Circadian Rhythms may be influenced by vitamin D
A Role for 1α,25-Dihydroxyvitamin D3 in the Expression of Circadian Genes.
J Biol Rhythms. 2014 Sep 17. pii: 0748730414549239. [Epub ahead of print]
Gutierrez-Monreal MA1, Cuevas-Diaz Duran R2, Moreno-Cuevas JE2, Scott SP3.
Author information
The active form of vitamin D, 1α,25-(OH)2D3, has been associated with metabolism control, cell growth, differentiation, antiproliferation, apoptosis, and adaptive/innate immune responses, besides its functions in the integrity of bone and calcium homeostasis. The circadian rhythm regulates a variety of biological processes, many of them related to the functions associated with 1α,25-(OH)2D3. In the present study, we determine whether 1α,25-(OH)2D3 alters the expression of circadian genes in adipose-derived stem cells (ADSCs). The effect of 1α,25-(OH)2D3 on the expression of circadian genes BMAL1 and PER2 was measured by qPCR, over a 60-h period every 4 h, in serum shocked ADSCs, serum shocked ADSCs supplemented with 1α,25-(OH)2D3, and ADSCs under the presence of only 1α,25-(OH)2D3. The results showed that 1α,25-(OH)2D3 was able to synchronize circadian clock gene expression in ADSCs. The expression of circadian genes BMAL1 and PER2 in ADSCs that contained only 1α,25-(OH)2D3 has a profile similar to that found in the ADSCs synchronized by a serum shock. The results suggest an important role of 1α,25-(OH)2D3 in the regulation of the molecular clock.
© 2014 The Author(s).
KEYWORDS: BMAL1; PER2; adipose-derived stem cells; serum shock; vitamin D
PMID: 25231949