Vitamin D fortification of food - review Dec 2011

The Potential Role of Vitamin D Enhanced Foods in Improving Vitamin D Status

Nutrients 2011, 3, 1023-1041; doi:10.3390/nu3121023
OPEN ACCESS
Louise O'Mahony 1,2, Magdalena Stepien 1,2, Michael J. Gibney , Anne P. Nugent and Lorraine Brennan 1A correspondence lorraine.brennan at ucd.ie
1 UCD Institute of Food and Health, University College Dublin, Belfield, Dublin 4, Ireland, E-Mails: louise.omahony at ucd.ie (L.O.M.); magdalena.stepien at ucd.ie (M.S.); mike.gibney at ucd.ie (M.J.G.); anne.nugent at ucd.ie (A.P.N.)
2 UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
Received: 2 November 2011; in revised form: 23 November 2011 /Accepted: 6 December 2011 / Published: 16 December 2011

Abstract: Low vitamin D intake and status have been reported worldwide and many studies have suggested that this low status may be involved in the development of several chronic diseases. There are a limited number of natural dietary sources of vitamin D leading to a real need for alternatives to improve dietary intake. Enhancement of foods with vitamin D is a possible mode for ensuring increased consumption and thus improved vitamin D status. The present review examines studies investigating effects of vitamin D enhanced foods in humans and the feasibility of the approach is discussed.

---

See also VitaminDWiki

• 5000 IU of vitamin D in daily bread resulted in 50 ng and improved quality of life– May 2014
• Feed chicken lots of vitamin D, get 6000 IU per egg with no change in production – Nov 2013
• Vitamin D home fortification- don't wait 100 years for your govt
• A plea for vit D food fortification in India May 2010
• All items in category Fortification with vitamin D 135 items
• Food fortified by 440 IU increased vitamin D levels by 8 ng – meta-analysis April 2012
• Overview Fortification of Vitamin D

---

Vitamin D is responsible for a wide range of functions in the body and these known roles are expanding as research in this field accumulates. The discovery in 1969 of the nuclear vitamin D receptor (VDR) for 1,25(OH)2D stimulated a vast amount of research that to date has collectively described the presence of the VDR in at least 38 human tissues and organs [1]. The VDR was first described in the bone, kidney and gastrointestinal tract but since has been identified in a diverse range of other tissues including those in the brain, breast, colon and prostate. The VDR, a phosphoprotein, facilitates the various biological functions of calcitriol by binding to the active hormone with high affinity and regulating the expression of genes via zinc finger-mediated DNA binding and protein-protein interactions [2].

The well established functions of vitamin D relate to calcium absorption and homeostasis, with the role for vitamin D in bone mineralization and bone health extensively documented and long recognised, particularly in relation to rickets and osteomalacia [3,4]. Vitamin D, in tandem with calcium, may also be involved in the prevention of osteoporosis, fracture incidence and falls, particularly in older populations [5,6]. However, vitamin D is no longer regarded as just a calcaemic hormone as a result of the widespread distribution of the VDR and the fact that the consequences of insufficiency have been shown to extend beyond the above classic effects. Though the evidence is less robust, vitamin D deficiency is now implicated in a host of other diseases including psoriasis, multiple sclerosis, inflammatory bowel disease, type 1 and 2 diabetes, hypertension, cardiovascular disease, the metabolic syndrome and various cancers [7,8]. A thorough examination of all these functions is beyond the scope of the present review and the reader is referred to a number of good quality reviews [7,9].

In parallel with the growing evidence for widespread action for vitamin D there is robust data demonstrating that an increased vitamin D status is linked to significant benefits in terms of morbidity and mortality risk reduction. A recent review demonstrated that an estimated 20% reduction of global vitamin D-sensitive disease mortality and up to 17.6% of all-cause mortality could be achieved by doubling vitamin D status [10]. In Nordic populations improvement of vitamin D status may decrease the risk of mortality from a number of common diseases including cancer and cardiovascular disease between 19 and 24%, depending on the country [10]. For Canada, which shares a similar northerly latitude, reductions of 16.1% were estimated [11]. These findings corroborate those of a number of other studies that have demonstrated that mortality is greatest amongst individuals with the lowest vitamin D status [12,13].

Taking into account the multiple functions of vitamin D and the disease risk-reduction potential, it has become evident that maintaining an adequate status is important. Vitamin D status is assessed by measuring the circulating form of the vitamin (25(OH)D) in serum. There is much debate in the literature as to which cut-offs to use for defining vitamin D deficiency. However, recent research has lead investigators to increase the cut-off for deficiency to 50 nmol/L (from 25 nmol/L). Concomitant with this, serum concentrations of 75 nmol/L or higher have been established as adequate levels, with values in between indicating insufficiency [14-16].

Estimates of global vitamin D status indicate widespread inadequacy, with severe hypovitaminosis D most common in the Middle East and South Asia [15]. Even within Europe, large variance in serum concentrations exist between countries [17]. Though individuals living at higher latitudes commonly exhibit low 25(OH)D serum concentrations due to reduced UVB radiation, especially during winter months [18-20], high levels of inadequacy have also been found in Southern Europe, particularly among older populations [21,22]. In the UK, estimates suggest that only 18% and 24.1% of women and men, respectively, have adequate 25(OH)D concentrations [19]. Higher 25(OH)D levels have been reported in Canada, where 35% of the population have levels above 75 nmol/L [23]. In the US, 58.4% and 27% of adults have serum 25(OH)D concentrations >50 nmol/L and >75 nmol/L, respectively [24,25].

Stemming from the reports of low levels of circulating 25(OH)D, certain countries recommend an exposure of 10-15 min daily of sunshine [26,27]. However, due to geographical, cultural and behavioural differences between populations, increased UVB radiation exposure is not sufficient to maintain healthy vitamin D levels, especially during winter. Therefore, optimal dietary vitamin D intake is crucial to maintain adequate vitamin D status. The recommendation for vitamin D intake differs between countries in Europe and ranges from 0 to 15 microgram for different population groups [28]. According to a recent report of the Institute of Medicine, the RDA for vitamin D should be 15 microgram/day for children greater than 1 year old and adults, and 20 microgram/day for the elderly [29]. As such, there is a renewed interest in improving dietary intake in an effort to meet these recommendations. Additionally, if we are to counteract population level vitamin D deficiency enhancement of dietary intake is the only feasible mode.

The main objective of the present review is to provide an overview of the vitamin D containing foods available, to include natural, fortified and enriched sources. It will describe the randomized controlled trials that have been performed with vitamin D enhanced foods and the effectiveness of these in raising serum 25(OH)D levels. The current regulations governing the addition of vitamin D to foods in Europe will also be outlined.

2. Food Intakes and Sources of Vitamin D

A recent trans-European examination of nutrient intake patterns showed dietary intakes of vitamin D to differ markedly between countries. Intakes were significantly higher in Nordic countries including Sweden, Denmark and Norway than in Mediterranean regions such as Italy and France [30]. Intakes of less than 3.0 microgram/day and 2.0 microgram/day have been reported in Italian and Spanish adults [31,32]. Older men in Finland have some of the highest dietary intakes in Europe at 9.0 microgram/day [33]. Recent estimates from Ireland revealed intakes ranging from 3.9 microgram to 8.5 microgram/day in adults, with an increase observed with age [34]. In the US, mean dietary consumption for adults ranges from 3.6 to 5.6 microgram/day [35], lower than the population mean of 5.8 microgram/day in Canada [36]. Intakes in Japanese adults appear higher, at >6 microgram of vitamin D per 1000 kilocalories of energy intake per day [37].

