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Eye vitamin D may not be associated with blood VitD, but is associated with CYP27B1 and CYP24A1 – Nov 2019

Intraocular Calcidiol: Uncovering a role for vitamin D in the eye.

J Steroid Biochem Mol Biol. 2019 Nov 14:105536. doi: 10.1016/j.jsbmb.2019.105536.

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Rullo J1, Pennimpede T2, Far PM3, Strube YN3, Irrcher I3, Urton T3, Bona M3, Gonder T3, Campbell R3, Ten Hove M3, Sharma S3, Farmer J4, Petkovich M5.
1 Queen's University, Department of Ophthalmology, Kingston, Ontario, Canada. jrullo at qmed.ca
2 Queen's University, Department of Laboratory and Molecular Pathology, Kingston, Ontario, Canada.
3 Queen's University, Department of Ophthalmology, Kingston, Ontario, Canada.
4 Queen's University, Department of Ophthalmology, Kingston, Ontario, Canada; +Dept of Laboratory and Molecular Pathology
5 Queen's University, Department of Biomedical and Molecular Sciences, Kingston, Ontario, Canada.

Vitamin D has emerged as a potentially important molecule in ophthalmology. To date, all ophthalmic data pertaining to vitamin D has been restricted primarily to tear and serum analysis in human patients. Considering the isolated nature of the eye, we sought to determine the presence of intraocular vitamin D in ocular disease.

METHODS:
25-hydroxyvitamin D3 (25(OH)D3) concentrations were measured in the eye and blood of 120 participants undergoing ophthalmic procedures. Ocular localization of the 1,25-dihydroxyvitamin D3-generating (CYP27B1) and deactivating (CYP24A1) hydroxylases was performed by immunohistochemistry. Gene expression of CYP27B1, CYP24A1 and VEGF-A was measured in eyes from patients with and without disease.

RESULTS:
25(OH)D3 was quantified in 112 ocular samples. In 40 cataract patient samples, the average 25(OH)D3 concentration was 0.057 ng/mL, compared to 72 retinal disease patient samples, average of 0.502 ng/mL (p < 0.001).
Intraocular 25(OH)D3 did not correlate with serum levels of 25(OH)D3.
There was no difference between the level of 25(OH)D3 measured in the aqueous and vitreous humour.
The vitamin D-specific CYPs 27B1 and 24A1, strongly localized to

  • complementary regions of the ciliary body,
  • retinal pigment epithelium and
  • neural retina.

Gene expression analysis confirmed retinal CYP27B1 correlated strongly with VEGF-A in eyes from diabetic patients (r = 0.92, p < 0.001).

CONCLUSIONS:
Our data confirms that vitamin D is present in the humours of the human eye and that local synthesis/degradation is possible via the ocular CYP27B1 and CYP24A1. This argues for a functional role for local vitamin D production and signaling in the eye and suggests that vitamin D may be an important intraocular mediator in disease pathogenesis.


Created by admin. Last Modification: Tuesday July 7, 2020 10:40:02 GMT-0000 by admin. (Version 6)

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