Nutrients 2013, 5, 3605-3616; doi:10.3390/nu5093605
Fahad Alshahrani and Naji Aljohani 2,3,4,*
1 Department of Medicine, King Abdulaziz Medical City, Riyadh 14611, Saudi Arabia; E-Mail: fahad_alshahrani at yahoo.com
2 Specialized Diabetes and Endocrine Center, King Fahad Medical City, Riyadh 59046, Saudi Arabia; E-Mail: najijohani at gmail.com
3 Faculty of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh 22490, Saudi Arabia
4 Prince Mutaib Chair for Biomarkers of Osteoporosis, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
* Author to whom correspondence should be addressed; E-Mail: najijohani at gmail.com; Tel.: +966-1-467-5939; Fax: +966-1-467-5931.
Received: 6 May 2013; in revised form: 21 August 2013 /Accepted: 27 August 2013 / Published: 13 September 2013
Table of contents
- 1 Introduction
- 2. Vitamin D Metabolism
- 3. Optimum 25OHD Levels
- 4. Measurements of 25OHD versus 1,25OH2D3
- 5. Supplementation of Vitamin D2 versus Vitamin D3
- 6. Candidates for Calcidiol (25-OHD) Measurements
- 7. Vitamin D Correction
- 8. Vitamin D Toxicity
- 9. Hypersensitivity to Vitamin D
- 10. Conclusions
- See also VitaminDWiki
Abstract: The plethora of vitamin D studies over the recent years highlight the pleomorphic effects of vitamin D outside its conventional role in calcium and bone homeostasis. Vitamin D deficiency, though common and known, still faces several challenges among the medical community in terms of proper diagnosis and correction. In this review, the different levels of vitamin D and its clinical implications are highlighted. Recommendations and consensuses for the appropriate dose and duration for each vitamin D status are also emphasized.
Vitamin D plays an essential role in the regulation of metabolism, calcium and phosphorus absorption of bone health. However, the effects of vitamin D are not limited to mineral homeostasis and skeletal health maintenance. The presence of vitamin D receptors (VDR) in other tissue and organs suggest that vitamin D physiology extends well above and beyond bone homeostasis . Additionally, the enzyme responsible for the conversion of 25[OH] D to its biologically active form [Vitamin D (1,25[[OH]2 D)] has been identified in other tissues aside from kidneys [2,3], and that extra renal synthesis of 1,23[OH]2D may be equally important in regulating cell growth and differentiation via paracrine or autocrine regulatory mechanisms .
The mechanism of action of vitamin D3 through its hormonal form, dihydroxyvitaminD3, involves a nuclear VDR that regulates the transcription of several target genes in a variety of vitamin D target cells that are primarily involved in the calcium homeostasis of cell differentiation . Hypervitaminosis D occurs when pharmacologic doses of vitamin D are consumed for prolonged periods of time or from a single megadose translating to a large increase in circulating 25[OH]D concentrations .
Vitamin D has two distinct forms: vitamins D2 and D3. Vitamin D2 is a 28-carbon molecule derived from ergosterol (a component of fungal cell membranes), while vitamin D3 is a 27-carbon derived from cholesterol . UV-B irradiation of skin triggers photolysis of 7-dehydrocholesterol (pro-vitamin D3) to pre-vitamin D3, which is rapidly converted to vitamin D3 by the skin's temperature. Vitamin D (D2 and D3) from the skin and diet undergo two sequential hydroxylations: first in the liver (25[OH]D) and then in the kidney, leading to its biologically active form 1,25-dihydroxyvitamin D (1,25[OH]2D) . Table 1 shows the nomenclature for vitamin D precursors and metabolites.
Table 1. Nomenclature of vitamin D precursors and metabolites.
