Clin Chim Acta. 2012 Mar 20.
Ong L, Saw S, Sahabdeen NB, Tey KT, Ho CS, Sethi SK.
Department of Laboratory Medicine, National University Hospital, Singapore.
Vitamin D testing is becoming increasingly important with recent research demonstrating a correlation between vitamin D insufficiency and metabolic diseases, immunodeficiencies and other diseases. However, existing 25-hydroxyvitamin D (25OHD) assays lack comparability to the candidate reference method, causing difficulties in diagnosis and monitoring of vitamin D deficiency.
We looked at the accuracy of 3 automated assays (Roche Diagnostics Elecsys® Total 25OHD assay, Abbott Architect® Total vitamin D assay, Advia Centaur® vitamin D Total assay) and Diasorin® Radioimmunoassay (RIA) compared to a routine laboratory Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
The correlation based on Passing Bablok regression was good with the slopes between 0.95 and 1.31 and the intercepts between -3.24 and 3.68. However, a significant positive bias was observed using the Abbott Architect and the Diasorin RIA.
Using published analytical goals of coefficient of variation (CV) <10% and bias <5%, most methods did not meet these criteria.
Using measurement of uncertainty of 9%, most methods were able to meet criteria using quality control materials but not patient samples.
Inadequacies of these assay performances are contributed by differences in method of extraction of vitamin D from vitamin D binding protein, cross-reactivities to 25OHD(2), 25OHD(3) and other vitamin D metabolites, matrix interferences and a lack of standardization.
Copyright © 2012. Published by Elsevier B.V.