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Fecal transplants increased Vitamin D levels (germ-free mice) – March 2018

The Gut Microbiota Regulates Endocrine Vitamin D Metabolism through Fibroblast Growth Factor 23

Front. Immunol., 02 March 2018 | https://doi.org/10.3389/fimmu.2018.00408

VitaminDWiki

Started with germ-free mice
Chow included 200 IU of vitamin D 3 days a week (= average of 86 IU daily) for 2 or 4 weeks
Fecal transplants significantly increased the active Vitamin D levels in blood


Many studies have found profound interactions between gut bacteria and Vitamin D

Antibiotics impares the gut bacteria - especially when given before 2 years of age

Antibiotics ==> Obesity?

 Download the PDF from VitaminDWiki
Big increase in just 2 weeks. Wonder how much it would increase in 2 months
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Activated Vitamin D levels increased 2.5X after tranplant
and by 5X if transplant AND 4 weeks of Vitamin D

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Stephanie A. Bora1,2, Mary J. Kennett1, Philip B. Smith2,3, Andrew D. Patterson1,2,3 and Margherita T. Cantorna1,2*
1 Dept. of Veterinary and Biomedical Sciences, The Penn. State University, University Park, PA, United States
2 The Huck Institutes of Life Sciences, The Penn. State University, University Park, PA, United States
3 Eberly College of Science, The Penn. State University, University Park, PA, United States

To determine the effect of the microbiota on vitamin D metabolism, serum 25-hydroxyvitamin D(25D), 24,25-dihydroxyvitamin D (24,25D), and 1,25-dihydroxyvitamin D (1,25D) were measured in germ-free (GF) mice before and after conventionalization (CN). GF mice had low levels of 25D, 24,25D, and 1,25D and were hypocalcemic. CN of the GF mice with microbiota, for 2 weeks recovered 25D, 24,25D, and 1,25D levels.
Females had more 25D and 24,25D than males both as GF mice and after CN.
Introducing a limited number of commensals (eight commensals) increased 25D and 24,25D to the same extent as CN. Monocolonization with the enteric pathogen Citrobacter rodentium increased 25D and 24,25D, but the values only increased after 4 weeks of C. rodentium colonization when inflammation resolved. Fibroblast growth factor (FGF) 23 was extremely high in GF mice. CN resulted in an increase in TNF-α expression in the colon 2 days after CN that coincided with a reduction in FGF23 by 3 days that eventually normalized 25D, 24,25D, 1,25D at 1-week post-CN and reinstated calcium homeostasis. Neutralization of FGF23 in GF mice raised 1,25D, without CN, demonstrating that the high FGF23 levels were responsible for the low calcium and 1,25D in GF mice. The microbiota induce inflammation in the GF mice that inhibits FGF23 to eventually reinstate homeostasis that includes increased 25D, 24,25D, and 1,25D levels. The microbiota through FGF23 regulates vitamin D metabolism.

Created by admin. Last Modification: Saturday June 30, 2018 00:20:01 UTC by admin. (Version 10)

Attached files

ID Name Comment Uploaded Size Downloads
10051 Fecal2.jpg admin 29 Jun, 2018 18:07 10.91 Kb 145
10049 Fecal1.jpg admin 29 Jun, 2018 17:43 9.18 Kb 15
10048 Gut Microbiota Regulates Vitamin d.pdf PDF 2018 admin 29 Jun, 2018 17:14 1.88 Mb 3
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