The 1,25 [OH]2D ligand binds with high affinity to vitamin D receptors (VDRs), which then increases intestinal absorption of both calcium and phosphorus. In addition, vitamin D is actively involved in bone formation, resorption, mineralization, and in maintenance of neuromuscular function. Circulating 1,25[OH]2D inhibits serum parathyroid hormone (PTH) levels by negative feedback mechanism and by increased serum calcium levels. It also regulates bone metabolism through activation of the VDRs found in osteoblasts, releasing biochemical signals and leading to the formation of mature osteoclasts .
In a low vitamin D state, the small intestine can absorb approximately 10%-15% of dietary calcium. When adequate however, intestinal absorption of dietary calcium rises to approximately 30%-40% [9,10]. Hence, low vitamin D levels (25[OH]D) may lead to insufficient calcium absorption, and this has clinical implications not only for bone health but also for most metabolic functions. The increase in PTH restores calcium homeostasis by increasing tubular reabsorption of calcium in the kidney, increasing bone calcium mobilization and enhancing 1,25[OH]2D production .
The vitamin D level needed to optimize intestinal calcium absorption (34 ng/mL) is lower than the level needed for neuromuscular performance (38 ng/mL) [11,12]. Experts however believe that the lower limit of adequate 25[OH]D levels should be 30 ng/mL . Still others recommend a lower limit of 40 ng/mL, since impaired calcium metabolism due to low serum 25[OH]D levels may trigger secondary hyperparathyroidism, increased bone turnover and progressive bone loss [14,15].
The proposed 25[OH]D cut-off for optimum skeletal health is the level that reduces PTH to a minimum and increases calcium absorption to its maximum [11,16]. Several studies have shown that PTH levels plateau at a minimum steady-state level as serum 25[OH]D levels approach and rise above approximately 30 ng/mL (75 nmol/L) [16-18]. The established consensus of several vitamin D cut-offs is presented in Table 2 [18-20]. It is noteworthy, however, that there is a continued debate and exchange of knowledge with respect to the optimum cut-off for 25(OH)D.
Table 2. Diagnostic Cut-Offs of levels of serum 25[OH]D.
The clinical advantages of choosing 25[OH]D instead of calcitriol as a marker for vitamin D status has been listed by Rajasree et al. . First, 25[OH]D has the highest concentration of all vitamin D metabolites. Second, its levels remain stable for almost two weeks. Lastly, vitamin D toxicity is thought to be a function of 25[OH]D instead of calcitriol. It has been observed that serum 25[OH]D is the best indicator of vitamin D status among individuals without kidney disease . Furthermore, 25[OH]D in large amounts can replace calcitriol to stimulate bone calcium metabolism . Although nephrectomy abolishes a response to physiological dose of 25[OH]D, a large dose (1000 fold) of 25[OH]D can stimulate intestinal calcium absorption and bone calcium metabolism in nephrectomized rats . Hughes et al., studied vitamin D intoxication in two human patients with normal kidney function and showed that both patients had 16-fold above normal concentrations of plasma 25[OH]D levels (500-600 ng/mL), while 1,25[OH]D2D3 plasma concentrations were only modestly elevated (40-56 pg/mL) . Differences in calcidiol versus calcitriol are presented in Table 3.
Table 3. Calcidiol versus Calcitriol.
Multiple preparations of vitamin D and its metabolites are commercially available for supplement use. The two most common supplements are ergocalciferol (vitamin D2) and cholecalciferol (vitamin D3). Some studies [26,27], but not all , suggest that vitamin D3 increases serum 25[OH]D more efficiently than vitamin D2. A large, single dose of vitamin D2 does not last longer than a large dose of D3. In a study conducted by Armas et al., , subjects were given one dose of 50,000 IU of either vitamin D2 or vitamin D3. Vitamin D2 was absorbed just as well as vitamin D3, yet blood levels of 25[OH]D started dropping rapidly after 3 days among subjects given vitamin D2 whereas those on vitamin D3 sustained high levels for two weeks before dropping gradually.
