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Vitamin D parameters vary about 4% daily – Sept 2017

Diurnal rhythms of vitamin D binding protein and total and free vitamin D metabolites.

J Steroid Biochem Mol Biol. 2017 Sep;172:130-135. doi: 10.1016/j.jsbmb.2017.07.015. Epub 2017 Jul 19.
Jones KS1, Redmond J2, Fulford AJ3, Jarjou L4, Zhou B5, Prentice A6, Schoenmakers I2.

VitaminDWiki summary

Detailed blood measurements from 3 countries (first of the day was after fasting)
Numbers are CCV% = coefficient of cyclic variation

Daily variation in parameter (CCV%)

MeasurementBritain Gambia China
1,25(OH)2D 7.7 3.43.8
Binding Protein4.53.14.8
Free 25(OH)D
Free 1,25(OH)2D

British = orange.    Gambian = blue   Chinese = green





Binding Protein




Free 25(OH)D


Free 1,25(OH)2D


 Download the PDF from VitaminDWiki

Vitamin D binding protein (DBP) concentration is known to influence the availability and bioactivity of vitamin D metabolites but its diurnal rhythm (DR), its inter-relationships with the DRs of vitamin D metabolites and its influence on free vitamin D metabolite concentrations are not well described. The DRs of plasma total 25(OH)D, total 1,25(OH)2D, DBP, albumin and calculated free 25(OH)D and free 1,25(OH)2D were measured in men and women aged 60-75 years and resident in the UK (n 30), Gambia (n 31) and China (n 30) with differences in lifestyle, dietary intake and vitamin D status. Blood samples were collected every 4h for 24h and DRs statistically analysed with Fourier regression.

Gambians had significantly higher plasma concentrations of vitamin D metabolites and lower albumin concentration compared to the British and Chinese.
Significant DRs were observed for all analytes and calculated free vitamin D metabolites (P<0.01). The pattern of DRs was similar between countries. The magnitude of the DRs of free 1,25(OH)2D was attenuated compared to that of total 1,25(OH)2D whereas it was not different between total and free 25(OH)D. Relationships between the DRs were generally weak.

There was no phase shift between 1,25(OH)2D and DBP with the strongest cross correlation at 0h time lag (r=0.15, P=<0.001).

In comparison, 25(OH)D correlated less well with DBP (1h time lag, r=0.07, P=0.12). These data demonstrate a relationship between the DRs of 1,25(OH)2D and DBP, possibly to maintain free 1,25(OH)2D concentrations.

In contrast, the DRs of total and free 25(OH)D appeared to be less influenced by DBP, suggesting that DBP has comparatively less effect on 25(OH)D concentration and 25(OH)D availability. This work highlights the importance of standardisation in timing of sample collection particularly for the assessment of plasma protein concentrations.

PMID: 28732681 PMCID: PMC5571031 DOI: 10.1016/j.jsbmb.2017.07.015

Created by admin. Last Modification: Monday December 25, 2017 03:01:22 UTC by admin. (Version 11)

Attached files

ID Name Comment Uploaded Size Downloads
9021 DBP.jpg admin 25 Dec, 2017 02:45 19.67 Kb 47
9019 Albumin.jpg admin 25 Dec, 2017 02:37 22.69 Kb 46
9018 Free 1,25.jpg admin 25 Dec, 2017 02:36 21.74 Kb 45
9017 Free 25.jpg admin 25 Dec, 2017 02:36 20.87 Kb 46
9016 1.25.jpg admin 25 Dec, 2017 02:36 22.86 Kb 46
9015 25.jpg admin 25 Dec, 2017 02:35 23.66 Kb 48
9014 1712-Vit D diurnal variations Jones.pdf PDF 2017 admin 25 Dec, 2017 02:33 430.17 Kb 41
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