25-Hydroxyvitamin D3 suppresses hepatitis C virus production – Oct 2012

 

25-Hydroxyvitamin D3 suppresses hepatitis C virus production

Hepatology. 2012 Oct;56(4):1231-9. doi: 10.1002/hep.25763.
Matsumura T, Kato T, Sugiyama N, Tasaka-Fujita M, Murayama A, Masaki T, Wakita T, Imawari M.
Division of Gastroenterology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan.

Because the current interferon (IFN)-based treatment for hepatitis C virus (HCV) infection has a therapeutic limitation and side effects, a more efficient therapeutic strategy is desired. Recent studies show that supplementation of vitamin D significantly improves sustained viral response via IFN-based therapy. However, mechanisms and an active molecular form of vitamin D for its anti-HCV effects have not been fully clarified. To address these questions, we infected HuH-7 cells with cell culture-generated HCV in the presence or absence of vitamin D(3) or its metabolites.

To our surprise, 25-hydroxyvitamin D(3) [25(OH)D(3) ], but not vitamin D(3) or 1,25-dihydroxyvitamin D(3) , reduced the extra- and intracellular levels of HCV core antigen in a concentration-dependent manner. Single-cycle virus production assay with a CD81-negative cell line reveals that the inhibitory effect of 25(OH)D(3) is at the level of infectious virus assembly but not entry or replication. Long-term 25(OH)D(3) treatment generates a HCV mutant with acquired resistance to 25(OH)D(3) , and this mutation resulting in a N1279Y substitution in the nonstructural region 3 helicase domain is responsible for the resistance. Conclusion: 25(OH)D(3) is a novel anti-HCV agent that targets an infectious viral particle assembly step. This finding provides insight into the improved efficacy of anti-HCV treatment via the combination of vitamin D(3) and IFN.

Our results also suggest that 25(OH)D(3) , not vitamin D(3) , is a better therapeutic option in patients with hepatic dysfunction and reduced enzymatic activity for generation of 25(OH)D(3) .

Copyright © 2012 American Association for the Study of Liver Diseases.

PMID: 22487892

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25(OH)D(3) =Calcidiol is better than Vitamin D for reducing HCV
This should not be a surprise. The virus reduces the liver's ability to convert Vitamin D into 25(OH)D(3)

 Download the PDF from VitaminDWiki.

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Association between vitamin D and hepatitis C virus infection: a meta-analysis. - Sept 2013

World J Gastroenterol. 2013 Sep 21;19(35):5917-24. doi: 10.3748/wjg.v19.i35.5917.
Villar LM1, Del Campo JA, Ranchal I, Lampe E, Romero-Gomez M.

AIM:
To evaluate the association between 25-hydroxyvitamin D [25(OH)D] and sustained virological response (SVR) in hepatitis C virus (HCV) infected individuals.
METHODS:
Relevant studies were identified by systematically searching MEDLINE databases up to March 2012 and abstracts of the European and American Congress of Hepatology conducted in 2011. Studies must provide information on SVR and the levels of 25(OH)D₃ and/or 25(OH)D₂ [henceforth referred to as 25(OH)D] in sera samples from HCV infected individuals. The inclusion criteria were: clinical studies that included HCV infected patients aged older than 18 years regardless of HCV genotype or ethnic group; provided information on SVR rates; and were reported in the English language as full papers. Due to the heterogeneity of studies in categorizing serum vitamin D levels, a cut-off value of 30 ng/mL of serum 25(OH)D was used. Heterogeneity was assessed using I² statistics. The summary odds ratios with their corresponding 95%CI were calculated based on a random-effects model.
RESULTS:
Overall, 11 studies (8 observational and 3 interventional) involving 1575 individuals were included and 1117 HCV infected individuals (71%) showed low vitamin D levels. Most of the studies included mono-infected HCV individuals with the mean age ranging from 38 to 56 years. Four studies were conducted in human immunodeficiency virus/HCV infected individuals. Regarding vitamin D measurement, most of the studies employed radioimmunoassays (n = 5) followed by chemiluminescence (n = 4) and just one study employed high performance/pressure liquid chromatography (HPLC). Basal vitamin D levels varied from 17 to 43 ng/mL in the studies selected, and most of the HCV infected individuals had genotype 1 (1068/1575) with mean viral load varying from log 4.5-5.9 UI/mL. With regard to HCV treatment, most of the studies (n = 8) included HCV individuals without previous treatment, where the pooled SVR rate was 46.4%. High rates of SVR were observed in HCV individuals with vitamin D levels above 30 ng/mL (OR = 1.57; 95%CI: 1.12-2.2) and those supplemented with vitamin D (OR = 4.59; 95%CI: 1.67-12.63) regardless of genotype.
CONCLUSION:
Our results demonstrated high prevalence of vitamin D deficiency and high SVR in individuals with higher serum vitamin D levels or receiving vitamin D supplementation.