A daily dose of 4000 IU of vitamin D3 for two weeks was observed to be 1.7 times more effective in raising 25[OH]D levels than 4000 IU of vitamin D2 . On the other hand, Holick et al. found that a daily dose of 1000 IU of vitamin D2 over 11 weeks duration increased 25[OH]D levels from 42 to 67 nmoL/L (16.9 to 26.8 ng/mL) . Consequently, vitamin D3 levels also increased from 49 to 72 nmoL/L (19.6 to 28.9 ng/mL). It took 6 weeks for 25[OH]D levels to plateau on that regimen. In another study, Glendenning et al. compared 1000 IU of D2 versus D3 in patients who had vitamin D insufficiency with subsequent hip fractures. After three months, those who were supplemented with D3 had a 31%-52% greater increase in 25[OH]D levels than those supplemented with D2. However, parathyroid hormone levels did not differ between groups .
In children, Gordon et al., assigned 40 infants and toddlers with vitamin D deficiency to one of three regimens (2000 IU oral vitamin D2 daily, 50,000 IU vitamin D2 weekly or 2000 IU vitamin D3 daily) for 6 weeks. At the end of the trial, 25[OH]D levels increased from 42.5 to 90 nmoL/L and there were no significant differences between treatment groups .
In terms of bioavailability, Biancuzzo et al., tested changes in 25[OH]D status from a daily dose of 1000 IU of vitamin D2 or D3 from either calcium-fortified orange juice with vitamin D or supplement capsules for 11 weeks. The average 25[OH]D levels of all groups (D2 from orange juice, D2 from capsules, D3 from orange juice, D3 from capsules) went up to about 25 nmoL/L with no significant differences between groups .
Treatment for most studies found D2 to be less effective than D3, whereas in studies finding them equally effective, the treatment was daily amounts between 400 and 2000 IU . Houghton and Vieth indicated that vitamin D3 is the most potent form of vitamin D in all primate species, including humans, owing to the diminished binding of vitamin D2 metabolites to DBP in plasma . They also confirmed the finding of Hollick , which indicated that the difference in binding capacity is potentially explained by the presence of a methyl group at carbon-24 position on the D2 molecule. The different hydroxylation sites of two forms of vitamin D leads to the production of unique biologically active metabolites. Based on this, the 24-hydroxylation after the 25-hydroxylation results in the formation of 1,24,25[OH]3D2 and the deactivation of vitamin D2 molecule. On the other hand, the vitamin D3 metabolite 1,24,25[OH]3D3 must undergo an additional side chain oxidation to be biologically deactivated . Interestingly, 1,24,25[OH]3D3 has the ability to bind VDR with ~40% capacity higher than with 1,25[OH]2D3 .
The best indicator of vitamin D status is 25-OHD because it reflects cutaneous and dietary intake, not to mention it is the major circulating form of vitamin D . While there are many established causes of vitamin D deficiency, as listed in Table 4, screening for the general population warrants further investigation. The United States Preventive Services Task Force (USPSTF) did not comment for or against routine screening for vitamin D deficiency. One approach is to consider serum testing in patients at high risk for vitamin D deficiency, and treating without testing those at a lower risk . Just recently, a statement from Osteoporosis Canada suggested that based on clinical suspicion for vitamin D insufficiency and its complications the clinical approach can take into account three settings
Table 4. Major causes of vitamin D deficiency .
Reduced skin synthesis
Sunscreen, skin pigment, season/time of day, aging Cystic fibrosis, celiac disease, Crohn's disease, gastric bypass, medications that reduce cholesterol absorption Obesity (BMI > 30) Anti-convulsant, glucocorticoid Exclusively without vitamin D supplementation Hepatic failure Nephrotic proteinuria Chronic renal failure Vitamin D resistance
Increased sequestration Increased catabolism Breastfeeding Decreased synthesis of 25-hydroxyvitamin D Increased urinary loss of 25-hydroxyvitmain D Decreased synthesis of 1,25-dihydroxyvitmain D Inherited disorders
