing does not influence the concentration of vitamin D in meat and dairy products very much because the vitamin is rather heat and oxygen tolerant. However, exposure to light can significantly reduce vitamin D content.
Because recommendations for vitamin D intake have recently been increased considerably, the possibility to cover the requirements with foodstuff is even more difficult. Nutrition societies often recommend an intake of 3 portions of dairy and 1 portion of meat, fish, or eggs per day. We estimate that by complying with these recommendations, the maximal intake of vitamin D through animal food would be 3 ug (dairy plus meat), 7 ug (dairy plus eggs), and 49 ug (dairy plus fish) per day.
Further research is needed to improve and optimize the natural vitamin D content in dairy products and meat because fortification of food is not well accepted in several countries.

Literature Cited

  • 1. Holick MF. Vitamin D Deficiency. N Engl J Med. 2007;357:266-81.
  • 2. Ball GFM. Vitamin D. Bioavailability and analysis of vitamins in foods. London: Chapman & Hall; 1998. p. 163-193.
  • 3. Working Group of the Australian and New Zealand Bone and Mineral Society; Endocrine Society of Australia; Osteoporosis Australia. Vitamin D and adult bone health in Australia and New Zealand: a position statement. Med J Aust. 2005;182:281-5.
  • 4. Holick MF. Sunlight and vitamin D: both good for cardiovascular health. J Gen Intern Med. 2002;17:733-5.
  • 5. Quarles LD. Role of FGF23 in vitamin D and phosphate metabolism: Implications in chronic kidney disease. Exp Cell Res. 2012;318:1040-8.
  • 6. Mason RS, Sequeira VB, Gordon-Thomson C. Vitamin D: the light side of sunshine. Eur J Clin Nutr. 2011;65:986-93.
  • 7. Ovesen L, Brot C, Jakobsen J. Food contents and biological activity of 25-hydroxyvitamin D: A vitamin D metabolite to be reckoned with? Ann Nutr Metab. 2003;47:107-13.
  • 8. Bischoff-Ferrari H. Health effects of vitamin D. Dermatol Ther. 2010; 23:23-30.
  • 9. Holick MF. Vitamin D status: Measurement, interpretation, and clinical application. Ann Epidemiol. 2009;19:73-8.
  • 10. Ovesen L, Andersen R, Jakobsen J. Geographical differences in vitamin D status, with particular reference to European countries. Proc Nutr Soc. 2003;62:813-21.
  • 11. IOM (Institute of Medicine). Dietary Reference Intakes for Calcium and Vitamin D. The National Academies Press; 2011.
  • 12. Deutsche Gesellschaft fur Ernahrung, Osterreichische Gesellschaft fur Ernahrung, Schweizerische Gesellschaft fur Ernahrungsforschung, Schweizerische Vereinigung fur Ernahrung. Referenzwerte fur die Nahrstoffzufuhr. 1. Auflage, 3. vollstandig durchgesehener und korrigierter Nachdruck ed. Frankfurt am Main: Umschau / Braus; 2008. German.
  • 13. Kommission der Europaischen Gemeinschaften. Richtlinie 2008/100/EG der Kommission vom 28. Oktober 2008 zur Anderung der Richtlinie 90/496/EWG des Rates uber die Nahrwertkennzeichnung von Lebensmitteln hinsichtlich der empfohlenen Tagesdosen, der Um-rechungsfaktoren fur den Energiewert und der Definitionen. (October 28, 2008). German.
  • 14. EFSA Panel on Dietetic Products NaAN. Scientific opinion on the tolerable upper intake level of vitamin D. EFSA J. 2012;10:2813-57.
  • 15. Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, Murad MH, Weaver CM. Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab. 2011;96:1911-30.
  • 16. Jakobsen J. Bioavailability and bioactivity of vitamin D3 active compounds - Which potency should be used for 25-hydroxyvitamin D3? Int Congr Ser. 2007;1297:133-42.
  • 17. Cashman KD, Seamans KM, Lucey AJ, Stocklin E, Weber P, Kiely M, Hill TR. Relative effectiveness of oral 25-hydroxyvitamin D3 and vitamin D3 in raising wintertime serum 25-hydroxyvitamin D in older adults. Am J Clin Nutr. 2012;95:1350-6.