PMID: 24124339
 Download the PDF from VitaminDWiki.

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See also VitaminDWiki

• Overview Liver and vitamin D
• All Liver items and Vitamin D 105 items
• Vitamin D3 becomes Calcidiol which becomes Calcitriol
• Calcidiol may be 5X more effective than Vitamin D3 – June 2012
• Overview Liver and vitamin D probably should consider Calcidiol if have liver problems or perhaps have had gallbladder removed
• Nano-encapsulated of Vitamin D3, Calcidiol, calcitriol look promising, esp time release – Dec 2012 7 day!!
• Search VitaminDWiki for "Hepatitis C" OR HCV 69 items as of April 2014
• Vitamin D can inhibit enveloped virus (e.g. Corona, Herpes, Bird Flu, Epstein, Hepatitis, RSV, etc.) – March 2011
• Vitamin D prevents Hepatitis-C and helps treat it (many studies)

See also Clinical Trials

• Search for "Hepatitis C" with Vitamin D intervention 16 studies as of March 2018
• Efficacy and Safety of the Combination Vitamin D With Standard of Care in Egyptian Patients With Untreated Chronic Hepatitis C (ViZIR) 28,000 IU/week
• Can Vitamin D Supplementation Improve Hepatitis C Cure Rates (ViaDUCT) 100,000 IU monthly
• Effects of Vitamin D on Inflammation in Liver Disease 500,000 IU one time

See also PubMed

• Search for "hepatitis c" (Calcidiol OR Cacitriol) 51 studies as of March 2018
• Association between vitamin D and hepatitis C virus infection: a meta-analysis Sept 2013 full free text online
  Our results demonstrated high prevalence of vitamin D deficiency and high SVR in individuals with higher serum vitamin D levels or receiving vitamin D supplementation.
• Vitamin D for your patients with chronic hepatitis C? Jan 2013 - PDF is attached at the bottom of this page
• Theoretical basis of a beneficial role for vitamin D in viral hepatitis Oct 2012, full free text online
  In conclusion, vitamin D could have a beneficial role in viral hepatitis. Calcitriol is best used for viral hepatitis. . .
  So, VitaminDWiki wonders why there are no clinical trials using Calciriol to treat Hepatitis C
• Interplay between vitamin D status and antiviral therapy among chronic hepatitis C Egyptian patients April 2015
  Vitamin D levels went up after HCV treatment
• Vitamin D status and viral response to therapy in hepatitis C infected children Jan 2015
  " Cases treated with vitamin D showed significant higher early (P<0.04) and sustained (P<0.05) virological response"
  RCT with 2,000 IU of vitamin D  Download the PDF from VitaminDWiki

See also web

• For Drugs That Save Lives, a Steep Cost New York Times, April 2014
  Sovaldi, the first drug to cure hepatitis C, costs $86,000 for a six-week course. .
• Hepatitis C: Cure, but at What Cost? Medscape May 2014
  95% cure rate but costs $151,200 ($1800/day for 12 weeks )
• Harvoni, a Hepatitis C Drug From Gilead, Wins F.D.A. Approval NYT Oct 2014
  New drug costs Only $63,000 for 8 weeks. (Calcidiol or equiv, might cost $630 for 8 weeks)

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