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Migranes not reduced by those having a fraction of a nanogram more vitamin D (no surprise) – Jan 2014

Vitamin D Status in Migraine Patients: A Case-Control Study

BioMed Research International; Volume 2014 (2014), Article ID 514782, 7 pages; http://dx.doi.org/10.1155/2014/514782
Alireza Zandifar,1,2 Samaneh sadat Masjedi,2 Mahboobeh Banihashemi,2 Fatemeh Asgari,2 Navid Manouchehri,2 Homa Ebrahimi,3 Faraidoon Haghdoost,1,2 and Mohammad Saadatnia1,4

1 Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran
2 Medical Student Research Center, Isfahan University of Medical Sciences, Isfahan 81745-319, Iran
3 Medical Student Research Center, Faculty of Medicine, Islamic Azad University, NajafAbad Branch, NajafAbad 8514143131, Iran
4 Department of Neurology and Isfahan Neurosciences Research Center, Isfahan University of Medical Sciences, Hezarjarib Avenue, Isfahan 81745-319, Iran

Background. There have been few studies on the relation between vitamin D and migraine. We investigated the prevalence of vitamin D deficiency in migraine patients and compared it with a control group. We also evaluated the relationship of vitamin D deficiency with severity of migraine. Methods. 105 newly diagnosed migraine patients and 110 controls, matched for age, sex, socioeconomic status, education, and sun exposure, were enrolled during the spring of 2011. 25-Hydroxy vitamin D [25(OH)D] plasma levels were measured by chemiluminescence immunoassay.
Results. The mean ± SE concentration of 25(OH)D was  13.5 ng/mL in cases and  13.2 ng/mL in controls. There was no significant difference in 25(OH)D concentration between cases and controls. We found no relationship between severity of headache and 25(OH)D status.

Conclusions. We did not find any association between migraine and vitamin D status; also, severity of headaches was not related to 25(OH)D level. Further studies with larger sample sizes are required to confirm our results.

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How could they possibly think that 1/3 nanogram more of vitamin D could possibly make any difference to anything?

See also VitaminDWiki

A later paper which commented on the many ways the original paper ignored mechanisms which could have lowered vitamin D levels

Comment on “Vitamin D Status in Migraine Patients: A Case-Control Study
BioMed Research International; Volume 2014 (2014), Article ID 635491, 2 pages, http://dx.doi.org/10.1155/2014/635491
Fevzi Nuri Aydin,1 Ibrahim Aydin,2 and Mehmet Agilli3
1Department of Biochemistry, Sirnak Military Hospital, 73000 Sirnak, Turkey
2Department of Biochemistry, Sarikamis Military Hospital, Sarikamis, 36500 Kars, Turkey
3Department of Biochemistry, Agri Military Hospital, 04000 Agri, Turkey
Received 10 March 2014; Accepted 19 March 2014; Published 31 March 2014

We read with great interest the article by Alireza Zandifar et al. entitled “Vitamin D status in migraine patients: a case-control study” [1], in which they report no association between migraine and vitamin D status. However, we think that some points should be discussed.

In humans, vitamin D is derived mainly from the action of sunlight on the skin. Some factors alter the cutaneous production of vitamin D, such as age, melanin, sunscreens, covered dresses, drugs, time of the day, latitude, and glass. Sensible sun exposure (usually 5–10 minutes of exposure of the arms and legs or the hands, arms, and face, 2 or 3 times per week) protects body against vitamin D deficiency. Vitamin D deficiency is any serum 25(OH)D result less than 20 ng/mL. In this study, duration of sun exposure was mentioned as 120 min/day or ≥120 min/day. Although time is enough for synthesis, the mean level of plasma 25-hydroxyvitamin D (25(OH)D) was  ng/mL in cases and  ng/mL in controls. Women, Iranian people, who wore covered dresses were most likely to have low vitamin D levels [2]. These factors could have affected the results of the study. The frequency of female patients wearing covered dresses should have been mentioned.

UV-B radiation does not penetrate glass, so exposure to sunshine indoors through a window does not produce 25(OH)D [2]. The environmental conditions of the subjects in this study should be presented.

Several vitamin D metabolites are found in cerebral spinal fluid and have the ability to cross the blood-brain barrier. These vitamin D metabolites include 25(OH)D3, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), and 24,25-dihydroxyvitamin D3. Addition of these metabolites in the study could show in more detail the relationship between migraine and vitamin D [3].

A 70-year-old person exposed to the same amount of sunlight as a 20-year-old person makes 25% of the vitamin D that the 20-year-old person can make. Older adults have a reduced level of 7-dehydrocholesterol, so they cannot synthesize 25(OH)D as well. Furthermore, their kidneys are less able to produce the active hormone, 1,25(OH)2D [2]. In this study, the distribution of age groups should be mentioned more clearly.

Melanin in the darker skin reduces the ability to produce 25(OH)D from sunlight exposure, because it absorbs the sunlight [2]. The frequency of dark-skinned subjects should be mentioned in the study.

Vitamin D is fat-soluble; it is sequestered in the body fat not allowing it to circulate. Moreover, those who have obesity cannot absorb vitamin D as readily [4]. Obesity is not among the exclusion criteria of the study; because of this, the results may have been influenced. Obesity should be stated in exclusion criteria in this study.

An inverse relationship between serum 25(OH)D and serum parathyroid hormone (PTH) is well known. When 25(OH)D levels are over 30 ng/mL, PTH concentration levels drop. If 25(OH)D concentrations are reduced to between 20 and 29 ng/mL, PTH concentration increases. In this study, PTH levels in case and control groups were not examined. Furthermore, hyperthyroidism and hypothyroidism can seriously affect the levels of vitamin D [5].

Recently, 25 experts from various medical disciplines drafted recommendations for vitamin D. As a result, serum is the recommended sample type. EDTA and other anticoagulants might have affected the measurements of 25(OH)D especially in immunoassays. Examples would be more accurate when using serum instead of plasma [6].

Consequently, explanation of these factors mentioned above could provide the readers with clearer information.

Conflict of Interests: The authors certify that there is no actual or potential conflict of interests in relation to this paper.


  1. A. Zandifar, S. S. Masjedi, M. Banihashemi et al., “Vitamin D status in migraine patients: a case-control study,” BioMed Research International, vol. 2014, Article ID 514782, 7 pages, 2014. View at Publisher · View at Google Scholar
  2. M. F. Holick, “Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease,” The American Journal of Clinical Nutrition, vol. 80, no. 6, pp. 1678S–1688S, 2004. View at Scopus
  3. L. R. Harms, T. H. J. Burne, D. W. Eyles, and J. J. McGrath, “Vitamin D and the brain,” Best Practice and Research: Clinical Endocrinology and Metabolism, vol. 25, no. 4, pp. 657–669, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. K. Rajakumar, J. D. Fernstrom, M. F. Holick, J. E. Janosky, and S. L. Greenspan, “Vitamin D status and response to vitamin D3 in obese vs. Non-obese African American children,” Obesity, vol. 16, no. 1, pp. 90–95, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. M. F. Holick, “Vitamin D: a millenium perspective,” Journal of Cellular Biochemistry, vol. 88, no. 2, pp. 296–307, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. J.-C. Souberbielle, J.-J. Body, J. M. Lappe et al., “Vitamin D and musculoskeletal health, cardiovascular disease, autoimmunity and cancer: recommendations for clinical practice,” Autoimmunity Reviews, vol. 9, no. 11, pp. 709–715, 2010. View at Publisher · View at Google Scholar · View at Scopus

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