  • 18. Tripkovic L, Lambert H, Hart K, Smith CP, Bucca G, Penson S, Chope G, Hypponen E, Berry J, Vieth R, et al. Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis. Am J Clin Nutr. 2012; 95:1357-64.
  • 19. FOPH (Federal Office of Public Health). Swiss food composition database. 2012 [cited 2012 Dec 12].
       Available from: http://www.bag.admm.ch/themen/ernaehrung_bewegung/05191/index.html?lang=en
  • 20. MRI (Max Rubner-Institut). Bundeslebensmittelschlussel. 2010 [cited 2012 Dec 12]. Available from: www.bls.nvs2.de
  • 21. ANSES -the French agency for food, environmental and occupational safety. French food composition table, Table Ciqual 2012. 2012 [cited 2012 Dec 12]. Available from: http://www.afssa.fr/TableCIQUAL/
  • 22. DTU (Technical University of Denmark - National Food Institute). Danish food composition database.2012 [cited 2012 Dec 12]. Available from: http://www.foodcomp.dk/v7/fcdb_default.asp
  • 23. United States Department of Agriculture. USDA national nutrient database for standard reference. 2011 [cited 2013 Mar 25]. Available from: http://ndb.nal.usda.gov/
  • 24. Health Canada. The Canadian nutrient file (CNF). 2012 [cited 2013 Mar 25]. Available from: http://webprod3.hc-sc.gc.ca/cnf-fce/index-eng.jsp
  • 25. Koshy KT, VanDerSlik AL. High-performance liquid chromatographic method for the determination of 25-hydroxycholecalciferol in the bovine liver, kidney, and muscle. J Agric Food Chem. 1977;25:1246-9.
  • 26. Mattila PH, Piironen VI, UusiRauva EJ, Koivistoinen PE. Contents of cholecalciferol, ergocalciferol, and their 25- hydroxylated metabolites in milk products and raw meat and liver as determined by HPLC. J Agric Food Chem. 1995;43:2394-9.
  • 27. Kobayashi T, Takeuchi A, Okano T. Vitamin D contents in various kinds of Japanese foods. In: Burckhardt P, Heaney RP, editors. Nutritional Aspects of Osteoporosis '94. Rome: Ares-Serono Symposia Publications; 1995. p. 345-349.
  • 28. Wertz AE, Knight TJ, Trenkle A, Sonon R, Horst RL, Huff-Lonergan EJ, Beitz DC. Feeding 25-hydroxyvitamin D3 to improve beef tenderness. J Anim Sci. 2004;82:1410-8.
  • 29. Montgomery JL, Carr MA, Kerth CR, Hilton GG, Price BP, Galyean ML, Horst RL, Miller MF. Effect of vitamin D3 supplementation level on the postmortem tenderization of beef from steers. J Anim Sci. 2002; 80:971-81.
  • 30. Montgomery JL, Parrish FC, Beitz DC, Horst RL, Huff-Lonergan EJ, Trenkle AH. The use of vitamin D3 to improve beef tenderness. J Anim Sci. 2000;78:2615-21.
  • 31. Montgomery JL, Blanton JR, Horst RL, Galyean ML, Morrow KJ, Wester DB, Miller MF. Effects of biological type of beef steers on vitamin D, calcium, and phosphorus status. J Anim Sci. 2004;82:2043-9.
  • 32. Montgomery JL, King MB, Gentry JG, Barham AR, Barham BL, Hilton GG, Blanton JR, Horst RL, Galyean ML, Morrow KJ, et al. Supplemental vitamin D3 concentration and biological type of steers. II. Tenderness, quality, and residues of beef. J Anim Sci. 2004;82:2092-104.
  • 33. Foote MR, Horst RL, Huff-Lonergan EJ, Trenkle AH, Parrish FC, Beitz DC. The use of vitamin D3 and its metabolites to improve beef tenderness. J Anim Sci. 2004;82:242-9.
  • 34. Purchas R, Zou M, Pearce P, Jackson F. Concentrations of vitamin D3 and 25-hydroxyvitamin D3 in raw and cooked New Zealand beef and lamb. J Food Compost Anal. 2007;20:90-8.