__Table 5._- Approach to vitamin D correction .
In patients with normal absorptive capacity, for every 40 IU/day (1 ug/day) of vitamin D3, serum 25(OH)D concentrations increase by approximately 0.3 to 0.4 ng/mL (0.7 to 1.0 nmol/L) . Largest increments are seen in patients with the lowest starting 25(OH)D level, but subsequently declines as 25(OH)D concentration reaches 40 ng/mL (100 nmol/L) . Nutritional deficiency (25OHD < 50 nmol/L) requires initial treatment with 50,000 units of vitamin D2 or vitamin D3 orally once per week for 6-8 weeks, and then 800 to 1000 IU of vitamin D3 orally thereafter . Intramuscular cholecalciferol (300,000 IU) in one or two doses per year is also an option for increasing serum 25 OHD level .
Nutritional insufficiency (25 OHD 50-75 nmol/L) requires treatment with 800 to 1000 IU of vitamin D3 daily. This intake will bring the average adult's vitamin D status to 7 nmol/L higher over a three-month period. Still, many individuals might need higher doses. In malabsorptive states, oral dosing and duration of treatment is dependent on the individual patient's on vitamin D absorptive capacity. High doses of vitamin D (10,000 to 50,000 IU daily) may be necessary for patients who had gastrectomy or malabsorption history. Patients who remain deficient or insufficient on such doses need to be treated with hydroxylated vitamin D metabolites, since they are more readily absorbed than with ordinary sun or sun camp exposure. All patients should maintain a daily calcium intake of at least 1000 mg (for ages 31 to 50 years) to 1200 mg (for ages 51 and older) per day .
Vitamin D as a fat-soluble vitamin raised concerns about toxicity from excessive supplementation. Widespread vitamin D fortification of foods and drinks from the 1930s to 1950s in the United States and Europe led to reported cases of toxicity . Hypercalcemia is responsible for producing most of the symptoms of vitamin D toxicity. Early symptoms of vitamin D toxicity include gastrointestinal disorders like anorexia, diarrhea, constipation, nausea, and vomiting. Bone pain, drowsiness, continuous headaches, irregular heartbeat, loss of appetite, muscle and joint pain are other symptoms that are likely to appear within a few days or weeks; frequent urination, especially at night, excessive thirst, weakness, nervousness and itching; kidney stones . There are three major hypotheses for vitamin D toxicity :
- (i) Raised plasma 1,25[OH]D concentrations lead to increased intracellular 1,24[OH]D concentrations. This hypothesis is not widely supported as many studies revealed that vitamin D toxicity is associated with normal or marginally elevated 1,25[OH]D . It was only Mawer et al. who reported elevated 1,25[OH]D with vitamin D toxicity .
- (ii) Vitamin D intake raises plasma 25[OH]D levels to concentrations that exceed DBP binding capacity, and free 25[OH]D has direct effects on gene expression once it enters target cells. High dietary vitamin D intake alone increases plasma 25[OH]D. The low affinity of 1,25[OH]D for the transport protein DBP and its high affinity for VDR dominate normal physiology. This makes it the only ligand with access to the transcriptional signal transduction machinery. However, in vitamin D intoxication, overloading by various vitamin D metabolites significantly compromises the capacity of the DBP by allowing other metabolites to enter the cell nucleus. Of all the inactive metabolites, 25[OH]D has the strongest affinity for the VDR, and thus at sufficiently high concentrations, could stimulate transcription .
- (iii) Vitamin D intake raises the concentrations of many vitamin D metabolites, including vitamin D itself and 25[OH]D, and these concentrations exceed the DBP binding capacity and release of "free" 1,25[OH]D which enters target cells .
The amount of UVB radiation required for vitamin D sufficiency can be calculated from the amount of vitamin D produced from one minimal erythemal dose (MED), or 10,000-25,000 IU of oral vitamin D .The MED can be defined as the amount of time needed to cause skin to turn pink. The length of time varies with geographical location, skin pigmentation, percent of body fat, and age. Excessive exposure to sunlight will not cause vitamin D intoxication because sunlight degrades any excess vitamin D .