Foods that make the highest contribution to dietary intakes of vitamin D vary from country to country according to habitual dietary patterns. In Norway, the predominant food sources are fish and fats [38], as is the case in Finland where liquid milk and dairy products also contribute significantly [33]. In the UK, oily fish (24%), followed by meat and meat products (22%) and cereal and cereal products (21%) contribute the highest percentage to average daily vitamin D intakes in adults [39]. For Irish adults, meat (30%), fish (12%) and spreads (10%) made the most significant contributions of all food groups [34]. In Spain the main source is fish which accounts for 65% of intake [31]. This can be contrasted with the US where vitamin D fortified milk makes by far the highest contribution to intakes (58% in men, 39% in women) [40], figures comparable to those of Canada where milk products account for 49% of dietary vitamin D intake [36]. In Japan, the major sources of dietary vitamin D intake are fish/shellfish (79%) and eggs (9%) [41], though fish consumption has been shown to contribute >90% of vitamin D intake in an elderly Japanese cohort [42].

People are often reliant on dietary intake of vitamin D to satisfy their requirements, however foods naturally rich in vitamin D are few in number and in many cases not widely consumed. Table 1 illustrates the levels of vitamin D generally found in a number of food sources as reported in food consumption databases and in the scientific literature. As can be seen in Table 1, the richest sources include fish liver oils, oily fish, egg yolk, and wild mushrooms. However, even in those foods considered the richest sources of vitamin D, the levels are highly variable. For example, the content of vitamin D in fish can vary significantly both between and within species and according to whether they are wild or farmed; the vitamin D content of farmed salmon has been shown to be only approximately 25% that of wild salmon [43].

Table 1. Vitamin D content of selected foods.
Food
Vitamin D Content (ug/100 g)

Fish

Cod liver oil 210.0 [44]-250.0 [45]
Salmon, wild (Pacific) 13.1 [45]-24.7 [43]
Salmon, wild (Atlantic) 5.9 [44]
Salmon, farmed * 6.0 [43]
Herring, Baltic, raw 5.7-15.4 [46]
Kipper fillets, raw 8.0 [44]
Mackerel, raw 8.8 [44]-16.1 [45]
Sardines, canned in brine 4.6 [44]
Tuna, canned in oil 6.7 [45]
Anchovy, canned in oil 1.7 [45]-3.0 [44]
Cod Trace [44]-2.6 [43]
Sole Trace [44]-2.8 [45]

Mushrooms *

Mushrooms, wild edible (Cantharellus tubaeformis) 13.6 [46]-29.8 [47]
Mushrooms, chanterelle 5.3 [45]-14.2 [48]
Mushrooms, white button (Agaricus bisporus) 0.2 [45]
Mushrooms, white button (Agaricus bisporus), UV irradiated 11.9 [49]

Dairy

Milk, whole, unfortified 0.1 [45]
Milk, fortified 1.3 [45]-2.0 [50]
Cheese, cheddar 0.3 [44]-0.6 [45]
Yoghurt, plain 0.1 [45]

Meat and Eggs

Liver, beef, raw 1.2 [45]
Beef, rib eye steak, raw 0.1 [45]
Pork, cured bacon, 1.6 [45]
Ham 0.7 [45]-1.1 [44]
Chicken, breast, raw 0.1 [45]
Turkey, slices 2.2 [44]
Butter 1.5 [45]
Egg, whole, raw 1.8 [44]-2.05 [45]
Egg, yolk, raw 4.9 [44]-5.4 [45]

Cereal products

Breakfast cereals, fortified
2.8-8.3 [44]

Bread, fortified 3.0 [51]

* Levels dependent on feed; * Vitamin D in the form of vitamin D2.
Note: Vitamin D intakes are discussed in terms of micrograms (fig) or International Units (IU).
In Europe (ig are most commonly adopted as the measure of vitamin D. In order to convert (ig to IU multiply by 40.

Fortified foods are those to which one or more essential nutrients have been added, whether or not it is normally contained in the food, for the purpose of preventing or correcting a demonstrated deficiency [52]. Enriched foods are those that have nutrients that were originally lost during processing restored and are sometimes considered interchangeably with fortified foods [53]. It is also possible to enhance animal products by feeding animals vitamin D supplemented feed, thereby increasing the levels of vitamin D present in the edible products. In the current review, unless otherwise stated, we will consider animal and non-animal fortified foods, and enriched foods under the umbrella term of vitamin D-enhanced foods.

At present, the number and variety of vitamin D fortified foods available on the market differs significantly between countries but most commonly includes milk, breakfast cereals and margarines. The relative deficit of vitamin D fortified foods can be partly attributed to the country-specific policies on food fortification which are not yet unified. Most individual countries have their own national policies, even within Europe, which tend to limit the number or type of foods that can be fortified, as will be discussed later in the review. Even in those countries where vitamin D food fortification is mandatory, current levels of fortification in permitted foods are likely inadequate to satisfy physiological requirements [54]. Furthermore, the small number of foods available is likely to have limited impact on population dietary intake. For example, certain population groups are low consumers of milk, one of the more commonly fortified foods [40]. As such, whilst it is important that currently fortified foods continue to be widely available and consumed, novel methods for producing a wider variety of vitamin D-rich foods are also increasingly sought.

In recent years, evidence has emerged demonstrating viable new methods for enhancement of foods with vitamin D. Fungi can produce considerable vitamin D2 levels when exposed to UVB light through the conversion of natural ergosterol, the rate depending on the irradiation dose and temperature [55,56]. The vitamin D content in these irradiated mushrooms appears relatively stable when refrigerated [57] and the retention of vitamin D in mushrooms following cooking and storage has been reported as high, at 86% and greater [46]. Even after 6 years of storage, dried mushrooms appear to retain much of their vitamin D content [48]. Success in terms of vitamin D enhancement of animal products has been seen with pigs, fish and hens. Increasing the dietary vitamin D3 content of pigs diets can produce meat and liver with higher levels of vitamin D3 [58,59]. Similarly, the levels of vitamin D in fish can be improved through the feeding of vitamin D3-rich feed, with >10% of the dietary content of vitamin D3 retrieved in the whole fish [60]. The vitamin D content of eggs can also be safely increased by feeding hens vitamin D3-rich diets. The consumption of one of these eggs could provide up to 2.8 (ig of vitamin D, approximately 3 times that of a typical egg, with scope for increasing this even further [61,62]. The preservation of vitamin D3 and 25-hydroxyvitamin D3 in egg yolk after cooking is high and only modestly affected by storage, with losses of <10% in both cases [46]. Little work has been done on modelling the impact that the widespread introduction of such enhanced foods might have on the vitamin D intakes of populations. To assess the potential effectiveness of such eggs in increasing vitamin D intakes, we simulated the increase that would result from the consumption of enhanced eggs by Irish adults. Based upon current estimated intakes of 112 g of eggs and egg dishes per week, the consumption of eggs with a vitamin D content of 2.8 microgram versus 1.1 microgram (per 60 g egg) would result in a weekly population intake of 5.2 microgram vitamin D from egg products versus the current estimated intake of 2.1 microgram. By excluding those people that do not consume egg products, intake increases from 4.2 microgram to 10.8 microgram per week. Further assessment of the potential impact of a range of vitamin D enhanced foods on population intakes of this vitamin is certainly warranted. To this end we have performed food modelling work which will be discussed in the section examining the regulation surrounding the sale of foods enhanced with vitamin D.

