Saudi Med J. 2014 Oct;35(10):1243-9.
Sadat-Ali M1, Al-Elq AH, Al-Shaikh IH, Al-Turki HA, Al-Ali AK, Al-Othman AA.
1Department of Orthopedic Surgery, College of Medicine, King Fahd Hospital of the University, University of Dammam, Dammam, Kingdom of Saudi Arabia. E-mail. drsadat at hotmail.com.
|Mean||22 ng||17 ng||14 ng|
|% > 30 ng||22 %||9 %||6%|
The varation of results between test systems continues to be a huge problem
- Problems with Vitamin D Testing – chapter – Aug 2019
- Technical comparison of vitamin D testers – July 2013
- Vitamin D testing continues to have lots of varition between systems – Nov 2012
- Vitamin D test results still vary by about 9 percent – March 2012
- Vitamin D test results vary as much as 30 ng between test labs – Jan 2012
- Canadians – 37 percent have less than 20 ng of vitamin D (after adjusting test results with standards) – Sept 2015
- Big differences (~10 ng) in vitamin D test results, even when using the same type of tester – Aug 2015
- Vitamin D measurements vary with the same sample of blood – March 2014
- All items in category Test for D
- Low cost vitamin D Blood Tests
- Different measurements from 6 vitamin D testing systems – Mar 2012 which has the following
To compare the performance of 3 commonly used 25-hydroxyvitamin D (25-OHD) assays among a sample of the Saudi population.
This cross-sectional study was carried out between January 2011 and December 2012 at King Fahd Hospital of the University, Al-Khobar, Saudi Arabia. After informed consent, blood samples for measurement of 25-OHD level was extracted from 200 adults. The vitamin D level of each individual were determined using chemiluminescence immunoassay (CLIA), radio-immuno assay (RIA), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay. Assays were also compared through commonly used cut-points for classification of vitamin D deficiency. Bias between assays was evaluated using Bland-Altman plots.
The average age of patients was 45.7+/-16.1 years. A significant difference between the assays was found. The mean 25-OHD levels were highest for the LC-MS/MS (21.65 ng/mL, 95% CI 19.74-23.56), intermediate for RIA (16.607 ng/mL, 95% CI 14.87-18.32), and lowest for CLIA method (13.864 ng/mL, 95% CI 12.109-15.618). Using 30 ng/mL as a cutoff value, only 6% was found to have normal levels of 25-OHD using CLIA, 9% using RIA, and 22% using LC-MS/MS.
Levels of 25-OHD and the prevalence of vitamin D deficiency are dependent on the assay used. The reported high prevalence of hypovitaminosis D among the Saudi population can be partially explained by the use of assays that underestimate vitamin D levels.
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