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More than 5X increase in activation of scores of genes for women with Vitamin D (20,000 IU weekly) RCT Oct 2017

Changes in the human transcriptome upon vitamin D supplementation.

J Steroid Biochem Mol Biol. 2017 Oct;173:93-99. doi: 10.1016/j.jsbmb.2017.03.016. Epub 2017 Mar 19.
Pasing Y1, Fenton CG2, Jorde R3, Paulssen RH2.

VitaminDWiki Summary

Note: 20,000 IU weekly raised Vitamin D levels to 48 ng average
Genetics category listing contains the following

277 articles in the Genetics category

see also

Vitamin D blood test misses a lot
Blood Test Misses a lot (VDW 3439)

  • Snapshot of the literature by VitaminDWiki as of early 2019
  • Vitamin D from coming from tissues (vs blood) was speculated to be 50% in 2014, and by 2017 was speculated to be 90%
  • Note: Good blood test results (> 40 ng) does not mean that a good amount of Vitamin D actually gets to cells
  • A Vitamin D test in cells rather than blood was feasible (2017 personal communication)
  •    Commercially available 2019
    • However test results would vary in each tissue due to multiple genes
  • Good clues that Vitamin D is being restricted from getting to the cells
    1) A vitamin D-related health problem runs in the family
    2) Slightly increasing Vitamin D show benefits (even if conventional Vitamin D test shows an increase)
    3) Vitamin D Receptor test (<$30) scores are difficult to understand in 2016
    • easier to understand the VDR 23andMe test results analyzed by FoundMyFitness in 2018

    4) Back Pain

Vitamin D is hydroxylated in the liver and kidneys to its active form, which can bind to the vitamin D receptor (VDR). The VDR is present in a wide variety of different cells types and tissues and acts as a transcription factor. Although activation of the VDR is estimated to regulate expression of up to 5% of the human genome, our study is the first analysing gene expression after supplementation in more than 10 subjects. Subjects of a randomized controlled trial (RCT) received either vitamin D3 (n=47) in a weekly dose of 20,000 IU or placebo (n=47) for a period of three to five years. For this study, blood samples for preparation of RNA were drawn from the subjects and mRNA gene expression in blood was determined using microarray analysis. The two study groups were similar regarding gender, age, BMI and duration of supplementation, whereas the mean serum 25-hydroxyvitamin D (25(OH)D) level as expected was significantly higher in the vitamin D group (119 versus 63nmol/L).
When analysing all subjects, nearly no significant differences in gene expression between the two groups were found. However, when analysing men and women separately, significant effects on gene expression were observed for women. Furthermore, when only including subjects with the highest and lowest serum 25(OH)D levels, additional vitamin D regulated genes were disclosed. Thus, a total of 99 genes (p≤0.05, log2 fold change ≥|0.2|) were found to be regulated, of which 72 have not been published before as influenced by vitamin D.
These genes were particularly involved in the

  • interleukin signaling pathway,
  • oxidative stress response,
  • apoptosis signaling pathway and
  • gonadotropin releasing hormone receptor pathway.

Thus, our results open the possibility for many future studies.

Copyright © 2017 Elsevier Ltd. All rights reserved. PMID: 28330721 DOI: 10.1016/j.jsbmb.2017.03.016
PDF is available at https://www.deepdyve.com

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