The highest recorded individual serum 25[OH]D concentration obtained from sunshine was from a farmer in Puerto Rico with a level of 225 nmol/L . On the other hand, the highest recorded individual 25[OH]D achieved from artificial ultraviolet light treatment sessions was 275 nmol/L . Vieth reported that vitamin D toxicity probably begins to occur after chronic consumption of approximately 40,000 IU/day (100 of the 400 IU capsules) . Reports in which pharmacologic doses of vitamin D were given for a prolonged time, the indications why it was given and in which the final serum 25[OH]D concentrations are provided and summarized in Table 6.
Vitamin D hypersensitivity syndromes are often mistaken for vitamin D toxicity. The most common is primary hyperparathyroidism. Granulomatous diseases, such as sarcoidosis, granulomatous TB and some cancers also cause vitamin D hypersensitivity, as the granuloma or the tumor may make excessive amounts of calcitriol, thus raising serum calcium levels .
Table 6. Studies reporting elevated vitamin D status and associated diseases.
The present review discussed current knowledge on vitamin D physiology, its clinical relevance and evidence-based treatment options on vitamin D status correction. Caution should still be practiced by clinicians in providing vitamin D supplementation among vitamin D deficient populations, with proper monitoring using approved and certified methods. Indications for vitamin D supplementation outside the conventional calcium homeostasis should also be considered to maximize extra-skeletal benefits of vitamin D correction.
Conflicts of Interest
The authors declare no conflict of interest.
- 1. DeLuca, H. Overview of General physiological tenures and function of vitamin D. Am. J. Clin. Nutr. 2004, 80, 16895-16965.
- 2. Mawer, E.B.; Hayes, M.E.; Heys, S.E.; Davies, M.; White, A.; Stewart, M.F.; Smith, G.N. Constitutive synthesis of 1,25 dihydroxy vitamin D3 by a human small cell lung cancer cell line. J. Clin. Endocrinol. Metab. 1994, 79, 554-560.
- 3. Schwartz, G.G.; Whitlutch, L.W.; Chen, T.C.; Lokeshwar, B.L.; Holick, M.F. Human prostate cells synthesize 1,25 dihydroxyvitamin D3. Cancer Epidemiol. Biomark. Prev. 1998, 7, 391-395.
- 4. Holick, M.F. Sunlight, vitamin D and health: A D-lightful story. Nor. Acad. Sci. Lett. 2008, 2008, 147-166.
- 5. Jones, G.; Strugnell, S.; DeLuca, H.F. Current understanding of the molecular action of vitamin D. Physiol. Rev. 1998, 78, 1193-1231.
- 6. Vieth, R. Vitamin D supplementation, 25 hydroxy-vitamin D concentrations, and safety. Am. J. Clin. Nutr. 1999, 69, 842-856.
- 7. Ahmed, M.S.; Shoker, A. Vitamin D metabolites; protective versus toxic properties: Molecular and cellular perspectives. Nephrol. Rev. 2010, 2, 19-26.
- 8. Holick, M.F. High prevalence of vitamin D inadequacy and implications for health. Mayo Clin. Proc. 2006, 87, 353-373.
- 9. Holick, M.F. Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers and cardiovascular disease. Am. J. Clin. Nutr. 2004, 80, 1678S-1688S.
- 10. Holick, M.F. Vitamin D: the underappreciated D-lightful hormone that is important for skeletal and cellular health. Curr. Opin. Endocrinol. Diabetes 2002, 9, 87-98.
- 11. Heaney, R.P.; Dowell, M.S.; Hale, C.A.; Bendich, A. Calcium absorption varies within the reference range for serum 25-hydroxyvitamin D. J. Am. Coll. Nutr. 2003, 22, 142-146.
