Obesity is associated with 1 to 5 poor vitamin D genes (childhood obesity in the case) – July 2024

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Decreased vitamin D bio-availability with altered DNA methylation of its metabolism genes in association with the metabolic disorders among the school-aged children with degree I, II, and III obesity

J. of Nutritional Biochemistry, Vol. 129, July 2024, https://doi.org/10.1016/j.jnutbio.2024.109627 PDF behind paywall
Xueyi Jiang a †, Lulu Xia b †, Tiantian Tang a, Xiuqin Fan a, Rui Wang a, Meichen Wang b, Wenli Yang b, Jie Yan b, Kemin Qi a, Ping Li a

Obesity is strongly associated with disturbances of vitamin D (VD) metabolites in the animal models. However, the related epidemiological evidence is still controversial, especially the different degrees of obesity children. Hence, in this present representative case-control study, 106 obesity school-age children aged 7–12 years were included and divided into different subgroups as degree

• I (the age- and sex-specific BMI≥95th percentile, n=45),
• II (BMI ≥120% percentile, n=34) and
• III (BMI ≥140% percentile, n=27)

obesity groups across the ranges of body mass index (BMI). While the age- and sex-matched subjects without obesity were as the control group. Notably, it was significantly different of body composition, anthropological and clinical characteristics among the above four subgroups with the dose-response relationships (P<.05). Moreover, comparing with the control group, the serum VD concentrations were higher, VD metabolites like 25(OH)D, 25(OH)D3 and 1,25(OH)2D, and related hydroxylases as CYP27A1, CYP2R1 and CYP27B1 were lower in the degree I, II, and III obesity subgroups (P<.05), which were more disorder with the anthropological and clinical characteristics as the obesity was worsen in a BMI-independent manner (P<.05).
However, there was a significant increase of CYP27B1 in the degree III obesity group than those in the degree I and II obesity subgroups.
Furthermore, the methylation patterns on the genome-wide (Methylation/Hydroxymethylation) and VD metabolism genes (CYP27A1, CYP2R1 and CYP27B1) were negatively correlated with the worse obesity and their related expressions (P<.05).
In summary, these results indicated that obesity could affect the homeostasis of VD metabolism related genes such as CYP27A1, CYP2R1, CYP27B1 and etc through abnormal DNA methylation, resulting in the disorders of VD related metabolites to decrease VD bio-availability with the BMI-independent manner. In turn, the lower levels of VD metabolites would affect the liver function to exacerbate the progression of obesity, as the Degree II and III obesity subgroups.

Introduction
Obesity in the children and adolescents aged 5–19 year represents a global health issue with an ever-increasing prevalence, as WHO has estimates that it has nearly tripled since 1975–2018 [1,2]. In China, the children obesity increases rapidly [3]. As we all known, obesity is associated with a strong predisposition towards metabolic diseases and even cancer among the childhood and even adulthood [3,4]. Thus, early prevention of children obesity is of great significance for minimizing the adverse consequences through the entire life cycle. Notably, the main characteristics of obesity is the abnormal expansion of adipose depots, which can be driven by the imbalance of hypertrophy and hyperplasia [5]. Generally, the hyperplasia of adipocytes plays an important role on the early stages of obesity to be considered healthy and adaptive by maintaining the proper vascularization, increasing many metabolism modulatory adipokines and so on [5,6]. However, with the further progresses of obesity, the hypertrophy of adipocytes with massively expanded expansion posits the most important outcomes, which is associated with the limitations of oxygen diffusion and angiogenesis to elevate the lipolysis, increase the secretion of inflammatory cytokines and reduce the adipokines to persistently contribute the occurrence of metabolic diseases [7,8]. Here, it is becoming more crucial to explore the different grades of childhood obesity as the degree I, II, and III obesity clearly for their clinical interventions using the more detailed researches.

As previously described, the causation of childhood obesity are complex and multifaceted, presenting the clinicians with myriad challenges. Moreover, a key contributor of micronutrients is an significant element to prevent the later chronic diseases of children obesity by arising the energy metabolism, adipogenesis process, inflammation status and etc. in the adipocytes. Hence, it is necessary to study the relationships between the micronutrients intake and obesity [9,10], in which vitamin D (VD) deficiency has been reported commonly among the Chinese children and adolescents [11,12]. Recently, many researches have confirmed that both VD insufficient intake and its decreased bio-availability have been associated with features of metabolic syndrome using the animal experiments by affecting the insulin resistance, endocannabinoid tone, glucose and lipid metabolism, gut dysbiosis, chronic inflammation, differentiation of adipocytes and etc. [13], [14], [15], However, it is still unknown about the explanations for the increasing risks of VD deficiency in the development of obesity. Of note, more than 50% patients of metabolic diseases have low circulating levels of total and free 25-hydroxyvitamin D [25(OH)D], including the 25(OH)D2 and 25(OH)D3, and 1,25 dihydroxyvitamin D [1,25(OH)2D][16].
However, different cohorts have put forward the inconsistent effectiveness on the cutoff points for VD deficiency on the progress of obesity, incompatible VD metabolites (25(OH)D, 1,25(OH)2D) due to no consensus of cause-effect relationships, different ages and grades of obesity and so on [17], [18], [19]. Meanwhile, there is few researches on the dose-responses and causal relationships between the concentrations of VD metabolites and different grades of obesity. To go further in determining the impacts of VD status on the progression of obesity and its related metabolic disorders, we implemented a longitudinal study of school-aged children aged 7–12 years with degree I, II, and III obesity to explore the VD bio-availability (total VD, 25(OH)D and 1,25(OH)2D) and its correlations with the clinical characteristics, which could provide the appropriate basis for personalizing the VD interventions among the school-aged children with different grades of obesity.

