Mol Nutr Food Res. 2019 Mar 28:e1801323. doi: 10.1002/mnfr.201801323.
Margier M1, Antoine T 1, Siriaco A 1, Nowicki M 1, Halimi C 1, Maillot M 2, Georgé S 3, Reboul E 1 Emmanuelle.Reboul at univ-amu.fr
1 INSERM, Aix Marseille Univ, C2VN, Marseille, France.
2 MS-Nutrition, Marseille, France.
3 CTCPA, Avignon.
This study analyzed Vitamin uptake in an artificial intestine
Founder of VitaminDWiki does not recall seeing that pulses restrict
the uptake of Vitamin D or Vitamin K
Wonder if pulses limit Vit D, Vit K in the real gut as well
The vitamins they tested were in olive oil
Suspect increased uptake if micelle or emulsion forms of Vitamins had been used instead
If this study is correct, oil-based Vitamin D should NOT be taken with a pulse meal
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- Air pollution, toxins, heavy metals and smoking each result in lower Vitamin D levels – Nov 2018
- Drugs which create deficiencies in Vitamin D, Vitamin K, Magnesium, Zinc, Iron, etc. – Sept 2017
- Reasons for low response to vitamin D
- Vitamin D bioavailability in the gut– review Sept 2017
- Oil-based Vitamin D3 has the worst bioavailability – April 2014
- 10 things about Beans BlueZones
- 4X more protein than from meat for the same cost
- Beans are the only food that can fit into both a vegetable and a protein category,
- Pulses in diet reduced lipids - meta-analysis - May 2014
"Effect of dietary pulse intake on established therapeutic lipid targets for cardiovascular risk reduction: a systematic review and meta-analysis of randomized controlled trials"
Download the PDF from VitaminDWiki
- Calories consumed in Brazil: 13% from pulses
Note: no references in Google Scholar to this study as of Oc 2019
Wonder that a, b mean? - could not quickly find in the PDF
It is widely advised to increase pulse consumption. However, pulses are rich in molecules displaying lipid-lowering properties, including fibers, phytates, saponins and tannins. We thus explored the effects of pulses on fat-soluble vitamin bioavailability.
We assessed vitamin A (β-carotene and retinyl-palmitate), vitamin E (α-tocopherol), vitamin D (cholecalciferol) and vitamin K (phylloquinone) bioaccessibility, i.e. micellarization after in vitro digestion of meals containing either potatoes (control), household-cooked or canned pulses. The obtained mixed micelles were delivered to Caco-2 cells to evaluate vitamin uptake. We then specifically assessed the impact of fibers, phytates, saponins and tannins on both phylloquinone (used as a model vitamin) bioaccessibility and uptake.
The presence of pulses significantly decreased both vitamin
bioaccessibility (up to
- -65% for β-carotene,
- -69% for retinyl-palmitate,
- -45% for cholecalciferol,
- - 53% for α-tocopherol and
- -67% for phylloquinone) and
- -40% for retinyl-palmitate,
- -67% for cholecalciferol,
- -50% for α-tocopherol and
- -57% for phylloquinone).
Effects on bioaccessibility, but not on uptake, were dependent on pulse cooking method. Phylloquinone bioaccessibility was specifically impacted by saponins, tannins and fibers while its uptake was impacted by saponins, fibers and phytates.
Pulses can alter fat-soluble micronutrient bioavailability. Pulses should thus be cooked appropriately and consumed within micronutrient-rich meals. This article is protected by copyright. All rights reserved.
Clipped from PDF
- "It has also been shown that regular consumption of pulses can lead to decreased plasma cholesterol and triglyceride levels , suggesting an impact on lipid absorption and/or metabolism. This impact is likely due to pulse fiber content , but other pulse bioactive compounds such as phytates, saponins or polyphenols may also play important roles in this phenomenon "
- "Fat-soluble vitamin bioaccessibility and uptake are negatively correlated to the presence of fibers, phytates, saponins or tannins"
- "Pulses components are acknowledged to impair mineral bioavailability , as well as to reduce lipid absorption 14"
- Wiseman, E. M., Bar-El Dadon, S., Reifen, R., The vicious cycle of vitamin a deficiency: A review. Crit Rev Food Sci Nutr 2017, 57, 3703-3714.
- Arabi, A., El Rassi, R., El-Hajj Fuleihan, G., Hypovitaminosis D in developing countries-prevalence, risk factors and outcomes. Nat Rev Endocrinol 2010, 6, 550-561.
- Dror, D. K., Allen, L. H., Vitamin E deficiency in developing countries. Food and nutrition bulletin 2011, 32, 124-143.
- McNinch, A., Vitamin K deficiency bleeding: early history and recent trends in the United Kingdom. Early Hum Dev 2010, 86 Suppl 1, 63-65.
- Hilger, J., Goerig, T., Weber, P., Hoeft, B., Eggersdorfer, M., Carvalho, N. C., Goldberger, U., Hoffmann, K., Micronutrient Intake in Healthy Toddlers: A Multinational Perspective. Nutrients 2015, 7, 6938-6955.