3. Foods Enhanced with Vitamin D

As outlined above, it is feasible to enhance a variety of foods with vitamin D; however, the biological significance of consumption of these foods needs to be assessed. In order to assess the efficacy of vitamin D enhanced products in increasing serum 25(OH)D concentration, a number of randomized controlled trials (RCTs) have been conducted in recent years. The present review focuses on the RCTs which provided at least 10microgram of vitamin D2 or D3 each day with foods that were enhanced with vitamin D [63-71] (Table 2). A literature search was conducted using the PubMed and Medline databases up to May 2011 for studies in the English language. Keywords used included "vitamin D" and foods that could be fortified with vitamin D, such as "cheese", "bread", "orange juice", "yogurt", "spreads" and "margarine", "cereals", and "mushrooms".

In general, the majority of the studies concentrated on markers of bone metabolism as well as vitamin D status and as a consequence most analysed serum 25(OH)D, PTH and calcium concentration; some included collection of a three day food diary. Most of the interventions were three to twelve weeks duration and were performed during winter months, when the cutaneous synthesis of vitamin D is low and does not contribute to circulating levels of 25(OH)D. Two out of nine studies had a longer duration of twenty-four months. Across the nine studies volunteers were generally healthy, representing both genders and age groups ranging from 18 to 87 years (Table 2).

Table 2. Change in serum 25(OH)D after intake of foods enhanced with vitamin D.
Food source
References
Daily dose (jig) vitamin D (jig vitamin D/100 g product)
Duration and population
Study groups (product portion)
Change from baseline of serum 25(OH)D (%)

Fortified milk

Chee et al., 2003 [64]
10
(2.2 ng/100 g)
24 months
50-65 years, postmenopausal females n = 200
1. vitamin D3 + Ca-fortified skimmed milk
2. Usual diet
25.0 # 4.1 #

Daly et al., 2006 [65]
20 Hg (5.0 ng/100 g)
24 months
50-87 years, ambulatory community-living
(male)
n = 167
1. Vitamin D3 + Ca-fortified UHT milk, reduced fat
2. Usual diet
7.4 # -19.9 #

Fortified yogurt drink

Nikooyeh et al., 2011 [68]
25 Hg (5.0 ng/100 g)
12 weeks, October-March 30-60 years, diabetic subjects (fasting blood glucose >126 mg/dL) n = 90
1. Vitamin D3-fortified yogurt drink
2. Vitamin D3 + Ca-fortified yogurt drink
3. Plain yogurt drink
75.0
67.6
-10.6

Fortified cheese

Johnson et al., 2005 [66]
(study 1)
15 ^g (17.6 ng/100 g)
2 winter months
>60 years, subjects (total serum cholesterol <240 mg/dL) n = 110
1. Vitamin D3-fortified process cheese
2. Placebo process cheese
3. No cheese
-8.7
10.0 5.6

Wagner et al., 2008 [71]
100 ng * (2083.3 or 1690.8 ng/100 g) **
8 weeks, January-April 18-60 years, healthy subjects n = 80
1. Vitamin D3-fortified regular fat cheddar cheese ***
2. Vitamin D3-fortified reduced fat cheddar cheese
3. Vitamin D3 supplement to be taken with food
4. Vitamin D3 supplement to be taken without food
5. Placebo regular fat cheddar cheese
6. Placebo supplement
128.8 120.7
106.5 111.0 -7.8 ##

Fortified orange juice

Tangpricha et al., 2003 [69]
(study 2)
25 Hg (10.4 ng/100 g)
12 weeks, commenced in March 22-60 years, healthy subjects
n = 30
1. Vitamin D3 + Ca-fortified orange juice
2. Placebo Ca-fortified orange juice
150.0 45.0

Biancuzzo et al., 2010 [63]
25 Hg (10.6 ng/100 g)
11 weeks, commenced in February 18-79 years, healthy subjects n = 105
1. Vitamin D3 orange juice A + placebo capsule
2. Vitamin D2 orange juice + placebo capsule
3. Vitamin D3 capsules + placebo orange juice
4. Vitamin D2 capsules + placebo orange juice
5. Placebo capsule + placebo orange juice
71.5
67.1
42.9
65.1
-8.6

UV enhanced mushrooms

Urbain et al., 2011 [70]
100 ng * (191.0 ng/100 g) **
3 weeks + 2 weeks follow up, January-March Healthy female and male <45 years n = 27
1. Vitamin D2 soup + placebo orange juice ***
2. Placebo soup + vitamin D2 supplement in orange juice
3. Placebo soup + placebo orange juice
50.0 # 76.7 # -28.9 #

Fortified bread

Natri et al., 2006 [67]
10 microgram (27.3 ng/100 g)
3 weeks, February-March 25-45 years, healthy females (25(OH)D < 58.1 nmol/L) n = 41
1. Vitamin D3-fortified wheat bread (85 g)
2. Vitamin D3-fortified rye bread
3. Regular wheat bread + vitamin D3 supplement
4. Regular wheat bread
65.0 ### 59.0 ### 78.0 ### -1.2 ###

* equivalent to a daily dose; ** ingested in one weekly dose; *** all portions served once a week;
# measurement at the end of the intervention; ## combined placebo
groups; ### estimated baseline 25(OH)D; A all orange juice in this study contained 350 mg Ca/236.6 mL.

In the current literature the most commonly used vehicles for vitamin D fortification are dairy products (milk, cheese and yogurt). Two long term studies (24 months) used milk fortified with vitamin D3 to deliver 10 microgram or greater per day [64,65]. In both studies, 25(OH)D concentrations increased for the supplemented group compared to baseline; however, the relative increases varied between the studies (25.0% vs. 7.4%) which may be as a result of very different study populations. It should be noted that in the study by Daly and colleagues a 20% decrease in serum 25(OH)D was reported for the control group. In general, compliance was good, with only a small number of dropouts reported despite the necessity to consume a large volume of milk (400 mL/day). Both studies concluded that vitamin D was bioavailable and improved 25(OH)D status and markers of bone turnover. Similarly, daily intake of 25 microgram of vitamin D3 in a yogurt drink with or without added calcium significantly increased serum 25(OH)D3 concentrations after 12 weeks by 75.0% and 67.6%, respectively, compared to a decrease of 10.6% in the control group. In addition, the glycaemic status in diabetic patients was improved [68]. Acceptance of the drink was high among volunteers and no adverse effects were observed.

Fortification of cheese with vitamin D has been examined in two separate eight-week studies showing diverse results. In the study by Johnson and colleagues, consumption of fortified cheese (15 microgram vitamin D) resulted in an 8.7% decrease in their serum 25(OH)D [66]. This was an unexpected finding that the authors attribute to a higher baseline value of 25(OH)D in the supplemented group. Additionally no changes in serum PTH were found. The second study by Wagner et al. showed an approximate 120% increase in serum 25(OH)D concentration after a weekly dose of 700 microgram vitamin D fortified cheese [71]. In this study serum PTH decreased and there were no changes in serum calcium concentration; additionally no dropouts or adverse side effects observed were reported. However, in the study by Johnson and colleagues there were five dropouts in the cheese group: the reasons for withdrawal included gastrointestinal problems, a dislike for the saltiness of the cheese and medical advice [66]. Indeed, as cheese typically contains relatively high levels of salt and saturated fat, a portion size closer to that usually recommended for cheese (30 g) rather than what was used by Johnson et al. (85 g) may have been more appropriate [66]. Overall, cheese could be a feasible food for the improvement of vitamin D status, but a more realistic amount and vitamin D dose should be used in future studies. To summarise, vitamin D was bioavailable from all studied dairy products; enhanced milk and yogurt or a very high weekly intake of enhanced cheese improved vitamin D status, whilst consumption of 15 microgram of vitamin D in cheese was insufficient to produce a significant increase in serum 25(OH)D.

