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Low Level Laser Therapy (PBM) helped by Vitamin D (dental stem cells in this case) – June 2021

Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells

J Lasers Med Sci. 2021; 12: e30.Published online 2021 Jun 24. doi: 10.34172/jlms.2021.30
Latifa M. Abdelgawad, 1 ,* Nehal Salah, 2 Dina Sabry, 3 and Marwa Abdelgwad 4

VitaminDWiki

I designed and made an LLLT device before founding VitaminDWiki
Have used LLLT and Vitamin D separately for many health problems
Have also used them together for various dental problems
My design uses extreme modulated (Recommended by Russin research)
Extreme modulation increases the benefit by ~100X
This study does not appear to have any modulation
 
Topical vitamin D and LLLT work best on problems within 1/2 of the surface

VitaminDWiki pages with LOW LEVEL LASER in title

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Introduction: The regeneration of dental pulp tissue using human dental pulp stem cells (HDPSCs) has attracted increasing attention in recent years. Recent studies have suggested that several factors such as photobiomodulation (PBM) and vitamin D affect the proliferation and differentiation of HDPSCs. Therefore, the present study evaluated the effects of PBM and vitamin D on odontogenic differentiation of HDPSCs for dentin -like tissue formation.

Methods: HDPSCs were collected, isolated, and characterized and then divided into six groups: group I, control; group II, vitamin D (10-7 Mol); group III, irradiation at 1 J/cm2 of 810 nm diode laser; group IV, irradiation at 1 J/cm2 and culture with vitamin D; group V, irradiation at 2 J/cm2, and group VI, irradiation at 2 J/cm2 and culture with vitamin D, cell viability assay was measured through MTT. Alkaline phosphatase (ALP) enzyme activity and mRNA levels of vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), and dentin sialophosphoprotein (DSPP) were also assessed.

Results: PBM at 1 and 2 J/cm2 combined with vitamin D significantly promoted HDPSCs proliferation through MTT assay and odontogenic differentiation through gene expression of VEGF, BMP-2, and DSPP levels (P < 0.0001).

Conclusion: PBM at 2 J/cm2 combined with vitamin D enhanced the HDPSCs proliferation and odontogenic differentiation and thus could be a novel strategy for dentin regeneration in dentistry.


Created by admin. Last Modification: Saturday November 6, 2021 16:21:51 GMT-0000 by admin. (Version 8)

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16546 LLLT plus Vit D help dental pulp cells.pdf PDF 2021 admin 06 Nov, 2021 15:59 1.01 Mb 21
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