Vitamin D Metabolite Profiling and genes (CYP24A1, CYP27B1)– Nov 2021

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Diagnostic Aspects of Vitamin D: Clinical Utility of Vitamin D Metabolite Profiling

JMBR https://doi.org/10.1002/jbm4.10581
Glenville Jones,Martin Kaufmann

Ratio of metabolites provides an interesting view

The assay of vitamin D that began in the 1970s with the quantification of one or two metabolites, 25-OH-D or 1,25-(OH)2D, continues to evolve with the emergence of liquid chromatography tandem mass spectrometry (LC-MS/MS) as the technique of choice. This highly accurate, specific, and sensitive technique has been adopted by many fields of endocrinology for the measurement of multiple other components of the metabolome, and its advantage is that it not only makes it feasible to assay 25-OH-D or 1,25-(OH)2D but also other circulating vitamin D metabolites in the vitamin D metabolome. In the process, this broadens the spectrum of vitamin D metabolites, which the clinician can use to evaluate the many complex genetic and acquired diseases of calcium and phosphate homeostasis involving vitamin D. Several examples are provided in this review that additional metabolites (eg, 24,25-(OH)2D3, 25-OH-D3-26,23-lactone, and 1,24,25-(OH)3D3) or their ratios with the main forms offer valuable additional diagnostic information. This approach illustrates that biomarkers of disease can also include metabolites devoid of biological activity. Herein, a case is presented that the decision to switch to a LC-MS/MS technology permits the measurement of a larger number of vitamin D metabolites simultaneously and does not need to lead to a dramatic increase in cost or complexity because the technique uses a highly versatile tandem mass spectrometer with plenty of reserve analytical capacity. Physicians are encouraged to consider adding this rapidly evolving technique aimed at evaluating the wider vitamin D metabolome toward streamlining their approach to calcium– and phosphate-related disease states.
 Download the PDF from VitaminDWiki

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VitaminDWiki - CYP27B1 category listing contains the following

The CYP27B1 gene activates Vitamin D in the Kidney,    Skin,    Lungs,    Brain,   Eyes   Breasts   etc.
Poor CYP27B1 is assocated with COVID, Miscarriage,   Lupus,   Alz, Parkinson, MSA,   Rickets

CYtochrome P450 family 27 subfamily B member 1    = 25-Hydroxyvitamin D3 1-alpha-hydroxylase

• 63 items in CYP27B1 category
• 343 articles in the Genetics
• 530 articles in Vitamin D Receptor
• 178 articles in Vitamin D Binding Protein
• CYP27B1 and other genes are less activated in seniors
• CYP27B causes many health problems – March 2020
• Every Parkinson’s brain had a poor CYP27B1 gene

What can be done if have a poor CYP27B1

• Larger doses of Vitamin D
• More Bio-available: Gut-friendly form, Topical form, taken with fatty meal, taken with evening meal
• Additional sources: UV
• Increase Vitamin D metabolism: additional Magnesium, Omega-3
  • All cytochrome P450 enzymes require Mg++ as a cofactor
• Increase the amount of Vitamin D in the blood that gets to cells: increase activation of VDR
  • See: Reasons for low response to vitamin D

Vitamin D blood test misses CYP27B1 and other genes

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VitaminDWiki pages with CYP24A1 in title (27 as of Dec 2021)

This list is automatically updated