- 12. Bischoff-Ferrari, H.A.; Dietrich, T.; Orav, E.J.; Hu, F.B.; Zhang, Y.; Karlson, E.W.; Dawson-Hughes, E.B. Higher 25 hydroxy-vitamin D concentration are associated with better lower-extremity function in both active and inactive persons aged > 60 yrs. Am. J. Clin. Nutr. 2004, 80, 752-758.
- 13. Holick, M.F. Vitamin D deficiency. N. Engl. J. Med. 2007, 357, 266-281.
- 14. Cannell, J.J.; Hollis, B.W.; Zasloff, M.; Heaney, R.P. Diagnosis and treatment of vitamin D deficiency. Expert Opin. Pharmacother. 2008, 9, 107-118.
- 15. Lips, P. Vitamin D deficiency of secondary hyperparathyroidism in the elderly: Consequences for bone loss and fractures and therapeutic implications. Endocr. Rev. 2001, 22, 477-501.
- 16. Chapuy, M.C.; Preziosi, P.; Maamer, M.; Arnaud, S.; Galan, P.; Hercberg, S.; Meunier, P.J.; Prevalence of vitamin D insufficiency in an adult normal population. Osteopros. Int. 1997, 7, 439-443.
- 17. Holick, M.F.; Siris, E.S.; Binkley, N.; Beard, M.K.; Khan, A.; Katzer, J.T.; Petruschke, R.A.; Chen, E.; de Papp, A.E. Prevalence of vitamin D inadequacy among postmenopausal North American women receiving osteoporosis therapy. J. Clin. Endocrinol. Metab. 2005, 90, 3215-3224.
- 18. Heaney, P.R. Functional indices of vitamin D status and ramifications of vitamin D deficiency. Am. J. Clin. Nutr. 2004, 80, 1706S-1709S.
- 19. Hanley, D.A.; Davison, K.S. Vitamin D insufficiency in North America. J. Nutr. 2005, 735, 332-337.
- 20. Hollis, B.W.; Wagner, C.L. Assessment of dietary vitamin D requirements during pregnancy and lactation. Am. J. Clin. Nutr. 2004, 79, 717-726.
- 21. Rajasree, S.; Rajpal, K.; Kartha, C.C.; Sarma, P.S.; Kutty, V.R.; Iyer, C.S.; Girija, G. Serum 25-dihyroxyvitamin D3 levels are elevated in South Indian patients with ischemic heart disease. Eur. J. Epidemiol. 2001, 77, 567-571.
- 22. Wang, T.J.; Pencina, M.J.; Booth, S.L.; Jacques, P.F.; Ingelsson, E.; Lanier, K.; Benjamin, E.J.; DAgostino, R.B.; Wolf, M.; Vasan, R.S. Vitamin D deficiency and risk of cardiovascular disease. Circulation 2008, 777, 503-511.
- 23. Shepard, M.R.; Deluca, H.F. Plasma concentrations of vitamin D3 and its metabolites in the rat as infused by vitamin D3 intake. Arch. Biochem. Biophys. 1980, 202, 43-53.
- 24. Paulovitch, H.; Gurabedian, M.; Bulsan, S. Calcium mobilizing effect of large doses of 25-dyroxycalciferol ion anephric rats. J. Clin. Investig. 1973, 52, 2656-2659.
- 25. Hughes, M.R.; Baylink, D.J.; Jones, P.J.; Haussler, M.R. Radioligand receptor assay for 25-hydroxvitamin D2/D3 and 1 alpha,25-hydroxyvitamin. J. Clin. Investig. 1976, 58, 61-70.
- 26. Trang, H.M.; Cole, D.E.; Rubin, L.A.; Pierratos, A.; Siu, S.; Vieth, R. Evidence that vitamin D3 increases serum 25-hydroxyvitamin D more efficiently than does vitamin D2. Am. J. Clin. Nutr. 1998, 68, 854-858.
- 27. Armas, L.A.; Hollis, B.W.; Heaney, R.P. Vitamin D2 is much less effective than vitamin D3 in humans. J. Clin. Endocrinol. Metab. 2004, 89, 5387-5391.
