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Technical comparison of vitamin D testers – July 2013

Determination of vitamin D and its metabolites

Best Practice & Research Clinical Endocrinology & Metabolism
Christopher-John Farrell, FRCPA, FAACBa, 1 (Chemical Pathologist),
Markus Herrmann, FRCPAb, markusherr at aol.com (A/Prof. Dr. med. habil)
a Laverty Pathology, 60 Waterloo Rd, North Ryde, NSW 2113, Australia
b District Hospital of Bolzano, Department of Clinical Pathology, Böhler Strasse 5, 39100 Bozen, Italy

http://dx.doi.org/10.1016/j.beem.2013.06.001, How to Cite or Link Using DOI

The demand for analysis of 25-hydroxyvitamin D has increased dramatically throughout the world over the past decade. As a consequence, a number of new automated assays have been introduced for 25-hydroxyvitamin D measurement. Automated assays have shown variable ability to meet the technical challenges associated with 25-hydroxyvitamin D measurement. Assays are able to meet performance goals for precision at high concentrations but fail to do so at low concentrations of 25-hydroxyvitamin D. The overall accuracy of automated methods has improved over recent years and generally shows good overall agreement with reference methods; however, discrepancies persist for individual samples. Liquid chromatography-tandem mass spectrometry is used by some routine laboratories for 25-hydroxyvitamin D analysis but its widespread use is hampered by limited sample throughput. 1,25-Dihydroxyvitamin D is an important analyte in specific clinical situations, which remains in the hands of specialised laboratories using manual analytical methods.

PDF is attached at the bottom of this page

See also VitaminDWiki

Attached files

ID Name Comment Uploaded Size Downloads
2828 Vitamin D assays July 2013.jpg admin 22 Jul, 2013 11:37 77.70 Kb 1173
2827 Determination of vitamin D and its metabolites.pdf PDF 2013 admin 22 Jul, 2013 11:34 388.38 Kb 9166
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