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Eye vitamin D may not be associated with blood VitD, but is associated with CYP27B1 and CYP24A1 – Nov 2019

Intraocular Calcidiol: Uncovering a role for vitamin D in the eye.

J Steroid Biochem Mol Biol. 2019 Nov 14:105536. doi: 10.1016/j.jsbmb.2019.105536.

VitaminDWiki

Vision category starts with:

76 Vision and Vitamin D items

CYP27B1 category listing contains the following

The CYP27B1 gene activates Vitamin D in the Kidney,    Skin,    Lungs,    Brain,   Eyes   etc.
CYtochrome P450 family 27 subfamily B member 1    = 25-Hydroxyvitamin D3 1-alpha-hydroxylase

Vitamin D blood test misses CYP27B1 and other genes
Blood Test Misses a lot (VDW 3439)


Note: the Vitamin D levels in the abstract appear to be in error
Typical Vitamin D levels in blood are 50X to 500X larger
Hope to see the study PDF soon

Rullo J1, Pennimpede T2, Far PM3, Strube YN3, Irrcher I3, Urton T3, Bona M3, Gonder T3, Campbell R3, Ten Hove M3, Sharma S3, Farmer J4, Petkovich M5.
1 Queen's University, Department of Ophthalmology, Kingston, Ontario, Canada. jrullo at qmed.ca
2 Queen's University, Department of Laboratory and Molecular Pathology, Kingston, Ontario, Canada.
3 Queen's University, Department of Ophthalmology, Kingston, Ontario, Canada.
4 Queen's University, Department of Ophthalmology, Kingston, Ontario, Canada; +Dept of Laboratory and Molecular Pathology
5 Queen's University, Department of Biomedical and Molecular Sciences, Kingston, Ontario, Canada.

Vitamin D has emerged as a potentially important molecule in ophthalmology. To date, all ophthalmic data pertaining to vitamin D has been restricted primarily to tear and serum analysis in human patients. Considering the isolated nature of the eye, we sought to determine the presence of intraocular vitamin D in ocular disease.

METHODS:
25-hydroxyvitamin D3 (25(OH)D3) concentrations were measured in the eye and blood of 120 participants undergoing ophthalmic procedures. Ocular localization of the 1,25-dihydroxyvitamin D3-generating (CYP27B1) and deactivating (CYP24A1) hydroxylases was performed by immunohistochemistry. Gene expression of CYP27B1, CYP24A1 and VEGF-A was measured in eyes from patients with and without disease.

RESULTS:
25(OH)D3 was quantified in 112 ocular samples. In 40 cataract patient samples, the average 25(OH)D3 concentration was 0.057 ng/mL, compared to 72 retinal disease patient samples, average of 0.502 ng/mL (p < 0.001).
Intraocular 25(OH)D3 did not correlate with serum levels of 25(OH)D3.
There was no difference between the level of 25(OH)D3 measured in the aqueous and vitreous humour.
The vitamin D-specific CYPs 27B1 and 24A1, strongly localized to

  • complementary regions of the ciliary body,
  • retinal pigment epithelium and
  • neural retina.

Gene expression analysis confirmed retinal CYP27B1 correlated strongly with VEGF-A in eyes from diabetic patients (r = 0.92, p < 0.001).

CONCLUSIONS:
Our data confirms that vitamin D is present in the humours of the human eye and that local synthesis/degradation is possible via the ocular CYP27B1 and CYP24A1. This argues for a functional role for local vitamin D production and signaling in the eye and suggests that vitamin D may be an important intraocular mediator in disease pathogenesis.

Created by admin. Last Modification: Monday November 18, 2019 12:42:13 GMT-0000 by admin. (Version 5)
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