- Roman Vinas, B., Ribas Barba, L., Ngo, J., Gurinovic, M., Novakovic, R., Cavelaars, A., de Groot, L. C., van't Veer, P., Matthys, C., Serra Majem, L., Projected prevalence of inadequate nutrient intakes in Europe. Annals of nutrition & metabolism 2011, 59, 84-95.
- Holick, M. F., The vitamin D deficiency pandemic: Approaches for diagnosis, treatment and prevention. Rev Endocr Metab Disord 2017, 18, 153-165.
- Azzi, A., Meydani, S. N., Meydani, M., Zingg, J. M., The rise, the fall and the renaissance of vitamin E. Arch Biochem Biophys 2016, 595, 100-108.
- Reboul, E., Absorption of vitamin A and carotenoids by the enterocyte: focus on transport proteins. Nutrients 2013, 5, 3563-3581.
- Reboul, E., Intestinal absorption of vitamin D: from the meal to the enterocyte. Food Funct 2015, 6, 356-362.
- Reboul, E., Vitamin E Bioavailability: Mechanisms of Intestinal Absorption in the Spotlight. Antioxidants (Basel) 2017, 6.
- Gan, Y., Hamel, C., O'Donovan, J. T., Cutforth, H., Zentner, R. P., Campbell, C. A., Niu, Y., Poppy, L., Diversifying crop rotations with pulses enhances system productivity. Sci Rep 2015, 5, 14625.
- Bouchenak, M., Lamri-Senhadji, M., Nutritional quality of legumes, and their role in cardiometabolic risk prevention: a review. J Med Food 2013, 16, 185-198.
- Anderson, J. W., Major, A. W., Pulses and lipaemia, short- and long-term effect: Potential in the prevention of cardiovascular disease. British Journal of Nutrition 2007, 88, 263-271.
- Asif, M., Rooney, L. W., Ali, R., Riaz, M. N., Application and opportunities of pulses in food system: a review. Crit Rev Food Sci Nutr 2013, 53, 1168-1179.
- Liu, N., Ru, Y., Wang, J., Xu, T., Effect of dietary sodium phytate and microbial phytase on the lipase activity and lipid metabolism of broiler chickens. Br J Nutr 2010, 103, 862-868.
- Han, L. K., Zheng, Y. N., Xu, B. J., Okuda, H., Kimura, Y., Saponins from platycodi radix ameliorate high fat diet-induced obesity in mice. J Nutr 2002, 132, 2241-2245.
- Zhao, H. L., Sim, J. S., Shim, S. H., Ha, Y. W., Kang, S. S., Kim, Y. S., Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction. Int J Obes (Lond) 2005, 29, 983-990.
- Chavez-Santoscoy, R. A., Gutierrez-Uribe, J. A., Serna-Saldivar, S. O., Effect of flavonoids and saponins extracted from black bean (Phaseolus vulgaris L.) seed coats as cholesterol micelle disruptors. Plant foods for human nutrition 2013, 68, 416-423.
- Margier, M., George, S., Hafnaoui, N., Remond, D., Nowicki, M., Du Chaffaut, L., Amiot, M. J., Reboul, E., Nutritional Composition and Bioactive Content of Legumes: Characterization of Pulses Frequently Consumed in France and Effect of the Cooking Method. Nutrients 2018, 10.
- Malapert, A., Tomao, V., Margier, M., Nowicki, M., Gleize, B., Dangles, O., Reboul, E., beta- Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds. Nutrients 2018, 10.
- Reboul, E., Richelle, M., Perrot, E., Desmoulins-Malezet, C., Pirisi, V., Borel, P., Bioaccessibility of carotenoids and vitamin E from their main dietary sources. J Agric Food Chem 2006, 54, 8749-8755.
- Reboul, E., Abou, L., Mikail, C., Ghiringhelli, O., Andre, M., Portugal, H., Jourdheuil-Rahmani, D., Amiot, M. J., Lairon, D., Borel, P., Lutein transport by Caco-2 TC-7 cells occurs partly by a facilitated process involving the scavenger receptor class B type I (SR-BI). Biochem J 2005, 387, 455-461.
- Goncalves, A., Margier, M., Tagliaferri, C., Lebecque, P., George, S., Wittrant, Y., Coxam, V., Amiot, M. J., Reboul, E., Pinoresinol of olive oil decreases vitamin D intestinal absorption. Food Chem 2016, 206, 234-238.
- Reboul, E., Goncalves, A., Comera, C., Bott, R., Nowicki, M., Landrier, J. F., Jourdheuil-Rahmani, D., Dufour, C., Collet, X., Borel, P., Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. Mol Nutr Food Res 2011, 55, 691-702.
- Gleize, B., Steib, M., Andre, M., Reboul, E., Simple and fast HPLC method for simultaneous determination of retinol, tocopherols, coenzyme Q(10) and carotenoids in complex samples. Food Chem 2012, 134, 2560-2564.
- Sandberg, A. N., Bioavailability of minerals in legumes. British Journal of Nutrition 2002, 33, S281-285.
