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Smoke Inflammation reduced by vitamin D (COPD in lab) – Sept 2019

Vitamin D Modulates the Response of Bronchial Epithelial Cells Exposed to Cigarette Smoke Extract

Nutrients 2019, 11(9), 2138; https://doi.org/10.3390/nu11092138


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The higher the Vitamin D concentration, the lower the inflamation

Carolien Mathyssen 1OrcID,Jef Serré 1OrcID,Annelore Sacreas 1,Stephanie Everaerts 1,Karen Maes 1,Stijn Verleden 1,Lieve Verlinden 2,Annemieke Verstuyf 2,Charles Pilette 3,Ghislaine Gayan-Ramirez 1,Bart Vanaudenaerde 1 andWim Janssens 1,
1 Lab of Respiratory Diseases, CHROMETA, KU Leuven, 3000 Leuven, Belgium
2 Clinical and Experimental Endocrinology, CHROMETA, KU Leuven, 3000 Leuven, Belgium
3 Institute of Experimental & Clinical Research—Pole of Pneumology, ENT and Dermatology, Université Catholique de Louvain (UCL), 1200 Brussels, Belgium

In chronic obstructive pulmonary disease (COPD), the bronchial epithelium is the first immune barrier that is triggered by cigarette smoke. Although vitamin D (vitD) has proven anti-inflammatory and antimicrobial effects in alveolar macrophages, little is known about the direct role of vitD on cigarette smoke-exposed bronchial epithelial cells. We examined the effects of vitD on a human bronchial epithelial cell line (16HBE) and on air–liquid culture of primary bronchial epithelial cells (PBEC) of COPD patients and controls exposed for 24 h to cigarette smoke extract (CSE). VitD decreased CSE-induced IL-8 secretion by 16HBE cells, but not by PBEC. VitD significantly increased the expression of the antimicrobial peptide cathelicidin in 16HBE and PBEC of both COPD subjects and controls. VitD did not affect epithelial to mesenchymal transition or epithelial MMP-9 expression and was not able to restore impaired wound healing by CSE in 16HBE cells. VitD increased the expression of its own catabolic enzyme CYP24A1 thereby maintaining its negative feedback.

In conclusion, vitD supplementation may potentially reduce infectious exacerbations in COPD by the upregulation of cathelicidin in the bronchial epithelium.

Created by admin. Last Modification: Friday September 6, 2019 18:31:01 GMT-0000 by admin. (Version 5)

Attached files

ID Name Comment Uploaded Size Downloads
12591 Higher D less IL-8.jpg admin 06 Sep, 2019 24.21 Kb 632
12590 COPD smoke lab.pdf admin 06 Sep, 2019 410.66 Kb 674