Due to lactose intolerance or low dairy product consumption in some countries, new foods should be considered as possible vehicles for enhancement with vitamin D. Two RCTs using orange juice fortified with 25 microgram of vitamin D showed a positive effect on serum 25(OH)D concentration [63,69]. In the study of Tangpricha et al., 25(OH)D increased by 150% after 12 weeks supplementation [69]. The second study reported that ingestion of 25 microgram vitamin D2 and D3 fortified orange juice for 11 weeks resulted in a rise in serum 25(OH)D concentration by 67.1% and 71.5%, respectively [63]. Serum PTH decreased only in the first study, while calcium concentration remained stable in both interventions. No adverse effects were reported in either study. The fortification of orange juice with vitamin D seems a promising method of boosting serum 25(OH)D concentration. However, replication of the above studies using a daily dose in line with the RDA for vitamin D is warranted.

Another non-dairy vitamin D enhanced product that has received attention recently is mushrooms. Vitamin D2 from wild grown mushrooms was well absorbed and bioavailable in humans [72]. This study used wild and not enhanced mushrooms, and therefore has not been included in this review that focuses on enhanced vitamin D foods. However, a recent paper has investigated the effect of a weekly dose of 700 microgram of vitamin D from vitamin D2-enhanced mushrooms in a soup [70] and reported a 50% increase in serum 25(OH)D concentration. There were no changes in serum PTH and calcium concentration and no adverse effects were observed. Additionally, the soup was well tolerated by volunteers. The limitations of this study include the low number of subjects and the high vitamin D dose ingested weekly. Nonetheless, this study demonstrated that vitamin D from mushrooms was bioavailable and shows promise as an alternative to supplements in maintaining serum 25(OH)D concentration.

Bread fortified with vitamin D could also serve as a good source of vitamin D due to its common consumption. Promising results were obtained following daily consumption of 125 microgram of vitamin D3 enhanced bread for 12 months in older adults [73], however the details of the study are not included in Table 2 as it was a single-arm design. To date, only one RCT has investigated the effect of supplementation of wheat and rye bread with 10 microgram of vitamin D3 per daily 85 g portion (equivalent to four thin slices) [67]. In this study serum 25(OH)D increased by approximately 60% after 3 weeks in both groups that received fortified bread. No differences in serum PTH were found, while calcium concentration decreased in the fortified rye bread group. Three subjects dropped out of the study due to difficulties with compliance. In conclusion, fortified bread increased serum 25(OH)D concentration, with rye and wheat bread equally effective.

Finally, the aforementioned RCTs show the potential of a number of vitamin D enhanced foods to increase vitamin D status. Overall, fortification was the principal strategy adopted but the potential of UV light enhanced mushrooms to boost vitamin D status was more recently explored. A comparison between the studies is difficult to perform because of variability in the population characteristics (i.e., age, gender), dose of vitamin D provided, intervention period and methods used for 25(OH)D determination [74,75]. The majority of the studies provided a daily supply of the foods whilst two used a weekly dose. However, the impact of the frequency of dose has not been studied in relation to any of the above foods. With respect to intake of vitamin D supplements it has been demonstrated that the frequency of intake has an impact on 25(OH)D serum concentrations [76], illustrating the imperative need to study this in the vitamin D enhanced foods. Overall, in the enhanced food a higher dose was more effective in increasing 25(OH)D concentration; however, for short term studies (3 weeks) the increase seemed to be independent of dose. The majority of the foods in the selected RCTs were well tolerated by the study subjects. After the ingestion of different vitamin D fortified/enriched foods, PTH decreased or did not change, while serum calcium concentration remained stable or in the case of the rye bread group decreased, highlighting no safety risks with the use of these vitamin D enhanced products. Milk, yogurt, orange juice, mushrooms and bread proved to be good matrices for supplementation with vitamin D at the level of 10 microgram (milk, bread), 25 microgram (orange juice and yogurt) or or 100 microgram (mushrooms). More studies are needed to assess the effectiveness of vitamin D fortified cheese, with the use of a more feasible serving size. Finally, more RCTs, using these foods, should be conducted to investigate the link between vitamin D and other disease markers beyond those related to bone health and vitamin D status.

4. Vitamin D2 and D3 Bioavailability

Until relatively recently it was widely believed, based upon anti-rachitic findings, that vitamin D2 and D3 were equipotent and could be considered interchangeably. However, evidence is accumulating to suggest the two forms may vary in their bioefficacy and the importance of this must be considered when discussing potential food matrices. A number of recent studies addressing the potency of the two calciferols have found D3 to be more effective in raising serum 25(OH)D levels. In a group of vitamin D insufficient hip fracture patients, a daily capsule of 25 microgram D3 was more effective than an equal dose of D2 in increasing total serum 25(OH)D [77]. Also in older populations, 15 microgram daily or 1250 microgram monthly of capsular vitamin D3 increased serum 25(OH)D more efficiently than an equal dose of D2 [78], as has also been shown with a single 7500 microgram dose of oral or intramuscular D3 [79]. The increased ability of a weekly capsule of 1250 microgram of D3 for 12 weeks to maintain serum 25(OH)D when compared with equimolar D2 has also been shown in healthy adults [80]. These findings support those of a number of earlier studies also addressing this topic [81,82].

The above findings were not replicated in a number of recent studies in which D2 and D3 were found to be equally effective in increasing serum 25(OH)D when administered to healthy adults as capsules [63,83] or in orange juice [63] for 11 weeks, or in a high-dose liquid suspension to vitamin D deficient infants for 6 weeks [84]. Despite this, the general consensus that is beginning to emerge is that vitamin D3 is the more potent form. However, it is difficult to directly compare the results of the discussed studies given the many confounding factors between them. Most notable in this regard are the different methods employed to analyze serum 25(OH)D, the differing baseline serum levels, the variance in age groups studied, the doses administered, and the duration of supplementation. Overall, more studies specifically designed to address this question are needed before the results can be translated into a public health message.

5. Regulation Surrounding the Sale of Foods Enhanced with Vitamin D

From a regulatory perspective, the vitamin D enhanced foods mentioned in this review have typically fallen into two categories
(i) fortified foods and
(ii) foods that have used technologies to enhance naturally occurring vitamin D levels.
However, prior to sale on a commercial scale, there are a series of steps which potential manufacturers must consider.
Globally these can vary depending on the region (e.g., Europe, US) and also between countries (e.g., with respect to fortification policies).
This review will now briefly outline some of the regulations that must be considered in the EU.

For fortified foods, addition of both ergocalciferol and cholecalciferol is permitted but consideration must be given to dietary intake from other sources and safe upper levels of intake before agreeing on the levels of addition [85]. At present the IOM has defined 100 microgram/day (4,000 IU) as an upper level for vitamin D intake [29]. Regarding foods which exploit new technologies to enhance naturally occurring levels (as in the case of vitamin D enhanced mushrooms, which were irradiated by UV light), if they are not currently widely consumed within the EU, they would be considered as a "novel food" and subject to approval as "safe for use" by the competent authority in the applicant country and also in other Member States [86].

