Loading...
 
Translate Register Log In Login with facebookLogin and Register

Leprosy 28 X more likely if a newly found Vitamin D Receptor modification (A61894G) – Dec 2017


A novel vitamin D receptor polymorphism associated with leprosy - Dec 2017

Journal of Dermatological Science, online 23 December 2017 https://doi.org/10.1016/j.jdermsci.2017.12.007

Highlights from PDF
 A new polymorphism, A61894G, in VDR gene was associated to leprosy
 The transversion (G>A) conferred susceptibility for disease in PB group
 T61968C and A61894G polymorphisms interaction are crucial in M. leprae infection
 TCAA genotype and negative Mitsuda presented 28.33-fold chance to develop the leprosy
 Download the PDF from Sci-Hub via VitaminDWiki

Chance of leprosy occurrence (odds ratio) by association of VDR gene polymorphisms and Mitsuda test results in patients and household contacts
Image
Leprosy appears to also be related to many of the other VDR gene polymorphisms


Leprosy 3X more likely if poor VDR – Nov 2018

VDR polymorphism, gene expression and vitamin D levels in leprosy patients from North Indian population
PLOS x, Nov 27, 2018 https://doi.org/10.1371/journal.pntd.0006823
Itu Singh , Mallika Lavania, Vinay Kumar Pathak , Madhvi Ahuja , Ravindra P. Turankar, Vikram Singh, Utpal Sengupta

Background
Leprosy is a chronic infectious disease caused by Mycobacterium leprae and mainly affects skin, peripheral nerves. Vitamin D receptor (VDR) gene polymorphism has been found to be associated with leprosy. Vitamin D has been shown to control several host immunomodulating properties through VDR gene. Vitamin D deficiency was also found to be linked to an increased risk for several infections and metabolic diseases.

Objective
In the present study, we investigated the association of VDR gene polymorphism, mRNA gene expression of VDR and the vitamin D levels with leprosy and its reactional states.

Methodology
A total of 305 leprosy patients consisting of tuberculoid (TT), borderline tuberculoid (BT), borderline lepromatous (BL), lepromatous leprosy (LL), as well as 200 healthy controls were enrolled in the study. We identified single nucleotide polymorphisms (SNPs) of VDR Taq1, Fok1 and Apa1, as well as the expression of VDR mRNA gene using PCR-based restriction fragment length polymorphism (RFLP) analysis and real-time PCR respectively. We also performed ELISA to measure vitamin D levels.

Result
We observed that SNP of VDR gene (Fok1 and Taq1) are associated with the leprosy disease. The allelic frequency distribution of T and t allele (p = 0.0037), F and f allele (p = 0.0024) was significantly higher in leprosy patients and healthy controls. ff genotype of Fok1 was found to be associated with leprosy patients [p = 0.0004; OR (95% CI) 3.148 (1.662–5.965)]. The recessive model of Fok1 genotype was also found to be significantly associated in leprosy patients in comparison to healthy controls [p = 0.00004; OR (95% CI) 2.85 (1.56–5.22)]. Leprosy patients are significantly associated with t-F-a haplotype. Further, VDR gene expression was found to be lower in non-reaction group compared to that of reaction group of leprosy and healthy controls. Paradoxically, we noted no difference in the levels of vitamin D between leprosy patients and healthy controls.

Conclusion
Blood levels of vitamin D do not play any role in clinical manifestations of any forms of leprosy. ff genotype of Fok1 and tt genotype of Taq1 was found to be associated with leprosy per se. Association of t-F-a haplotype with leprosy was found to be significant and could be used as a genetic marker to identify individuals at high risk for developing leprosy. VDR gene expression was lower in TT/BT and BL/LL groups of leprosy in comparison to that of healthy controls.

Author summary
Present study was carried out to find out the association of vitamin D receptor (VDR) gene polymorphism, mRNA gene expression of VDR gene and level of vitamin D with leprosy reactions and leprosy patients. Surprisingly, level of vitamin D in leprosy patients was not found to be associated with the disease and its manifestations. VDR genotypes (Fok1 and Taq1) were found to be associated with leprosy patients. t-F-a haplotype was significantly associated with leprosy patients. Gene expression of vitamin D receptor was lower in leprosy patients in comparison to healthy controls.


See also web (not about 2017 gene)

  • Leprosy Wikipedia
    infection by the bacterium Mycobacterium leprae or Mycobacterium lepromatosis.
    " Most new cases occur in 16 countries, with India accounting for more than half."
    Image
Created by admin. Last Modification: Tuesday November 27, 2018 21:54:44 UTC by admin. (Version 12)

Attached files

ID Name Comment Uploaded Size Downloads
10925 VDR Leprosy Nov 2018.pdf PDF 2018 admin 27 Nov, 2018 21:50 2.31 Mb 1
9013 WHO leprosy 2016.jpg admin 24 Dec, 2017 17:45 49.80 Kb 235
9010 VDR Leprosy.jpg admin 24 Dec, 2017 16:19 40.75 Kb 137
9009 VDR leprosy.pdf PDF 2017 admin 24 Dec, 2017 13:35 176.02 Kb 105
See any problem with this page? Report it (FINALLY WORKS)