- 28. Holick, M.F.; Biancuzzo, R.M.; Chen, T.C.; Klein, E.K.; Young, A.; Bibuld, D.; Reitz, R.; Salameh, W.; Ameri, A.; Tannenbaum, A.D. Vitamin D2 is as effective as vitamin D3 in maintaining circulating concentrations of 25-hydroxyvitamin D. J. Clin. Endocrinol. Metab. 2008, 93, 677-681.
- 29. Glendenning, P.; Chew, G.T.; Seymour, M.J.; Goldswain, P.R.; Inderjeeth, C.A.; Vasikaran, S.D.; Toronto, M.; Musk, A.A.; Fraser, W.D. Serum 25 hydroxyvitamin D levels in vitamin D insufficient hip fracture patients after supplementation wit ergocalciferol and cholecalciferol. Bone 2009, 45, 870-875.
- 30. Gordon, C.M.; Williams, A.L.; Feldman, H.A.; May, J.; Sinclair, L.; Vasquez, A.; Coz, J.E. Treatment of hypovitaminosis D in infants and toddlers. J. Clin. Endocrinol. Metab. 2008, 93, 2716-2721.
- 31. Biancuzzo, R.M.; Young, A.; Bibuld, D.; Cai, M.H.; Winter, M.R.; Klein, E.K.; Ameri, A.; Reitz, R.; Salameh, W.; Chen, T.C.; et al. Fortification of orange juice with vitamin D2 or vitamin D3 is as effective as an oral supplement in maintaining vitamin D status in an adult. Am. J. Clin. Nutr. 2010, 97, 162-166.
- 32. Chan, J. Vitamin D update for nutrition professionals. Veget. Nutr. 2009, 78, 1-2.
- 33. Houghton, A.L.; Vieth, R. The case against ergocalciferol (vitamin D2 as a vitamin supplement). Am. J. Clin. Nutr. 2006, 84, 694-697.
- 34. Hollis, B.W. Comparison of equilibrium and disequilibrium assay conditions of ergocalciferol and cholicalciferol and their metabolites. J. SteroidBiochem. 1989, 27, 81-86.
- 35. Horst, R.L.; Reinhardt, T.A.; Ramberg, C.F.; Koszewski, N.J.; Napoli, J.L. 24-Hydroxylation of 1,25-dihydroxyergocalciferol: An unambiguous deactivation process. J. Biol. Chem. 1986, 267, 9250-9256.
- 36. Jones, G. Analog Metabolism in Vitamin D; Feldman, D., Glorieux, F., Pike, J.W., Eds.; Elsevier Academic Press: Melville, NY, USA, 1997; pp. 73-94.
- 37. Institute of Medicine Standing Committee on the Scientific Evaluation of Dietary Reference Intakes. Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride; National Academy Press: Washington, DC, USA, 1997.
- 38. Teresa, K.; Amy, G.; Jackie, R.; Jennie, H.; Sarina, S. Vitamin D: An evidence based review. J. Am. Board. Fam. Med. 2009, 22, 698-706.
- 39. Hanley, D.A.; Cranney, A.; Jones, G.; Whiting, S.J.; Leslie, W.D. Guidelines Committee of the Scientific Advisory Council of Osteoporosis of Canada. Vitamin D in adult health and disease; a review and guideline statement from Osteoporosis Canada (summary). Can. Med. Assoc. J. 2010, 782, 1315-1319.
- 40. Dawson-Hughes, B. Treatment of Vitamin D Deficient States, 2010. Wolters Kluwer Health Website. Available online: http://www.update.com/contents/treatment-of-vitamin-d-deficiency-in-adults (accessed on 11 September 2013).
- 41. Vieth, R. Critique of the consideration for establishing the tolerable upper intake level for vitamin D: Critical need for revision upwards. J. Nutr. 2006, 736, 1117-1122.