Thereafter, both categories of food are subject to general food law labelling.
Currently, in the EU vitamins and minerals can be declared on food only if they are present in a "significant" amount, i.e., >15% of Recommended Daily Allowance (RDA) [87].
According to current legislation in relation to vitamin D, food containing >0.75 microgram vitamin D/100 g (100 mL) product can be claimed as a "source" of vitamin D, whereas to be declared as "high" in vitamin D, it has to contain at least twice the value of a "source" of vitamin D, i.e., >1.5 microgram/100 g (100 mL) or 30% RDA [88,89]. For those manufacturers who wish to make further nutrition and health claims, specific procedures and conditions for use are applicable to each claim and are outlined in the appropriate regulations [88].
In brief, there are three categories of permitted health claims

• (i) article 13 general function claims;
• (ii) article 13.5 claims based on newly developed scientific evidence; and
• (iii) article 14 claims referring to the reduction of a risk factor in the development of a disease and children's development and health.

Currently, approved vitamin D health claims relate to vitamin D and bone and teeth health, absorption and utilisation of calcium and phosphorus, normal function of the immune system, cell division, normal growth and development of bone in children, as well as claims referring to the relationship between calcium and vitamin D and the reduction of bone loss in elderly women [90-94]. However, if a vitamin D-enhanced product wishes to bear any of the above claims, it must conform to the aforementioned conditions for use and to as yet unspecified nutrient profiles [87,88].

As mentioned, we conducted food modelling to simulate the intake of vitamin D from enhanced foods in the Irish adult population. Initially the effect of consumption of orange juice enhanced with 10.5 microgram vitamin D per 100 g on mean daily intakes was investigated. Consumption of such orange juice would increase mean intakes of vitamin D from 4.7 microgram to 7.9 microgram per day. In another simulation designed to mimic mandatory fortification policies, in tandem with the orange juice, milk was enhanced at a conservative level of 1 microgram/100 g. In this scenario intakes increased to 9.9 microgram/day, however, it also resulted in 1% of the population consuming >50 microgram vitamin D, thereby reiterating the need to be cognisant of extreme consumers. On the other hand, any mandatory fortification policies would need to be aware of non-consumers of the particular enhanced food group: to this end it may be prudent to introduce fortification at low levels in a number of foods.

6. Conclusions

Overall, the evidence that enhanced vitamin D foods can improve vitamin D status is convincing. A range of enhanced foods were shown to be effective in increasing circulating 25(OH)D concentrations with no signs of adverse effects. Further research and development in such foods is warranted as they have the potential to provide a means to address the vitamin D deficiency pandemic.

Acknowledgments

We would like to acknowledge Monaghan Mushrooms for support of research in our laboratories.

Conflict of Interest

The authors declare no conflict of interest.

