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Technical comparison of vitamin D testers – July 2013

Determination of vitamin D and its metabolites

Best Practice & Research Clinical Endocrinology & Metabolism
Christopher-John Farrell, FRCPA, FAACBa, 1 (Chemical Pathologist),
Markus Herrmann, FRCPAb, markusherr at aol.com (A/Prof. Dr. med. habil)
a Laverty Pathology, 60 Waterloo Rd, North Ryde, NSW 2113, Australia
b District Hospital of Bolzano, Department of Clinical Pathology, Böhler Strasse 5, 39100 Bozen, Italy

http://dx.doi.org/10.1016/j.beem.2013.06.001, How to Cite or Link Using DOI

The demand for analysis of 25-hydroxyvitamin D has increased dramatically throughout the world over the past decade. As a consequence, a number of new automated assays have been introduced for 25-hydroxyvitamin D measurement. Automated assays have shown variable ability to meet the technical challenges associated with 25-hydroxyvitamin D measurement. Assays are able to meet performance goals for precision at high concentrations but fail to do so at low concentrations of 25-hydroxyvitamin D. The overall accuracy of automated methods has improved over recent years and generally shows good overall agreement with reference methods; however, discrepancies persist for individual samples. Liquid chromatography-tandem mass spectrometry is used by some routine laboratories for 25-hydroxyvitamin D analysis but its widespread use is hampered by limited sample throughput. 1,25-Dihydroxyvitamin D is an important analyte in specific clinical situations, which remains in the hands of specialised laboratories using manual analytical methods.

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Technical comparison of vitamin D testers – July 2013

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- Problems with Vitamin D Testing – chapter – Aug 2019 Vitamin D measurements vary with the same sample of blood – March 2014 Huge variation in Vitamin D test results between 4 testers for 8 people – 2013 Vitamin D levels observed to vary by 20 percent during a day – Feb 2019 Percent who are Vitamin D Deficient: 6, 9, or 22 – depends on testing system – Oct 2014 Previous VitaminDWiki News

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