Notably, there are two hydroxylation steps on catalyzing the VD into their biologically active metabolites (25(OH)D and 1,25(OH)2D) [17], [18], [19].
On the first step, the endogenous or dietary VD is enzymatically hydroxylated to 25(OH)D, which is catalyzed by CYP2R1 (vitamin D 25-hydroxylase) and CYP27A1 (Cholesterol 27α hydroxylase) as the key enzymes of 25 hydroxylation.
On the second step, the VDBP-bound 25(OH)D is then transported to the kidneys and various other organs to be metabolized into 1,25(OH)2D by CYP27B1 (25-hydroxyvitamin D-1-α hydroxylase). Then 1,25(OH)2D is attached to the vitamin D receptor (VDR), as a transcription factor in the steroid hormone receptor super-family in the cytoplasm, which is transported into the nucleus and acted as the ligand-dependent transcription factor to regulate the target genes to exert their corresponding biological functions [18].
Therefore, the disorders of these VD metabolic pathway genes (CYP27A1, CYP2R1, CYP27B1, VDR and VDBP) in the two hydroxylation steps could be related with the decreases of 25(OH)D and/or 1,25(OH)2D during the occurrence of many diseases such as Rheumatoid Arthritis, tuberculosis, type 2 diabetes, cancer and so on [20], [21], [22], [23]. Moreover, it suggests that DNA methylation is one of the most common epigenetic mechanisms for the changes in gene expressions. High methylation levels in the promoter region of VD metabolic pathway genes may cause gene silencing to affect vitamin D response variation [20], [21], [22], [23]. However, the relationships between the altered DNA methylation on these VD metabolic pathway genes and progress of obesity still need to be further verified. Therefore, the aim of this study was to test the hypothesis that obesity could disrupt the VD homeostasis via modulating the related gene expressions by DNA methylation to conversely aggravate the metabolic disorders among the school-aged children under the degree I, II, and III obesity.

Section snippets
Selection of the subjects and subgroups
This representative Case-control study, including 106 obesity children who were adjusted by the body mass index (BMI) and 45 sex- and age-matched subjects without obesity as the control group, was carried out at Beijing Children's Hospital from July 2018 to April 2021 under the strict inclusion and exclusion criteria. Specifically, the criteria for participation were as follows: The inclusion criteria consisted that the subjects were 7–12 year healthy school-age children without obvious….

Basic characteristics of the subjects in different degrees of obese children and controls
The basic characteristics of 151 participants (106 obese children and 45 healthy children) by the category of BMI were shown in Table 1. Overall, the number of no-obese children was 45 as the control group. While the 45 (42.45%), 34 (32.08%) and 27 (25.47%) obese children were respectively categorized as the degree I, II, and III obesity subgroups. Comparing with the control group (weight: 55.02±2.34 kg, BMI: 22.99±0.76 kg/m2, waist hip ratio: 0.85±0.012, WAZ: 3.49±0.51 and BMI Z: 0.89±0.024),….

Discussion
Children obesity and its related disease complications reduce the life quality and expectancy and increase health care costs [27]. Many studies also have suggested that obesity always increases the risk of diabetes, liver injury cardiometabolic complications and even cancer with respect to the obesity-related metabolic dysfunctions [27]. Thus, the identification of individual metabolites and discrete pathways able to distinguish the different degrees of obesity with more refined studies help to ….

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17 Items in both of the categories of Vitamin D Receptor AND Obesity

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VitaminDWiki - Vitamin D Receptor activation can be increased in 16 ways

Resveratrol,  Omega-3,  Magnesium,  Zinc,   Quercetin,   non-daily Vit D,  Curcumin,   Berberine,  intense exercise, Butyrate   Sulforaphane   Ginger,   Essential oils, etc  Note: The founder of VitaminDWiki uses 10 of the 16 known VDR activators

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4+ VitaminDWiki Obesity pages have CYP27A1, CYP2R1, CYP27B1, OR BINDING in the title

This list is automatically updated

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VitaminDWiki – Genetics category contains

346 articles in the Genetics category

see also

• Vitamin D Receptor has 537 items
• Vitamin D Binding Protein = GC has 178 items
• CYP27B1 has 64 items
• CYP24A1 in title of 39+ items
• CYP2R1 25+ items
• Calcidiol has 49 items
• Calcitriol has 64 items
• Topical Vitamin D
• Nanoemulsion Vitamin D may be a substantially better form
• 1289 genes changed with higher doses of Vitamin D - RCT Dec 2019
• CYP3A4 (7 as of Dec 2022)
• Getting Vitamin D into your blood and cells
• 

Vitamin D blood test misses a lot

• Vitamin D from coming from tissues (vs blood) was speculated to be 50% in 2014, and by 2017 was speculated to be 90%
• Note: Good blood test results (> 40 ng) does not mean that a good amount of Vitamin D actually gets to cells
• A Vitamin D test in cells rather than blood was feasible (2017 personal communication)   Commercially available 2019
  • However, test results would vary in each tissue due to multiple genes
• Good clues that Vitamin D is being restricted from getting to the cells
  1) A vitamin D-related health problem runs in the family
      especially if it is one of 51+ diseases related to Vitamin D Receptor
  2) Slightly increasing Vitamin D shows benefits (even if conventional Vitamin D test shows an increase)
  3) DNA and VDR tests - 100 to 200 dollars $100 to $250
  4) PTH bottoms out ( shows that parathyroid cells are getting Vitamin d)
     Genes are good, have enough Magnesium, etc.
  5) Back Pain
     probably want at least 2 clues before taking adding vitamin D, Omega-3, Magnesium, Resveratrol, etc
  • The founder of VitaminDWiki took action with clues #3&5