References

• 1. Norman, A.W.; Bouillon, R. Vitamin D nutritional policy needs a vision for the future. Exp. Biol. Med. (Maywood) 2010, 235, 1034-1045.
• 2. Haussler, M.R.; Haussler, C.A.; Jurutka, P.W.; Thompson, P.D.; Hsieh, J.C.; Remus, L.S.; Selznick, S.H.; Whitfield, G.K. The vitamin D hormone and its nuclear receptor: Molecular actions and disease states. J. Endocrinol. 1997, 154 (Suppl.), S57-S73.
• 3. Hess, A.F.; Unger, L.J. The cure of infantile rickets by sunlight. JAMA 1922, 78, 29-31.
• 4. Bhan, A.; Rao, A.D.; Rao, D.S. Osteomalacia as a result of vitamin D deficiency. Endocrinol. Metab. Clin. N. Am. 2010, 39, 321-331.
• 5. Avenell, A.; Gillespie, W.J.; Gillespie, L.D.; O'Connell, D. Vitamin D and vitamin D analogues for preventing fractures associated with involutional and post-menopausal osteoporosis. Cochrane Database Syst. Rev. 2009, CD000227.
• 6. Lips, P.; Bouillon, R.; van Schoor, N.M.; Vanderschueren, D.; Verschueren, S.; Kuchuk, N.; Milisen, K.; Boonen, S. Reducing fracture risk with calcium and vitamin D. Clin. Endocrinol. (Oxf.) 2010, 73, 277-285.
• 7. Holick, M.F. Vitamin D: Evolutionary, physiological and health perspectives. Curr. Drug Targets 2011, 12, 4-18.
• 8. Chu, K.J.; Lai, E.C.; Yao, X.P.; Zhang, H.W.; Lau, W.Y.; Fu, X.H.; Lu, C.D.; Shi, J.; Cheng, S.Q. Vitamin analogues in chemoprevention of hepatocellular carcinoma after resection or ablation—a systematic review and meta-analysis. Asian J. Surg. 2010, 33, 120-126.
• 9. Zhang, H.L.; Wu, J. Role of vitamin D in immune responses and autoimmune diseases, with emphasis on its role in multiple sclerosis. Neurosci. Bull. 2010, 26, 445-454.
• 10. Grant, W.B. An estimate of the global reduction in mortality rates through doubling vitamin D levels. Eur. J. Clin. Nutr. 2011, 65, 1016-1026.
• 11. Grant, W.B.; Schwalfenberg, G.K.; Genuis, S.J.; Whiting, S.J. An estimate of the economic burden and premature deaths due to vitamin D deficiency in Canada. Mol. Nutr. Food Res. 2010, 54, 1172-1181.
• 12. Ginde, A.A.; Scragg, R.; Schwartz, R.S.; Camargo, C.A. Prospective study of serum 25-hydroxyvitamin D level, cardiovascular disease mortality, and all-cause mortality in older US adults. J. Am. Geriatr. Soc. 2009, 57, 1595-1603.
• 13. Dobnig, H.; Pilz, S.; Scharnagl, H.; Renner, W.; Seelhorst, U.; Wellnitz, B.; Kinkeldei, J.; Boehm, B.O.; Weihrauch, G.; Maerz, W. Independent association of low serum 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D levels with all-cause and cardiovascular mortality. Arch. Intern. Med. 2008, 168, 1340-1349.
• 14. Holick, M.F.; Chen, T.C. Vitamin D deficiency: A worldwide problem with health consequences. Am. J. Clin. Nutr. 2008, 87, 1080S-1086S.
• 15. Mithal, A.; Wahl, D.A.; Bonjour, J.P.; Burckhardt, P.; Dawson-Hughes, B.; Eisman, J.A.; El-Hajj Fuleihan, G.; Josse, R.G.; Lips, P.; Morales-Torres, J. Global vitamin D status and determinants of hypovitaminosis D. Osteoporos. Int. 2009, 20, 1807-1820.
• 16. Zhang, R.; Naughton, D.P. Vitamin D in health and disease: Current perspectives. Nutr. J. 2010, 9, 65.
• 17. Lips, P. Vitamin D status and nutrition in europe and asia. J. Steroid Biochem. Mol. Biol. 2007, 103, 620-625.
• 18. Hill, T.R.; Flynn, A.; Kiely, M.; Cashman, K.D. Prevalence of suboptimal vitamin D status in young, adult and elderly Irish subjects. Ir. Med. J. 2006, 99, 48-49.
• 19. Atherton, K.; Berry, D.J.; Parsons, T.; Macfarlane, G.J.; Power, C.; Hypponen, E. Vitamin D and chronic widespread pain in a white middle-aged british population: Evidence from a cross-sectional population survey. Ann. Rheum. Dis. 2009, 68, 817-822.
• 20. Hill, T.; Collins, A.; O'Brien, M.; Kiely, M.; Flynn, A.; Cashman, K.D. Vitamin D intake and status in Irish postmenopausal women. Eur. J. Clin. Nutr. 2005, 59, 404-410.
• 21. Lapatsanis, D.; Moulas, A.; Cholevas, V.; Soukakos, P.; Papadopoulou, Z.L.; Challa, A. Vitamin D: A necessity for children and adolescents in Greece. Calcif. Tissue Int. 2005, 77, 348-355.
• 22. Isaia, G.; Giorgino, R.; Rini, G.B.; Bevilacqua, M.; Maugeri, D.; Adami, S. Prevalence of hypovitaminosis D in elderly women in Italy: Clinical consequences and risk factors. Osteoporos. Int. 2003, 14, 577-582.
• 23. Langlois, K.; Greene-Finestone, L.; Little, J.; Hidiroglou, N.; Whiting, S. Vitamin D status of Canadians as measured in the 2007 to 2009 Canadian health measures survey. Health Rep. 2010, 21, 47-55.
• 24. Looker, A.C.; Pfeiffer, C.M.; Lacher, D.A.; Schleicher, R.L.; Picciano, M.F.; Yetley, E.A. Serum 25-hydroxyvitamin D status of the us population: 1988-1994 compared with 2000-2004. Am. J. Clin. Nutr. 2008, 88, 1519-1527.
• 25. Forrest, K.Y.Z.; Stuhldreher, W.L. Prevalence and correlates of vitamin D deficiency in US adults. Nutr. Res. 2011, 31, 48-54.
• 26. Ashwell, M.; Stone, E.M.; Stolte, H.; Cashman, K.D.; Macdonald, H.; Lanham-New, S.; Hiom, S.; Webb, A.; Fraser, D. UK food standards agency workshop report: An investigation of the relative contributions of diet and sunlight to vitamin D status. Br. J. Nutr. 2010, 104, 603-611.
• 27. Miller, G. Report from the Chair of the Scottish Food Advisory Committee; Info10/12/03; Food Standards Agency: London, UK, 2010.
• 28. Doets, E.L.; de Wit, L.S.; Dhonukshe-Rutten, R.A.; Cavelaars, A.E.; Raats, M.M.; Timotijevic, L.; Brzozowska, A.; Wijnhoven, T.M.; Pavlovic, M.; Totland, T.H.; et al. Current micronutrient recommendations in Europe: Towards understanding their differences and similarities. Eur. J. Nutr. 2008, 47, S17-S40.
• 29. Institute of Medicine. Dietary Reference Intakes for Calcium and Vitamin D; The National Academies Press: Washington, DC, USA, 2011.
• 30. Freisling, H.; Fahey, M.T.; Moskal, A.; Ocke, M.C.; Ferrari, P.; Jenab, M.; Norat, T.; Naska, A.; Welch, A.A.; Navarro, C.; et al. Region-specific nutrient intake patterns exhibit a geographical gradient within and between European countries. J. Nutr. 2010, 140, 1280-1286.
• 31. Serra-Majem, L.; Ribas-Barba, L.; Salvador, G.; Jover, L.; Raido, B.; Ngo, J.; Plasencia, A. Trends in energy and nutrient intake and risk of inadequate intakes in Catalonia, Spain (1992-2003). Public Health Nutr. 2007, 10, 1354-1367.
• 32. Sette, S.; Le Donne, C.; Piccinelli, R.; Arcella, D.; Turrini, A.; Leclercq, C. The third Italian national food consumption survey, INRAN-SCAI 2005-06—Part 1: Nutrient intakes in Italy. Nutr. Metab. Cardiovasc. Dis. 2011, 21, 922-932.
• 33. The National Findiet 2007 Survey; Paturi, M., Tapanainen, H., Reinivuo, H., Pietinen, P., Eds.; National Public Health Institute: Helsinki, Finland, 2008.
• 34. Irish Universities Nutrition Alliance. National Adult Nutrition Survey; Irish Universities Nutrition Alliance: Dublin, Ireland, 2011.
• 35. Bailey, R.L.; Dodd, K.W.; Goldman, J.A.; Gahche, J.J.; Dwyer, J.T.; Moshfegh, A.J.;
• Sempos, C.T.; Picciano, M.F. Estimation of total usual calcium and vitamin D intakes in the United States. J. Nutr. 2010, 140, 817-822.
• 36. Vatanparast, H.; Calvo, M.S.; Green, T.J.; Whiting, S.J. Despite mandatory fortification of staple foods, vitamin D intakes of Canadian children and adults are inadequate. J. Steroid Biochem. Mol. Biol. 2010, 121, 301-303.