  • 35. Clausen I, Jakobsen J, Leth T, Ovesen L. Vitamin D-3 and 25-hydroxyvitamin D-3 in rawand cooked pork cuts. J Food Compost Anal. 2003;16:575-85.
  • 36. Wilborn BS, Kerth CR, Owsley WF, Jones WR, Frobish LT. Improving pork quality by feeding supranutritional concentrations of vitamin D3. J Anim Sci. 2004;82:218-24.
  • 37. Bilodeau L, Dufresne G, Deeks J, Clqment G, Bertrand J, Turcotte S, Robichaud A, Beraldin F, Fouquet A. Determination of vitamin D3 and 25-hydroxyvitamin D3 in foodstuffs by HPLC UV-DAD and LC-MS/MS. J Food Compost Anal. 2011;24:441-8.
  • 38. Mattila PH, Vakonen E, Valaja J. Effect of different vitamin D supplementations in poultry feed on vitamin D content of eggs and chicken meat. J Agric Food Chem. 2011;59:8298-303.
  • 39. Schmid A, Ampuero S, Butikofer U, Scherrer D, Badertscher R, Hadorn R. Nutrient composition of Swiss cooked sausages. Fleischwirtschaft Int. 2009;24:61-4.
  • 40. Schmid A, Collomb M, Scherrer D, Dubois S, Portmann R, Badertscher R, Kneubuhler H. Nutrient composition of Swiss cured meat. Fleisch-wirtschaft. 2011;91:84-8.
  • 41. Schmid A, Badertscher R, Scherrer D, Portmann R, Dubois S, Spahni M, Stoffers H. Nutrient composition of Swiss cured cooked meat. Fleischwirtschaft. 2011;91:97-100.
  • 42. Schmid A, Badertscher R, Collomb M, Dubois S, Guggisberg D, Scherrer D, Hadorn R. Compostition of various Swiss raw sausages. Mitteilungen Lebensmittel und Umweltchemie. 2013.
  • 43. Chick H, Roscoe MH. Influence of diet and sunlight upon the amount of vitamin A and vitamin D in the milk afforded by a cow. Biochem J. 1926;20:632 -49.
  • 44. Weckel KG. Vitamin D in milk a review. J Dairy Sci. 1941;24:445-62.
  • 45. Mouillet L, Luquet FM, Gagnepain MF, Sorgue Y. Dosage de la vitamine D dans le lait, par chromatographic liquide haute pression. Lait. 1982; 62:44-54.
  • 46. Kunz C, Niesen M, Lilienfeld-Toal H, Burmeister W. Vitamin D, 25-hydroxy-vitamin D and 1,25-dihydroxy-vitamin D in cow's milk, infant formulas and breast milk during different stages of lactations. Int J Vitam Nutr Res. 1984;54: 141-8.
  • 47. Takeuchi A, Okano T, Kobayashi T. Determination of vitamin-D and its metabolites in breast and cows milk. J Pharmacobiodyn. 1986;9:S71.
  • 48. Kneifel W. Analytik und Gehalt fettloslicher Vitamine in Milch und Milchprodukten - eine Uebersicht. Osterreichische Milchwirtschaft.1987;42:37-54.
  • 49. Trenerry VC, Plozza T, Caridi D, Murphy S. The determination of vitamin D3 in bovine milk by liquid chromatography mass spectrometry. Food Chem. 2011;125:1314-9.
  • 50. Jakobsen J, Saxholt E. Vitamin D metabolites in bovine milk and butter. J Food Compost Anal. 2009;22:472-8.
  • 51. Calvo MS, Whiting SJ, Barton CN. Vitamin D fortification in the United States and Canada: current status and data needs. Am J Clin Nutr. 2004;80:1710S-6S.
  • 52. Laleye LC, Wasesa AAH, Rao MV. A study on vitamin D and vitamin A in milk and edible oils available in the United Arab Emirates. Int J Food Sci Nutr. 2009;60:1-9.
  • 53. Bar A, Sachs M, Perlman R. Use of 1alpha-hydroxyvitamin D3 to prevent bovine parturient paresis. VI. Concentrations of vitamin D metabolites and vitamin D3 equivalence in milk. J Dairy Sci. 1986;69:2810-4.
