Osteosarcoma is a type of malignant bone tumor that mainly affects children and adolescents.
vitamin D prevented osteosarcoma cells from replicating as quickly and promoted the growth of normal bone cells.
This study looked at osteosarcoma cells removed from the human body
Press Release by the University
2.5 minute Video by the author
Full article is attached as a PDF at bottom of this page
VitaminDWiki – Cancer - Other category has 66 items along with related searches
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- Association of vitamin D receptor gene polymorphisms with osteosarcoma risk and prognosis - 2019 PDF
- A retrospective observational study on cases of osteosarcomas treated with a multitherapy: The rationale and effectiveness - Sept 2022
- "The multitherapy consisted of somatostatin and analogous (octreotide) all-trans-retinoic acid (ATRA), β-Carotene, axerophthol dissolved in vitamin E, vitamin D, vitamin C, melatonin (MLT), proteoglycans, glycosaminoglycans, hydroxyurea, and sodium butyrate. "
Vitamin D Represses the Aggressive Potential of Osteosarcoma - 2021
Endocrine, Metabolic & Immune Disorders-Drug Targets, Volume 21, #7, 2021, pp. 1312-1318(7)
https://doi.org/10.2174/1871530320666200821155756 publisher wants $65 for the PDF
Tahbazlahafi, Behnoosh; Paknejad, Malihe; Khaghani, Shahnaz; Sadegh-Nejadi, Sahar; Khalili, Ehsan
Background: Osteosarcoma (OS) is the basic bone neoplasm with lower survival and poor prognosis. It is distinguished by its offensive nature and metastatic potential. The fundamental death source in OS patients is lung metastasis. In addition, the proliferation and cell migration are thus essential for cancer progression, especially for intrusion and transformation. Several studies have illustrated that 1,25-Dihydroxyvitamin D (1,25(OH)2D) has a critical role in the growth and differentiation of bone. However, knowledge of the outcome of 1,25(OH)2D on the progression and incursion of osteosarcoma cells is minimal.
Objective: The present study aimed to analyze the effect of different concentrations of 1,25(OH)2D on the multiplication, progression, and intrusion of OS cells and verify the effective doses of 1,25(OH)2D that can decrease the intensity of the disease and improving the prognosis in OS patients.
Methods: Saos-2 cells were treated with 1,25(OH)2D (0, 50, 100, and 200 nM) for 48, 72, and 96 hours. Proliferation, invasion, and migration were determined by MTT assay, Transwell assay, and Scratch test, respectively. The levels of c-Myc and FOXO1 proteins were determined by Western blotting.
Results: The proliferation, invasiveness, and migration of Saos-2 cells that were treated with 1,25(OH)2D were significantly decreased compared with untreated cells. Although 1,25(OH)2D notably decreased c-Myc protein levels (after 48 and 72 hours), FOXO1 protein levels have been significantly increased after 48 and 72 hours. 1,25(OH)2D and the vitamin D receptor (VDR) suppress c-Myc function through regulating the c-Myc/MXD1 network and thus, providing a molecular basis of 1,25(OH)2D related to the cancer-preventive actions.
Conclusion: Based on the present results, 1,25(OH)2D by targeting c-Myc and FOXO1 expression displays anti-invasive, anti-migration and anti-proliferative effects on OS cells in vitro. Our findings suggest that effective doses of the 1,25(OH)2D may reduce the aggressive potential of the OS cell line. However, further investigation and clinical trials are needed.
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Web
- Association of vitamin D receptor gene polymorphisms with osteosarcoma risk and prognosis - 2019 PDF
- A retrospective observational study on cases of osteosarcomas treated with a multitherapy: The rationale and effectiveness - Sept 2022
- "The multitherapy consisted of somatostatin and analogous (octreotide) all-trans-retinoic acid (ATRA), β-Carotene, axerophthol dissolved in vitamin E, vitamin D, vitamin C, melatonin (MLT), proteoglycans, glycosaminoglycans, hydroxyurea, and sodium butyrate. "
Vitamin D Represses the Aggressive Potential of Osteosarcoma - 2021
Endocrine, Metabolic & Immune Disorders-Drug Targets, Volume 21, #7, 2021, pp. 1312-1318(7)
https://doi.org/10.2174/1871530320666200821155756 publisher wants $65 for the PDF
Tahbazlahafi, Behnoosh; Paknejad, Malihe; Khaghani, Shahnaz; Sadegh-Nejadi, Sahar; Khalili, Ehsan
Background: Osteosarcoma (OS) is the basic bone neoplasm with lower survival and poor prognosis. It is distinguished by its offensive nature and metastatic potential. The fundamental death source in OS patients is lung metastasis. In addition, the proliferation and cell migration are thus essential for cancer progression, especially for intrusion and transformation. Several studies have illustrated that 1,25-Dihydroxyvitamin D (1,25(OH)2D) has a critical role in the growth and differentiation of bone. However, knowledge of the outcome of 1,25(OH)2D on the progression and incursion of osteosarcoma cells is minimal.
Objective: The present study aimed to analyze the effect of different concentrations of 1,25(OH)2D on the multiplication, progression, and intrusion of OS cells and verify the effective doses of 1,25(OH)2D that can decrease the intensity of the disease and improving the prognosis in OS patients.
Methods: Saos-2 cells were treated with 1,25(OH)2D (0, 50, 100, and 200 nM) for 48, 72, and 96 hours. Proliferation, invasion, and migration were determined by MTT assay, Transwell assay, and Scratch test, respectively. The levels of c-Myc and FOXO1 proteins were determined by Western blotting.
Results: The proliferation, invasiveness, and migration of Saos-2 cells that were treated with 1,25(OH)2D were significantly decreased compared with untreated cells. Although 1,25(OH)2D notably decreased c-Myc protein levels (after 48 and 72 hours), FOXO1 protein levels have been significantly increased after 48 and 72 hours. 1,25(OH)2D and the vitamin D receptor (VDR) suppress c-Myc function through regulating the c-Myc/MXD1 network and thus, providing a molecular basis of 1,25(OH)2D related to the cancer-preventive actions.
Conclusion: Based on the present results, 1,25(OH)2D by targeting c-Myc and FOXO1 expression displays anti-invasive, anti-migration and anti-proliferative effects on OS cells in vitro. Our findings suggest that effective doses of the 1,25(OH)2D may reduce the aggressive potential of the OS cell line. However, further investigation and clinical trials are needed.
| 6599 visitors, last modified 01 Oct, 2022, |