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54 genes significantly affected by Vitamin D (50,000 IU weekly for 12 weeks) – May 2021

Immunomodulatory Effects of Vitamin D Supplementation in a Deficient Population

Int. J. Mol. Sci. 2021, 22(9), 5041; https://doi.org/10.3390/ijms22095041
Mathieu Garand 1,*OrcID,Mohammed Toufiq 1OrcID,Parul Singh 1OrcID,Susie Shih Yin Huang 1,Sara Tomei 1OrcID,Rebecca Mathew 1,Valentina Mattei 1OrcID,Mariam Al Wakeel 2,Elham Sharif 2,*,†OrcID andSouhaila Al Khodor 1,*,†OrcID
1 Research Department, Sidra Medicine, Doha 26999, Qatar
2 Dept. of Biomedical Sciences, College of Health Sciences, Qatar Univ., Doha 26999, Qatar
Note: 9.6% used vitamin D2 - which is known to have a very poor response to non-daily dosing
Note: 34% had high BMI - so would would have needed more than 50,000 IU



Note: The study on this page started with women with very low levels of vitamin D
On average they achieved 34 ng in 12 weeks
Starting with more tipical levels of vitamin most would have achieved at least 40 ng during the trial
Starting with a loading dose would have resulted in most getting to 40 ng in less two weeks

 Download the PDF from VitaminDWiki

In addition to its canonical functions, vitamin D has been proposed to be an important mediator of the immune system. Despite ample sunshine, vitamin D deficiency is prevalent (>80%) in the Middle East, resulting in a high rate of supplementation. However, the underlying molecular mechanisms of the specific regimen prescribed and the potential factors affecting an individual’s response to vitamin D supplementation are not well characterized. Our objective is to describe the changes in the blood transcriptome and explore the potential mechanisms associated with vitamin D3 supplementation in one hundred vitamin D-deficient women who were given a weekly oral dose (50,000 IU) of vitamin D3 for three months. A high-throughput targeted PCR, composed of 264 genes representing the important blood transcriptomic fingerprints of health and disease states, was performed on pre and post-supplementation blood samples to profile the molecular response to vitamin D3.
We identified 54 differentially expressed genes that were strongly modulated by vitamin D3 supplementation. Network analyses showed significant changes in the immune-related pathways such as TLR4/CD14 and IFN receptors, and catabolic processes related to NF-kB, which were subsequently confirmed by gene ontology enrichment analyses. We proposed a model for vitamin D3 response based on the expression changes of molecules involved in the receptor-mediated intra-cellular signaling pathways and the ensuing predicted effects on cytokine production. Overall, vitamin D3 has a strong effect on the immune system, G-coupled protein receptor signaling, and the ubiquitin system. We highlighted the major molecular changes and biological processes induced by vitamin D3, which will help to further investigate the effectiveness of vitamin D3 supplementation among individuals in the Middle East as well as other regions.

Clipped from PDF__

"...observed a downregulation trend that was consistently more pronounced in the NR group

Wonder what implies

Created by admin. Last Modification: Wednesday January 4, 2023 02:11:12 GMT-0000 by admin. (Version 12)

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15574 genes 50K.jpg admin 10 May, 2021 163.16 Kb 752
15573 50K weekly.jpg admin 10 May, 2021 97.63 Kb 717
15572 Immunomodulatory Effects of Vitamin D_compressed.pdf admin 10 May, 2021 912.05 Kb 482