  • 54. Koshy KT, VanDerSlik AL. 25-Hydroxycholecalciferol in cow milk as determined by high-performance liquid chromatography. J Agric Food Chem. 1979;27:650-2.
  • 55. Light RF, Wilson LT, Frey CN. Vitamin D in the blood and milk of cows fed irradiated yeast. J Nutr. 1934;8:105-11.
  • 56. Olson WG, Jorgense NA, Bringe AN, Schultz LH, Deluca HF. 25-Hydroxycholecalciferol (25-OH-D3). 3. Effect of dosage on soft-tissue o integrity and vitamin-D activity of tissue and milk from dairy-cows. J Dairy Sci. 1974;57:677-82.
  • 57. Hollis BW, Roos BA, Draperi HH, Lambert PW. Vitamin D and its metabolites in human and bovine milk. J Nutr. 1981;111:1240-8.
  • 58. McDermott CM, Beitz DC, Littledike ET, Horst RL. Effects of dietary vitamin D3 on concentrations of vitamin D and its metabolites in blood plasma and milk of dairy cows. J Dairy Sci. 1985;68:1959-67.
  • 59. Arrigo Y, Chaubert C, Daccord R, Gagnaux D, Gerber H, Guidon D, Jans F, Kessler J, Lehmann E et al. Futterungsempfehlungen und Nahrwerttabellen fur Wiederkauer: das grune Buch. Eidgenossische Forschungsanstalt fur Nutztiere ed. Zollikofen: Landwirtschaftliche Lehrmittelzentrale; 1999. German.
  • 60. Le Boulch N, Gulat-Marna C, Raoul Y. Derives de la vitamine D3 des laits de femme et de vache: ester sulfate de cholecalciferol et hydroxy-25 cholecalciferol. Int J Vitam Nutr Res. 1974;44:167-79.
  • 61. Okano T, Kuroda E, Nakao H, Kodama S, Matsuo T, Nakamichi Y, Nakajima K, Hirao N, Kobayashi T. Lack of evidence for existence of vitamin D and 25-hydroxyvitamin D sulfates in human breast and cow's milk. J Nutr Sci Vitaminol (Tokyo). 1986;32:449-62.
  • 62. Reeve LE, Jorgensen NA, Deluca HF, Vitamin D. Compounds in cows' milk. J Nutr. 1982;112:667-72.
  • 63. Takeuchi A, Okano T, Teraoka S, Murakami Y, Kobayashi T. High-performance liquid chromatographic determination of vitamin D in foods, feeds and pharmaceuticals by successsive use of reversed-phase and straight-phase columns. J Nutr Sci Vitaminol (Tokyo). 1984;30:11-25.
  • 64. Kurmann A, Indyk H. The endogenous vitamin D content of bovine milk - influence of season. Food Chem. 1994;50:75-81.
  • 65. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P. Determination of 25-hydroxycholecalciferol content in egg yolk by HPLC. J Food Compost Anal. 1993;6:250-5.
  • 66. Mattila P, Piironen V, Backman C, Asunmaa A, Uusi-Rauva E, Koivistoinen P. Determination of vitamin D3 in egg yolk by high-performance liquid chromatography with diode array detection. J Food Compost Anal. 1992; 5:281-90.
  • 67. Mattila P, Lehikoinen K, Kiiskinen T, Piironen V. Cholecalciferol and 25-hydroxycholecalciferol content of chicken egg yolk as affected by the cholecalciferol content of feed. J Agric Food Chem. 1999;47: 4089-92.
  • 68. Jackson PA, Shelton CJ, Frier PJ. High-performance liquid chromatographic determination of vitamin D3 in foods with particular reference to eggs. Analyst. 1982;107:1363-9.
  • 69. Sivell LM, Wenlock RW, Jackson PA. Determination of vitamin D and retinoid activity in eggs by HPLC. Hum Nutr Appl Nutr. 1982;36:430-7.
  • 70. Koshy KT, VanDerSlik AL. High-performance liquid chromatographic method for the determination of 25-hydroxycholecalciferol in chicken egg yolks. J Agric Food Chem. 1979;27:180-3.