- 42. Dawson-Hughes, B.; Heaney, R.P.; Holick, M.F.; Lips, P.; Meunier, P.J.; Vieth, R. Estimates of optimal vitamin D status. Osteoporos. Int. 2005, 76, 713-716.
- 43. De Torrente de la Jara, G.; Pecoud, A.; Favrat, B. Female asylum seekers with musculoskeletal pain; the importance of diagnosis and treatment of hypovitaminosis D. BMC Fam. Pract. 2006, 7, 4.
- 44. Institute of Medicine (US) Standing Committee on the Scientific Evaluation of Dietary Reference Intakes. Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride; National Academies Press: Washington, DC, USA, 1997.
- 45. Holick, M.F. Vitamin D deficiency: What a pain it is. Mayo Clin. Proc. 2003, 78, 1457-1459.
- 46. Schwalfenberg, G. Not enough vitamin D: health consequences for Canadians. Can. Fam. Phys. 2007, 53, 841-854.
- 47. Jones, G. Pharmacokinetics of vitamin D toxicity. Am. J. Clin. Nutr. 2008, 88, 5825-5865.
- 48. Mawer, E.B.; Hann, J.T.; Berr, J.L.; Davies, M. Vitamin D metabolism in patients intoxicated with ergocalciferol. Clin. Sci. (Lond.) 1985, 68, 135-141.
- 49. Haddock, L.; Corcino, J.; Vazquez, M.D. 25 OHD serum level in the normal Puerto Rican population and in subject with tropical sprue and parathyroid disease. Puerto Rico Health Sci. J. 1982, 7, 85-91.
- 50. Krause, R.; Burning, M.; Hopfenmuller, W.; Holick, M.F.; Sharma, A.M. Ultraviolet B and blood pressure. Lancet 1998, 352, 709-710.
- 51. Mason, R.S.; Lissner, D.; Grunstein, H.S.; Posen, S. A simplified assay for dihydroxylated vitamin D metabolites in human serum: Application to hyper- and hypovitaminosis D. Clin. Chem. 1980, 26, 444-450.
- 52. Gertner, J.M.; Domenech, M. 25-Hydroxyvitamin D levels in patients treated with high-dosage ergo- and cholecalciferol. Clin. Pathol. 1977, 30, 144-150.
- 53. Counts, S.J.; Baylink, D.J.; Shen, F.H.; Sherrard, D.J.; Hickman, R.O. Vitamin D intoxication in an anephric child. Ann. Intern. Med. 1975, 82, 196-200.
- 54. Streck, W.F.; Waterhouse, C.; Haddad, J.G. Glucocorticoid effects in vitamin D intoxication.Arch. Intern. Med. 1979, 739, 974-977.
- 55. Davies, M.; Adams, P.H. The continuing risk of vitamin-D intoxication. Lancet 1978, 2, 621-623.
- 56. Allen, S.H.; Shah, J.H. Calcinosis and metastatic calcification due to vitamin D intoxication. A case report and review. Horm. Res. 1992, 37, 68-77.
- 57. Rizzoli, R.; Stoermann, C.; Ammann, P.; Bonjour, J.P. Hypercalcemia and hyperosteolysis in vitamin D intoxication: Effects of clodronate therapy. Bone 1994, 75, 193-198.
- 58. Pettifor, J.M.; Bikle, D.D.; Cavaleros, M.; Zachen, D.; Kamdar, M.C.; Ross, F.P. Serum levels of free 1,25-dihydroxyvitamin D in vitamin D toxicity. Ann. Intern. Med. 1995, 722, 511-513.
- 59. Jacobus, C.H.; Holick, M.F.; Shao, Q.; Chen, T.C.; Holm, I.A.; Kolodny, J.M.; Fuleihan, G.E.; Seely, E.W. Hypervitaminosis D associated with drinking milk. N. Engl. J. Med. 1992, 326, 1173-1177.
PDF is attached at the bottom of this page