Feasibility of self-sampled dried blood spot and saliva samples sent by mail in a population-based study.
BMC Cancer. 2015 Apr 11;15(1):265. [Epub ahead of print]
Sakhi AK1,2, Bastani NE3, Ellingjord-Dale M4, Gundersen TE5, Blomhoff R6,7, Ursin G8,9,10.
1Norwegian Institute of Public Health, 0456, Oslo, Norway. Amrit.Sakhi at fhi.no.
2Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, 0316, Oslo, Norway. Amrit.Sakhi at fhi.no.
3Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, 0316, Oslo, Norway. nasserb at medisin.uio.no.
4Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, 0316, Oslo, Norway. merete.ellingjord-dale at kreftregisteret.no.
5Vitas AS, Oslo Innovation Park, N-0349, Oslo, Norway. teg at vitas.no.
6Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, 0316, Oslo, Norway. runeb at medisin.uio.no.
7Department of Clinical Service, Division of Cancer Medicine, Surgery and Transplantation, Oslo University Hospital, 0424, Oslo, Norway. runeb at medisin.uio.no.
8Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, 0316, Oslo, Norway. giske.ursin at kreftregisteret.no.
9Cancer Registry of Norway, P.O. Box 5313, 0304, Oslo, Norway. giske.ursin at kreftregisteret.no.
10Department of Preventive Medicine, University of Southern California, 90032-3628, Los Angeles, CA, USA. giske.ursin at kreftregisteret.no.
In large epidemiological studies it is often challenging to obtain biological samples. Self-sampling by study participants using dried blood spots (DBS) technique has been suggested to overcome this challenge. DBS is a type of biosampling where blood samples are obtained by a finger-prick lancet, blotted and dried on filter paper. However, the feasibility and efficacy of collecting DBS samples from study participants in large-scale epidemiological studies is not known. The aim of the present study was to test the feasibility and response rate of collecting self-sampled DBS and saliva samples in a population-based study of women above 50 years of age.
We determined response proportions, number of phone calls to the study center with questions about sampling, and quality of the DBS. We recruited women through a study conducted within the Norwegian Breast Cancer Screening Program. Invitations, instructions and materials were sent to 4,597 women. The data collection took place over a 3 month period in the spring of 2009.
Response proportions for the collection of DBS and saliva samples were 71.0% (3,263) and 70.9% (3,258), respectively. We received 312 phone calls (7% of the 4,597 women) with questions regarding sampling. Of the 3,263 individuals that returned DBS cards, 3,038 (93.1%) had been packaged and shipped according to instructions. A total of 3,032 DBS samples were sufficient for at least one biomarker analysis (i.e. 92.9% of DBS samples received by the laboratory). 2,418 (74.1%) of the DBS cards received by the laboratory were filled with blood according to the instructions (i.e. 10 completely filled spots with up to 7 punches per spot for up to 70 separate analyses). To assess the quality of the samples, we selected and measured two biomarkers (carotenoids and vitamin D). The biomarker levels were consistent with previous reports.
Collecting self-sampled DBS and saliva samples through the postal services provides a low cost, effective and feasible alternative in epidemiological studies.
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Wish that the study incidated the spread of results for measurements taken on the same card
Interesting that they included drying agents - unaware of drying agents in other dried blood spot testing
- Vitamin D Testers coming soon (low cost and easy) - June 2014 much less expensive that dried blood spot
- No vitamin D test needed before supplementing (typically) – April 2014
- Warning – Medicare will pay for just 1 vitamin D test PER LIFETIME (if not related to a few diseases)
- Low cost vitamin D Blood Tests includes several companies offering dried blood spot testing services
- Instant vitamin D Yes-No test now available - home testing, not dried blood spot, perhaps lower cost