  • 71. Yao L, Wang T, Persia M, Horst RL, Higgins M. Effects of vitamin D3-enriched diet on egg yolk vitamin D3 content and yolk quality. J Food Sci. 2013;78:178-83.
  • 72. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P. Cholecalciferol and 25-hydroxycholecalciferol contents in fish and fish products. J Food Compost Anal. 1995;8:232-43.
  • 73. Mattila P, Piironen V, Haapala R, Hirvi T, Uusi-Rauva E. Possible factors responsible for the high variation in the cholecalciferol contents of fish. J Agric Food Chem. 1997;45:3891-6.
  • 74. Lu Z, Chen TC, Zhang A, Persons KS, Kohn N, Berkowitz R, Martinello S, Holick MF. An evaluation of the vitamin D3 content in fish: Is the vitamin D content adequate to satisfy the dietary requirement for vitamin D? J Steroid Biochem Mol Biol. 2007;103:642-4.
  • 75. van den Berg H. Bioavailability of vitamin D. Eur J Clin Nutr. 1997;51: Suppl 1:S76-9.
  • 76. Grossman RE, Tangpricha V. Evaluation of vehicle substances on vitamin D bioavailability: A systematic review. Mol Nutr Food Res. 2010; 54:1055-61.
  • 77. Wagner D, Sidhom G, Whiting SJ, Rousseau D, Vieth R. The bioavailability of vitamin D from fortified cheeses and supplements is equivalent in adults. J Nutr. 2008;138:1365-71.
  • 78. Tangpricha V, Koutkia P, Rieke SM, Chen TC, Perez AA, Holick MF. Fortification of orange juice with vitamin D: a novel approach for enhancing vitamin D nutritional health. Am J Clin Nutr. 2003;77:1478-83.
  • 79. Forrest SA, Yada RY, Rousseau D. Interactions of vitamin D3 with bovine beta-lactoglobulin A and beta-casein. J Agric Food Chem. 2005;53: 8003-9.
  • 80. Johnson JL, Mistry VV, Vukovich MD, Hogie-Lorenzen T, Hollis BW, Specker BL. Bioavailability of vitamin D from fortified process cheese and effects on vitamin D status in the elderly. J Dairy Sci. 2005;88: 2295-301.
  • 81. Haham M, Ish-Shalom S, Nodelman M, Duek I, Segal E, Kustanovich M, Livney YD. Stability and bioavailability of vitamin D nanoencapsulated in casein micelles. Food Funct. 2012;3:737-44.
  • 82. Brannon PM. Key questions in vitamin D research. Scand J Clin Lab Invest Suppl. 2012;243:154-62.
  • 83. Larcombe L, Mookherjee N, Slater J, Slivinski C, Singer M, Whaley C, Denechezhe L, Matyas S, Turner-Brannen E, Nickerson P, et al. Vitamin D in a northern Canadian first nation population: dietary intake, serum concentrations and functional gene polymorphisms. PLoS ONE. 2012; 7:e49872-81.
  • 84. Mattila P, Ronkainen R, Lehikoinen K, Piironen V. Effect of household cooking on the vitamin D content in fish, eggs, and wild mushrooms. J Food Compostt Anal. 1999;12:153-60.
  • 85. Bennink MR, Ono K. Vitamin B12, E and D content of raw and cooked beef. J Food Sci. 1982;47:1786-92.
  • 86. Renken SA, Warthesen JJ. Vitamin D stability in milk. J Food Sci. 1993; 58:552-6.
  • 87. Davidek J, Velisek J, Pokorny J. Chemical changes during food processing. In: Davidek J, Velisek J, Pokorny J, editors. Developments in food science. 21 ed. Amsterdam,The Netherlands: Elsevier Science Publishers; 1990. p. 284.
  • 88. Indyk H, Littlejohn V, Woollard DC. Stability of vitamin D-3 during spray-drying of milk. Food Chem. 1996;57:283-6.
  • 89. Egaas E, Lambertsen G. Naturally occurring vitamin D3 in fish products analysed by HPLC, using vitamin D2 as an international standard. Int J Vitam Nutr Res. 1979;49:35-42.


Attached files

ID Name Comment Uploaded Size Downloads
2812 Vitamin D in Animal products.pdf admin 16 Jul, 2013 644.95 Kb 984