- Marlett, J. A., Content and composition of dietary fiber in 117 frequently consumed foods. J Am Diet Assoc 1992, 92, 175-186.
- Vahouny, G. V., Tombes, R., Cassidy, M. M., Kritchevsky, D., Gallo, L. L., Dietary fibers: V. Binding of bile salts, phospholipids and cholesterol from mixed micelles by bile acid sequestrants and dietary fibers. Lipids 1980, 15, 1012-1018.
- Riedl, J., Linseisen, J., Hoffmann, J., Wolfram, G., Some dietary fibers reduce the absorption of carotenoids in women. J Nutr 1999, 129, 2170-2176.
- Kies, A. K., De Jonge, L. H., Kemme, P. A., Jongbloed, A. W., Interaction between protein, phytate, and microbial phytase. In vitro studies. J Agric Food Chem 2006, 54, 1753-1758.
- Gu, Y., Hurst, W. J., Stuart, D. A., Lambert, J. D., Inhibition of key digestive enzymes by cocoa extracts and procyanidins. J Agric Food Chem 2011, 59, 5305-5311.
- Oliveira, R. F., Goncalves, G. A., Inacio, F. D., Koehnlein, E. A., de Souza, C. G., Bracht, A., Peralta, R. M., Inhibition of Pancreatic Lipase and Triacylglycerol Intestinal Absorption by a Pinhao Coat (Araucaria angustifolia) Extract Rich in Condensed Tannin. Nutrients 2015, 7, 5601-5614.
- Desmarchelier, C., Margier, M., Preveraud, D. P., Nowicki, M., Rosilio, V., Borel, P., Reboul, E., Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25- Hydroxycholecalciferol and 1-alpha-Hydroxycholecalciferol. Nutrients 2017, 9.
- Goncalves, A., Margier, M., Roi, S., Collet, X., Niot, I., Goupy, P., Caris-Veyrat, C., Reboul, E., Intestinal scavenger receptors are involved in vitamin K1 absorption. J Biol Chem 2014, 289, 3074330752.
- Borel, P., Lietz, G., Goncalves, A., Szabo de Edelenyi, F., Lecompte, S., Curtis, P., Goumidi, L., Caslake, M. J., Miles, E. A., Packard, C., Calder, P. C., Mathers, J. C., Minihane, A. M., Tourniaire, F., Kesse-Guyot, E., Galan, P., Hercberg, S., Breidenassel, C., Gonzalez Gross, M., Moussa, M.,#Meirhaeghe, A., Reboul, E., CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans. J Nutr 2013, 143, 448-456.
- Reboul, E., Trompier, D., Moussa, M., Klein, A., Landrier, J. F., Chimini, G., Borel, P., ATP-binding cassette transporter A1 is significantly involved in the intestinal absorption of alpha- and gamma- tocopherol but not in that of retinyl palmitate in mice. Am J Clin Nutr 2009, 89, 177-184.
- Goncalves, A., Gleize, B., Roi, S., Nowicki, M., Dhaussy, A., Huertas, A., Amiot, M. J., Reboul, E., Fatty acids affect micellar properties and modulate vitamin D uptake and basolateral efflux in Caco-2 cells. J Nutr Biochem 2013, 24, 1751-1757.
- Goncalves, A., Gontero, B., Nowicki, M., Margier, M., Masset, G., Amiot, M. J., Reboul, E., Micellar lipid composition affects micelle interaction with class B scavenger receptor extracellular loops. J Lipid Res 2015, 56, 1123-1133.
- Jenkins, K. J., Atwal, A. S., Effects of dietary saponins on fecal bile acids and neutral sterols, and availability of vitamins A and E in the chick. The Journal of Nutritional Biochemistry 1994, 5, 134-137.
- Johnson, I. T., Gee, J. M., Price, K., Curl, C., Fenwick, G. R., Influence of saponins on gut permeability and active nutrient transport in vitro. J Nutr 1986, 116, 2270-2277.
- Reboul, E., Thap, S., Perrot, E., Amiot, M. J., Lairon, D., Borel, P., Effect of the main dietary antioxidants (carotenoids, gamma-tocopherol, polyphenols, and vitamin C) on alpha-tocopherol absorption. Eur J Clin Nutr 2007.
- Reboul, E., Thap, S., Tourniaire, F., Andre, M., Juhel, C., Morange, S., Amiot, M. J., Lairon, D., Borel, P., Differential effect of dietary antioxidant classes (carotenoids, polyphenols, vitamins C and E) on lutein absorption. Br J Nutr 2007, 97, 440-446.
- Miura, S., Saku, K., Ezetimibe, a selective inhibitor of the transport of cholesterol. Intern Med 2008, 47, 1165-1170.
- Shen, W. J., Hu, J., Hu, Z., Kraemer, F. B., Azhar, S., Scavenger receptor class B type I (SR-BI): a versatile receptor with multiple functions and actions. Metabolism 2014, 63, 875-886.
- Bohn, T., Dietary factors affecting polyphenol bioavailability. Nutr Rev 2014, 72, 429-452.