• 37. Nanri, A.; Foo, L.H.; Nakamura, K.; Hori, A.; Poudel-Tandukar, K.; Matsushita, Y.; Mizoue, T. Serum 25-hydroxyvitamin D concentrations and season-specific correlates in Japanese adults. J. Epidemiol. 2011, 21, 346-353.
• 38. Calvo, M.S.; Whiting, S.J.; Barton, C.N. Vitamin D intake: A global perspective of current status. J. Nutr. 2005, 135, 310-316.
• 39. Henderson, L.; Irving, K.; Gregory, J.; Bates, C.J.; Prentice, A.; Perks, J.; Swan, G.; Farron, M. The National Diet and Nutrition Survey: Adults Aged 19 to 64 Years—Vitamin and Mineral Intake and Urinary Analysis; Office for National Statistics: Newport, UK, 2003; Volume 3.
• 40. Park, Y.K.; Barton, C.N.; Calvo, M.S. Dietary contributions to serum 25(OH) vitamin D levels [25(OH)D] differ in black and white adults in the United States: Results from NHANES III. J. Bone Miner. Res. 2001, 16, F281.
• 41. Kenkyukai, K.E.J. The National Health and Nutrition Survey in Japan; Daiichi-shuppan: Tokyo, Japan, 2010.
• 42. Nakamura, K.; Nashimoto, M.; Okuda, Y.; Ota, T.; Yamamoto, M. Fish as a major source of vitamin D in the Japanese diet. Nutrition 2002, 18, 415-416.
• 43. Lu, Z.; Chen, T.C.; Zhang, A.; Persons, K.S.; Kohn, N.; Berkowitz, R.; Martinello, S.; Holick, M.F. An evaluation of the vitamin D3 content in fish: Is the vitamin D content adequate to satisfy the dietary requirement for vitamin D? J. SteroidBiochem. Mol. Biol. 2007, 103, 642-644.
• 44. Food Standards Agency. Mccance and Widdowson's the Composition of Foods Sixth Summary Edition, 6th revised ed.; Royal Society of Chemistry: Cambridge, UK, 2002.
• 45. U.S. Department of Agriculture A.R.S. USDA national nutrient database for standard reference. Available online: http://www.ars.usda.gov/nutrientdata (accessed on 15 October 2011).
• 46. Mattila, P.; Ronkainen, R.; Lehikoinen, K.; Piironen, V. Effect of household cooking on the vitamin D content in fish, eggs, and wild mushrooms. J. Food Compos. Anal. 1999, 12, 153-160.
• 47. Mattila, P.H.; Piironen, V.I.; Uusirauva, E.J.; Koivistoinen, P.E. Vitamin-D contents in edible mushrooms. J. Agric. Food Chem. 1994, 42, 2449-2453.
• 48. Rangel-Castro, J.I.; Staffas, A.; Danell, E. The ergocalciferol content of dried pigmented and albino Cantharellus cibarius fruit bodies. Mycol. Res. 2002, 106, 70-73.
• 49. Monterey Mushrooms Mushroom nutritional chart. Available online: http://www. montereymushrooms.com/wp-content/uploads/2011/06/mushroom-nutritional-chart.gif (accessed on 15 October 2011).
• 50. Avonmore Whole super milk. Available online: http://www.avonmoresupermilk.ie/range/ wholesupermilk.html (accessed on 15 October 2011).
• 51. Wonderbread Nutritional facts. Available online: http://www.wonderbread.com/#/products/ white_breads/classic_white (accessed on 15 October 2011)
• 52. FAO/WHO. Codex Alimentarius, 2nd ed.; FAO/WHO: Roma, Italy, 1994; Volume 4.
• 53. US Food and Drug Administration. Title 21: Food and Drug Administration. In Code of Federal Regulations; US Government Printing Office: Washington, DC, USA, 2004; Volume 2, pp. 100-169.
• 54. Lehtonen-Veromaa, M.; Mottonen, T.; Leino, A.; Heinonen, O.J.; Rautava, E.; Viikari, J. Prospective study on food fortification with vitamin D among adolescent females in Finland: Minor effects. Br. J. Nutr. 2008, 100, 418-423.
• 55. Ko, J.A.; Lee, B.H.; Lee, J.S.; Park, H.J. Effect of UV-B exposure on the concentration of vitamin D2 in sliced shiitake mushroom (Lentinus edodes) and white button mushroom (Agaricus bisporus). J. Agric. Food Chem. 2008, 56, 3671-3674.
• 56. Koyyalamudi, S.R.; Jeong, S.C.; Song, C.H.; Cho, K.Y.; Pang, G. Vitamin D2 formation and bioavailability from Agaricus bisporus button mushrooms treated with ultraviolet irradiation. J. Agric. Food Chem. 2009, 57, 3351-3355.
• 57. Roberts, J.S.; Teichert, A.; McHugh, T.H. Vitamin D2 formation from post-harvest UV-B treatment of mushrooms (Agaricus bisporus) and retention during storage. J. Agric. Food Chem. 2008, 56, 4541-4544.
• 58. Wilborn, B.S.; Kerth, C.R.; Owsley, W.F.; Jones, W.R.; Frobish, L.T. Improving pork quality by feeding supranutritional concentrations of vitamin D3. J. Anim. Sci. 2004, 82, 218-224.
• 59. Jakobsen, J.; Maribo, H.; Bysted, A.; Sommer, H.M.; Hels, O. 25-hydroxyvitamin D3 affects vitamin D status similar to vitamin D3 in pigs—but the meat produced has a lower content of vitamin D. Br. J. Nutr. 2007, 98, 908-913.
• 60. Graff, I.E.; H0Ie, S.; Totland, G.K.; Lie, 0. Three different levels of dietary vitamin D3 fed to first-feeding fry of Atlantic salmon (Salmo salar L.): Effect on growth, mortality, calcium content and bone formation. Aquac. Nutr. 2002, 8, 103-111.
• 61. Mattila, P.; Valaja, J.; Rossow, L.; Venalainen, E.; Tupasela, T. Effect of vitamin D2- and D3-enriched diets on egg vitamin D content, production, and bird condition during an entire production period. Poult. Sci. 2004, 83, 433-440.
• 62. Mattila, P.; Lehikoinen, K.; Kiiskinen, T.; Piironen, V. Cholecalciferol and 25-hydroxycholecalciferol content of chicken egg yolk as affected by the cholecalciferol content of feed. J. Agric. Food Chem. 1999, 47, 4089-4092.
• 63. Biancuzzo, R.M.; Young, A.; Bibuld, D.; Cai, M.H.; Winter, M.R.; Klein, E.K.; Ameri, A.; Reitz, R.; Salameh, W.; Chen, T.C.; et al. Fortification of orange juice with vitamin D2 or vitamin D3 is as effective as an oral supplement in maintaining vitamin D status in adults. Am. J. Clin. Nutr. 2010, 91, 1621-1626.
• 64. Chee, W.S.; Suriah, A.R.; Chan, S.P.; Zaitun, Y.; Chan, Y.M. The effect of milk supplementation on bone mineral density in postmenopausal Chinese women in Malaysia. Osteoporos. Int. 2003, 14, 828-834.
• 65. Daly, R.M.; Brown, M.; Bass, S.; Kukuljan, S.; Nowson, C. Calcium- and vitamin D3-fortified milk reduces bone loss at clinically relevant skeletal sites in older men: A 2-year randomized controlled trial. J. Bone Miner. Res. 2006, 21, 397-405.
• 66. Johnson, J.L.; Mistry, V.V.; Vukovich, M.D.; Hogie-Lorenzen, T.; Hollis, B.W.; Specker, B.L. Bioavailability of vitamin D from fortified process cheese and effects on vitamin D status in the elderly. J. Dairy Sci. 2005, 88, 2295-2301.
• 67. Natri, A.M.; Salo, P.; Vikstedt, T.; Palssa, A.; Huttunen, M.; Karkkainen, M.U.; Salovaara, H.; Piironen, V.; Jakobsen, J.; Lamberg-Allardt, C.J. Bread fortified with cholecalciferol increases the serum 25-hydroxyvitamin D concentration in women as effectively as a cholecalciferol supplement. J. Nutr. 2006, 136, 123-127.
• 68. Nikooyeh, B.; Neyestani, T.R.; Farvid, M.; Alavi-Majd, H.; Houshiarrad, A.; Kalayi, A.; Shariatzadeh, N.; Gharavi, A.; Heravifard, S.; Tayebinejad, N.; et al. Daily consumption of vitamin D- or vitamin D + calcium-fortified yogurt drink improved glycemic control in patients with type 2 diabetes: A randomized clinical trial. Am. J. Clin. Nutr. 2011, 93, 764-771.
• 69. Tangpricha, V.; Koutkia, P.; Rieke, S.M.; Chen, T.C.; Perez, A.A.; Holick, M.F. Fortification of orange juice with vitamin D: A novel approach for enhancing vitamin D nutritional health. Am. J. Clin. Nutr. 2003, 77, 1478-1483.
