Vitamin D in the blood encounters many restrictions in getting to the cells – Sept 2020

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

Nutrients 2020, 12(9), 2788; https://doi.org/10.3390/nu12092788
by Magdalena Kowalówka 1OrcID,Anna K. Główka 1OrcID,Marta Karaźniewicz-Łada 2,* and Grzegorz Kosewski 1

• 1 Department of Bromatology, Poznan University of Medical Sciences, 42 Marcelińska Street, 60-354 Poznań, Poland
• 2 Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 6 Święcickiego Street, 60-781 Poznań, Poland

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VitaminDWiki suspects about half of the people with Vitamin D deficient diseases
   have enough Vitamin D in their blood, but not in their cells
For many it is more important to increase vitamin D and D getting to cells than to get a Vitamin D test
Genes - some of which can be improved by supplements
CYP27B1 category listing contains the following
The CYP27B1 gene activates Vitamin D in the Kidney,    Skin,    Lungs,    Brain,   Eyes   Breasts   etc.
Poor CYP27B1 is assocated with COVID, Miscarriage,   Lupus,   Alz, Parkinson, MSA,   Rickets

CYtochrome P450 family 27 subfamily B member 1    = 25-Hydroxyvitamin D3 1-alpha-hydroxylase

• 63 items in CYP27B1 category
• 343 articles in the Genetics
• 530 articles in Vitamin D Receptor
• 178 articles in Vitamin D Binding Protein
• CYP27B1 and other genes are less activated in seniors
• CYP27B causes many health problems – March 2020
• Every Parkinson’s brain had a poor CYP27B1 gene

What can be done if have a poor CYP27B1

• Larger doses of Vitamin D
• More Bio-available: Gut-friendly form, Topical form, taken with fatty meal, taken with evening meal
• Additional sources: UV
• Increase Vitamin D metabolism: additional Magnesium, Omega-3
  • All cytochrome P450 enzymes require Mg++ as a cofactor
• Increase the amount of Vitamin D in the blood that gets to cells: increase activation of VDR
  • See: Reasons for low response to vitamin D

Vitamin D blood test misses CYP27B1 and other genes

Cancers might alter CYP24A1 gene

Vitamin D Binding Protein category listing has 178 items and the following introduction

Vitamin D Binding Protein (GC) gene can decrease the bio-available Vitamin D that can get to cells,

• GC is not the only such gene - there are 3 others, all invisible to standard Vitamin D tests
• The bio-available calculation does not notice the effect of GC, CYP27B1, CYP24A1, and VDR
• The actual D getting to the cells is a function of measured D and all 4 genes
• There is >2X increase in 8+ health problems if have poor VDBP (GC)
• It appears that VDBP only blocks oral vitamin D,
  • but NOT Vitamin D from sun, UV, topical or inhaled (tissue activated)
  • A clue: - Vitamin D from UV is 2X better for MS than oral Vitamin D

Vitamin D Binding Protein has a list of health problems

Increased Risk	Health Problem
11 X	Preeclampsia
6.5X	T1D in SA Blacks
6 X	Food Allergy
5 X	PTSD
4 X, 5X	Kidney Cancer
4 X	Poor Response to Oral Vitamin D
3 X	Ear infection
2.8 X	MS
2.5 X	Severe Autism
2 X	Colorectal Cancer
2 X	Prostate Cancer -in those with dark skins
1.3 X	Infertility

Vitamin D Receptor

• Vitamin D Receptor is associated in over 58 autoimmune studies
  • Vitamin D Receptor activation can be increased by any of: Resveratrol,  Omega-3,  Magnesium,  Zinc,   Quercetin,   non-daily Vit D,  Curcumin, intense exercise,   Ginger,   Essential oils, etc Note: The founder of VitaminDWiki uses 10 of the 12 known VDR activators
• Vitamin D Receptor and Cancers
• A poor Vitamin D Receptor is associated with many cancers (oral cancer in this case) – Jan 2019
  • studies have found 50 Cancers associated with poor Vitamin D Receptor
    • Some studies found that a Cancer has "learned" how to deactivate the Vitamin D receptor
• The activation of the Vitamin D receptor decreases with age 6 studies as of Sept 2020

Additional information on the Genes in VitaminDWiki

Gene	# of pages	Location
CYP27A1	449 (Sept 2019)	Liver
*CYP2R1	826 (Sept 2019)	Liver & Tissue
CYP27B1	1430   (Sept 2019)	Kidney & Tissue
CYP24A1	28 in title - May 2022]	excrete excess

• Vitamins regulate some human genes – Feb 2020
• The genetics of vitamin D – Oct 2018
• Does survival of the less fit mean less health (poor genes may be inherited)
  • genes getting worse over generations
• 1289 genes changed with higher doses of Vitamin D - RCT Dec 2019
• Vitamin D deficiency is associated with 35 genes, only 7 of are commercially tested – Nov 2019

Cofactors (in addition to Vitamin D Receptor supplements)
Vitamin D Cofactors in a nutshell table show the importance of each

Supplement	# of studies		Increase D to tissue	How Often
Vitamin D	986+	Most people do not have enough anymore	N.A.	daily to 2 weeks
Magnesium	369	Many people need Mg Vit D consumes Mg Muscle pain, Afib	30%	Several times a day
Vitamin K	157	Hard bones, soft arteries	115%	2 day
Omega-3	409	Synergistic with Vit D Independent of Vit D	30? %	2 day
Calcium	227	Reduce or add Vit K		2 day
Zinc	90	immunity	20 ?% if poor VDR	7 day
Boron	37	plants and animals need B	+20%	7 day
Resveratrol	48	antioxidant	>100% if poor VDR	Daily
Glutathione	10+	master antioxidant	Improves activation of 5 Vit D genes	Daily?

Also    Vitamin C 55

Iodine 32

Iron 68

Vitamin B12 59

Selenium 25

Folate 31

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 Download the PDF from VitaminDWiki

Vitamin D plays a role not only in the proper functioning of the skeletal system and the calcium-phosphate equilibrium, but also in the immune system, the cardiovascular system and the growth and division of cells. Although numerous studies have reported on the analysis of vitamin D status in various groups of patients, the clinical significance of measurements of vitamin D forms and metabolites remains ambiguous. This article reviews the reports analyzing the status of vitamin D in various chronic states. Particular attention is given to factors affecting measurement of vitamin D forms and metabolites. Relevant papers published during recent years were identified by an extensive PubMed search using appropriate keywords. Measurement of vitamin D status proved to be a useful tool in diagnosis and progression of metabolic syndrome, neurological disorders and cancer. High performance liquid chromatography coupled with tandem mass spectrometry has become the preferred method for analyzing the various forms and metabolites of vitamin D in biological fluids. Factors influencing vitamin D concentration, including socio-demographic and biochemical factors as well as the genetic polymorphism of the vitamin D receptor, along with vitamin D transporters and enzymes participating in vitamin D metabolism should be considered as potential confounders of the interpretation of plasma total 25(OH)D concentrations.