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Oxidative stress to DNA prevented by Vitamin D (H2O2, rats) – Jan 2019

Vitamin D Treatment Reverses the Induced Oxidative Stress Damage to DNA.

Pak J Biol Sci. 2019 Jan;22(1):8-14. doi: 10.3923/pjbs.2019.8.14
Haq SH, AlAfaleq NO, Johari RA.


BACKGROUND AND OBJECTIVE: The aim of the current study was to investigate in detail the effect of the active metabolite of vitamin D3 [1, 25 (OH)2 D3] in ameliorating the induced oxidative damage to DNA.

MATERIALS AND METHODS:
Primary cortical neuron cultures from one week old Wister rats were set up in sterile conditions. The neuron cultures were maintained for up to 72 h in culture in the presence of varying doses of vitamin D. Cells were exposed to (0.5 mM H2O2) for 2 h prior to collection of condition medium and cell pellet for Biochemical Assays. Control and H2O2 treated cultures were maintained without any treatment with vitamin D.

RESULTS: Pre-treatment with 0.25 μg mL-1 for 24 and 48 h significantly reduced the oxidative stress. 8-hydroxydeoxyguanosine a ubiquitous marker of oxidative stress had also shown to be significantly reduced. The DNA damage marker PolyUB of histones was observed in the neuron treated with H2O2 only.

CONCLUSION:
This study revealed that oxidation of DNA by hydrogen peroxide caused extensive DNA damage, resulting in polyubiquitination of histones. The pre-treatment with vitamin D3 however completely reversed the DNA damage cascade induced by hydrogen peroxide and protected the DNA.

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