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Autoimmune system, Dendric cells and active Vitamin D (technical) March 2019

1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD31

Front. Immunol., 28 March 2019 | https://doi.org/10.3389/fimmu.2019.00600
Louise Saul1†, Iris Mair1†, Alasdair Ivens2, Pamela Brown3, Kay Samuel4, John D. M. Campbell4, Daniel Y. Soong5, Nadine Kamenjarin1 and Richard J. Mellanby1,6*

VitaminDWiki

Pathogen Strategies to Evade Innate Immune Response: A Signaling Point of View

June 2012, free PDF, DOI: 10.5772/37771.
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Regulatory dendritic cells: there is more than just immune activation

Sept 2012, free PDF, https://doi.org/10.3389/fimmu.2012.00274
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Study described - based on a press release which highlighted MS, while MS was only mentioned once in the study



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Dendritic cells (DC) are specialized sentinel cells that bridge the innate and adaptive immune response and play a crucial role in shaping the adaptive immune response. Vitamin D, a known epidemiological risk factor for the development of several autoimmune diseases, influences the development of dendritic cells. Consequently, vitamin D metabolites are frequently used in protocols to develop therapeutic dendritic cell therapies for autoimmune diseases. However, the mechanisms by which vitamin D modulates DC function remain poorly understood. We investigated the effects of vitamin D on murine CD11c+ bone marrow derived DC (BMDC) function by analyzing global gene expression in CD11c+ BMDC generated in the presence (VitD-CD11c+BMDC) or absence (Veh-CD11c+BMDC) of the active vitamin D metabolite, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). Seven genes were significantly increased in expression in both immature and LPS-matured VitD-CD11c+BMDC, one of which was CD31, a member of the immunoglobulin superfamily. Gene knockdown of CD31 enhanced the ability of VitD-CD11c+BMDC to prime naïve CD4+ T cells in vitro; conversely, increased expression of CD31 on vehicle treated CD11c+BMDC restrained their T cell priming abilities. Time-lapse imaging of BMDC and CD4+ T cells during in vitro priming revealed that CD31 reduced the BMDC–T cell interaction time. Finally, we confirmed a similar effect of 1,25(OH)2D3 on human CD34+ cell-derived CD11c+DC, whereby DC generated in the presence of 1,25(OH)2D3 had increased CD31 expression. In summary, we show that both mouse and human DC generated in the presence of 1,25(OH)2D3 upregulate CD31 expression, resulting in a reduced ability to prime CD4+ T cells by impairing a stable cell-cell contact.

Created by admin. Last Modification: Tuesday April 16, 2019 14:42:36 GMT-0000 by admin. (Version 5)

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11776 DC stim and reg.jpg admin 16 Apr, 2019 09:33 55.45 Kb 22
11775 DC link.jpg admin 16 Apr, 2019 09:32 39.87 Kb 21
11774 fimmu-10-00600.pdf PDF 2019 admin 16 Apr, 2019 09:10 3.26 Mb 2
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