• 70. Urbain, P.; Singler, F.; Ihorst, G.; Biesalski, H.K.; Bertz, H. Bioavailability of vitamin D2 from UV-B-irradiated button mushrooms in healthy adults deficient in serum 25-hydroxyvitamin D: A randomized controlled trial. Eur. J. Clin. Nutr. 2011, 65, 965-971.
• 71. Wagner, D.; Sidhom, G.; Whiting, S.J.; Rousseau, D.; Vieth, R. The bioavailability of vitamin D from fortified cheeses and supplements is equivalent in adults. J. Nutr. 2008, 138, 1365-1371.
• 72. Outila, T.A.; Mattila, P.H.; Piironen, V.I.; Lamberg-Allardt, C.J. Bioavailability of vitamin D from wild edible mushrooms (Cantharellus tubaeformis) as measured with a human bioassay. Am. J. Clin. Nutr. 1999, 69, 95-98.
• 73. Mocanu, V.; Stitt, P.A.; Costan, A.R.; Voroniuc, O.; Zbranca, E.; Luca, V.; Vieth, R. Long-term effects of giving nursing home residents bread fortified with 125 [jg (5000 IU) vitamin D3 per daily serving. Am. J. Clin. Nutr. 2009, 89, 1132-1137.
• 74. Lai, J.K.; Lucas, R.M.; Banks, E.; Ponsonby, A.L. Variability in vitamin D assays impairs clinical assessment of vitamin D status. Intern. Med. J. 2011, doi:10.1111/j.1445-5994.2011.02471.x.
• 75. Snellman, G.; Melhus, H.; Gedeborg, R.; Byberg, L.; Berglund, L.; Wernroth, L.; Michaelsson, K. Determining vitamin D status: A comparison between commercially available assays. PLoS One 2010, 5, e11555.
• 76. Chel, V.; Wijnhoven, H.A.; Smit, J.H.; Ooms, M.; Lips, P. Efficacy of different doses and time intervals of oral vitamin D supplementation with or without calcium in elderly nursing home residents. Osteoporos. Int. 2008, 19, 663-671.
• 77. Glendenning, P.; Chew, G.T.; Seymour, H.M.; Gillett, M.J.; Goldswain, P.R.; Inderjeeth, C.A.; Vasikaran, S.D.; Taranto, M.; Musk, A.A.; Fraser, W.D. Serum 25-hydroxyvitamin D levels in vitamin D-insufficient hip fracture patients after supplementation with ergocalciferol and cholecalciferol. Bone 2009, 45, 870-875.
• 78. Binkley, N.; Gemar, D.; Engelke, J.; Gangnon, R.; Ramamurthy, R.; Krueger, D.; Drezner, M.K. Evaluation of ergocalciferol or cholecalciferol dosing, 1600 IU daily or 50,000 IU monthly in older adults. J. Clin. Endocrinol. Metab. 2011, 96, 981-988.
• 79. Romagnoli, E.; Mascia, M.L.; Cipriani, C.; Fassino, V.; Mazzei, F.; D'Erasmo, E.; Carnevale, V.; Scillitani, A.; Minisola, S. Short and long-term variations in serum calciotropic hormones after a single very large dose of ergocalciferol (vitamin D2) or cholecalciferol (vitamin D3) in the elderly. J. Clin. Endocrinol. Metab. 2008, 93, 3015-3020.
• 80. Heaney, R.P.; Recker, R.R.; Grote, J.; Horst, R.L.; Armas, L.A. Vitamin D3 is more potent than vitamin D2 in humans. J. Clin. Endocrinol. Metab. 2010, 96, E447-E452.
• 81. Armas, L.A.G.; Hollis, B.W.; Heaney, R.P. Vitamin D2 is much less effective than vitamin D3 in humans. J. Clin. Endocrinol. Metab. 2004, 89, 5387-5391.
• 82. Leventis, P.; Kiely, P.D. The tolerability and biochemical effects of high-dose bolus vitamin D2 and D3 supplementation in patients with vitamin D insufficiency. Scand. J. Rheumatol. 2009, 38, 149-153.
• 83. Holick, M.F.; Biancuzzo, R.M.; Chen, T.C.; Klein, E.K.; Young, A.; Bibuld, D.; Reitz, R.; Salameh, W.; Ameri, A.; Tannenbaum, A.D. Vitamin D2 is as effective as vitamin D3 in maintaining circulating concentrations of 25-hydroxyvitamin D. J. Clin. Endocrinol. Metab. 2008, 93, 677-681.
• 84. Gordon, C.M.; Williams, A.L.; Feldman, H.A.; May, J.; Sinclair, L.; Vasquez, A.; Cox, J.E. Treatment of hypovitaminosis D in infants and toddlers. J. Clin. Endocrinol. Metab. 2008, 93, 2716-2721.
• 85. Commission Regulation (EC) No 1170/2009 of 30 November 2009 Amending Directive 2002/46/EC of the European Parliament and of Council and Regulation (EC) No 1925/2006 of the European Parliament and of the Council as Regards the Lists of Vitamin and Minerals and Their Forms that Can Be Added to Foods, Including Food Supplements; Official Journal of the European Union L 314; European Union: Luxembourg, Luxembourg, 2009.
• 86. Regulation (EC) No 258/97 of the European Parliament and of the Council of 27 January 1997 Concerning Novel Foods and Novel Food Ingredients; Official Journal of the European Union L 043; European Union: Luxembourg, Luxembourg, 1997.
• 87. Council Directive 90/496/EEC on Nutrition Labelling for Foodstuffs (90/496/EEC) as Amended by Commission Directives 2003/120/EC and 2008/100/EC; Official Journal of the European Union L 276; European Union: Luxembourg, Luxembourg, 1990.
• 88. Regulation (EC) No 1924/2006 of the European Parliament and of the Council of 20 December 2006 on Nutrition and Health Claims Made on Foods as Amended by Regulation (EC) No 107/2008 and Regulation (EC) No 109/2008; Official Journal of the European Union OJ L 404; European Union: Luxembourg, Luxembourg, 2006.
• 89. Annex 1. In Commission Directive 2008/100/EC of 28 October 2008 Amending Council Directive 90/496/EEC on Nutrition Labelling for Foodstuffs as Regards Recommended Daily Allowances, Energy Conversion Factors and Definitions; Official Journal of the European Union OJ L 285; European Union: Luxembourg, Luxembourg, 2008.
• 90. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Scientific opinion on the substantiation of health claims related to vitamin D and maintenance of bone and teeth (ID 150, 151, 158), absorption and utilisation of calcium and phosphorus and maintenance of normal blood calcium concentrations (ID 152, 157), cell division (ID 153), and thyroid function (ID 156) pursuant to article 13(1) of regulation (EC) No 1924/2006. EFSA J. 2009, 7, doi:10.2903/j.efsa.2009.1227.
• 91. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Scientific opinion on the substantiation of health claims related to vitamin D and normal function of the immune system and inflammatory response (ID 154, 159), maintenance of normal muscle function (ID 155) and maintenance of normal cardiovascular function (ID 159) pursuant to article 13(1) of regulation (EC) No 1924/2006. EFSA J. 2010, 8, doi:10.2903/j.efsa.2010.1468.
• 92. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Vitamin D and bone growth—Scientific substantiation of a health claim related to vitamin D and bone growth pursuant to Article 14 of Regulation (EC) No 1924/20061—Scientific Opinion of the Panel on Dietetic Products, Nutrition and Allergies. EFSA J. 2008, 6, doi:10.2903/j.efsa.2008.827.
• 93. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Scientific opinion in relation to the authorisation procedure for health claims on calcium and vitamin D and the reduction of the risk of osteoporotic fractures by reducing bone loss pursuant to article 14 of regulation (EC) No 1924/2006. EFSA J. 2010, 8, doi:10.2903/j.efsa.2010.1609.
• 94. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Calcium + vitamin D3 chewing tablets and bone loss—Scientific substantiation of a health claim related to calcium plus vitamin D3 chewing tablets and reduction of the risk of osteoporotic fractures by reducing bone loss pursuant to Article 14 of Regulation (EC) No 1924/2006—Scientific Opinion of the Panel on Dietetic Products, Nutrition and Allergies. EFSA J. 2009, 7, doi:10.2903/j.efsa.2009.1180.