All cells of the body need Vitamin D
For about 30% of the people the Vitamin D Receptor limits the D in the blood from getting to the cells
- Uterine Fibroids (uterine leiomyoma, need hysterectomy) 1.9 more likely if a Vitamin D receptor problem – Sept 2015
- Uterine fibroids (hysterectomy) 32 percent more likely if less than 20 ng vitamin D – May 2013
- Endometriosis treated, and perhaps prevented, by vitamin D
Which is an editorial for the following study
Vitamin D as an effective treatment in human uterine leiomyomas independent of mediator complex subunit 12 mutation
Fertil Steril. 2020 Oct 7;S0015-0282(20)30710-X. doi: 10.1016/j.fertnstert.2020.07.049
Ana Corachán 1, María Gabriela Trejo 1, María Cristina Carbajo-García 2, Javier Monleón 3, Julia Escrig 3, Amparo Faus 1, Antonio Pellicer 4, Irene Cervelló 1, Hortensia Ferrero 5
Objective: To study whether vitamin D (VitD) inhibits cell proliferation and Wnt/β-catenin and transforming growth factor-β (TGFβ) signaling pathways in uterine leiomyomas independent of mediator complex subunit 12 (MED12) mutation status.
Design: Prospective study comparing leiomyoma vs. myometrial tissues and human uterine leiomyoma primary (HULP) cells treated with or without VitD and analyzed by MED12 mutation status.
Setting: Hospital and university laboratories.
Patient(s): Women with uterine leiomyoma without any treatment (n = 37).
Intervention(s): Uterine leiomyoma and myometrium samples were collected from women undergoing surgery because of symptomatic leiomyoma pathology.
Main outcome measure(s): Analysis of Wnt/β-catenin and TGFβ pathways and proliferation by quantitative real-time polymerase chain reaction in leiomyoma and myometrial tissue as well as in VitD-treated HULP cells analyzed by Sanger sequencing.
Results: Sequencing data showed that 46% of leiomyomas presented MED12 mutation, whereas no mutations were detected in adjacent myometrium. Expression of Wnt/β-catenin and TGFβ pathway genes was significantly increased in MED12-mutated leiomyomas compared to matched myometrium; no significant differences were found in wild-type (WT) leiomyomas. In HULP cells, VitD significantly decreased PCNA expression of both MED12-mutated and WT groups. VitD treatment decreased WNT4 and β-catenin expression in both groups compared to controls, with significance for WNT4 expression in MED12-mutated samples. Similarly, VitD significantly inhibited TGFβ3 expression in cells from both groups. MMP9 expression also decreased.
Conclusion: Despite molecular differences between MED12-mutated and WT leiomyomas, VitD inhibited Wnt/β-catenin and TGFβ pathways in HULP cells, suggesting VitD as an effective treatment to reduce proliferation and extracellular matrix formation in different molecular subtypes of uterine leiomyomas.