Loading...
 
Translate Register Log In Login with facebookLogin and Register

Resveratol helps vitamin D bind to cells – Dec 2014

Resveratrol Potentiates Vitamin D and Nuclear Receptor Signaling†

Journal of Cellular Biochemistry, DOI: 10.1002/jcb.25070
Angelika Dampf-Stone1,‡, Shane Batie1,2,‡, Marya Sabir1,‡, Elizabeth T. Jacobs3,4, Jamie H. Lee1, G. Kerr Whitfield2, Mark R. Haussler2 and Peter W. Jurutka1,2, pjurutka at asu.edu
1 School of Mathematical and Natural Sciences, Arizona State University, Glendale, AZ 85306
2 Department of Basic Medical Sciences, The University of Arizona, College of Medicine, Phoenix, AZ, 85004
3 University of Arizona Cancer Center, Tucson AZ, 85724
4 Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, AZ, 85724

The 1,25-dihydroxyvitamin D3 (1,25D) hormone is derived from vitamin D generated in skin or obtained from the diet, and binds to and activates the vitamin D receptor (VDR) in target tissues including kidney, colon/small intestine, and bone/muscle.
We tested resveratrol for its ability to modulate VDR signaling, using vitamin D responsive element (VDRE) and mammalian two-hybrid (M2H) transcriptional system technology. Via VDRE-based assays in kidney, colon and myoblast cells, VDR-mediated transcription was activated by resveratrol, and a cooperative effect on transactivation was observed with resveratrol plus 1,25D. The M2H assay revealed a modest, resveratrol-induced dimerization of VDR with its retinoid X receptor (RXR) heteropartner.
Cells treated with both resveratrol and 1,25D displayed synergistic stimulation of VDR-RXR heterodimerization, while resveratrol antagonized rexinoid-mediated RXR-RXR homodimerization. Increased transactivation in response to resveratrol was also observed with a subset of other nuclear receptors and their respective cognate responsive elements.
Evaluation of wild-type versus a ligand-binding domain mutant VDR revealed that hormone-responsiveness to 1,25D was severely depressed, while the response to resveratrol was only moderately attenuated.

Moreover, radiolabeled 1,25D-displacement assays demonstrated an increase in VDR-bound 1,25D in the presence of resveratrol.
Thus, resveratrol may affect VDR and other nuclear receptors indirectly, likely via the ability of resveratrol to:

  • 1) potentiate 1,25D binding to VDR,
  • 2) activate RXR, and/or 3) stimulate SIRT1, an enzyme known to deacetylate nuclear receptors.

The results of this study elucidate a possible pathway for crosstalk between two nutritionally derived lipids, vitamin D and resveratrol, both of which converge on VDR signaling.
This article is protected by copyright. All rights reserved


See also VitaminDWiki

VitaminDwiki updated the following chart due to this and previous Resveratrol studies
Reductions in Vitamin D is.gd/VitDReductions


See also web

Attached files

ID Name Comment Uploaded Size Downloads
11065 Resveratrol supplementation, where are we now.pdf admin 19 Dec, 2018 00:02 507.45 Kb 78
See any problem with this page? Report it (FINALLY WORKS)