Breast cancer – reduced proliferation with Vitamin K2 in lab – May 2015

Real Time Cell Analysis of the inhibitory effect of Vitamin K2 on adhesion and proliferation of breast cancer cells

Nutrition Research; Available online 30 May 2015
Received 5 November 2014, Revised 12 May 2015, Accepted 27 May 2015, Available online 30 May 2015
Maeve Kielya, b, c; Spencer J. Hodgins d; B. Anne Merrigan e; Shona Tormey d, e,
Patrick A. Kiely a, b, c; Eibhlís M. O’Connor a
a Department of Life Sciences, University of Limerick
b Materials and Surface Science Institute, University of Limerick
c Stokes Institute, University of Limerick
d Graduate Entry Medical School, University of Limerick
e Department of Surgery, University Hospital Limerick

Breast cancer is the most prevalent cancer type worldwide [1]. Continued efforts to improve treatment strategies for patients with breast cancer will be instrumental in reducing the death rates associated with this disease. In particular, the triple negative breast cancer (TNBC) subtype of breast cancer has no targeted therapy available so it is essential to continue to work on any potential therapies. Vitamin K (VK), is known for its essential role in the clotting cascade.

The anti-tumour properties of VK derivatives have been reported in both hepatocellular carcinoma and glioblastoma.

Our hypothesis was that menaquinone-4, the most common form of VK2 is an effective anti-cancer agent against breast cancer cell types. In this study we used a novel impedance-based live cell monitoring platform (xCELLigenceTM) to determine the effects of VK derivatives on the TNBC cell line, MDA-MB-231 and the HER2+ breast cancer cell line, MDA-MB-453.

Cells were treated with varying concentrations of menaquinone-4 (VK2) previously reported to have an anti-proliferative effect on human glioblastoma cells. After initial testing, these concentrations were adjusted to

• 100μM,
• 125μM and
• 150μM.

A significant dose-dependent, growth inhibitory effect was found when cells were treated at these concentrations. These effects were seen in both adhesion and proliferation phases and a dramatic reduction in cell growth. Additional analysis of MDA-MB-231 cells treated with VK2 (100μM) in combination with a low glucose nutrient media showed a further decrease in adhesion and viability. This is the first study of its kind showing the real-time effects of VK derivatives on breast cancer cells and suggests that dietary factors may be an important consideration for patients.

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I have no idea how much Vitamin K2 this would be: 100 micrograms, 1,000 micrograms, etc.
How much reduction was found? 1%. 10%. 50%
Vitamin K2 is good for many things. This is the first time I can recall seeing that Vitamin K2 helps reduce Cancer

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