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Cancer incidence and mortality is decreased if 40-60 ng of Vitamin D – April 2019

Vitamin D3 from Ultraviolet-B Exposure or Oral Intake in Relation to Cancer Incidence and Mortality
Current Nutrition Reports https://doi.org/10.1007/s13668-019-0262-5
William B. Grant, Meis Moukayed
William B. Grant, wbgrant at infionline.net; https://www.sunarc.org, Sunlight, Nutrition, and Health Research Center, P.O. Box 641603, San Francisco, CA 94164-1603, USA
Meis Moukayed mmoukayed at hotmail.com, School of Arts and Sciences, American University in Dubai, P.O. Box 28282, Dubai, UAE


Cancer category starts with the following

Cancers get less Vitamin D when there is a poor Vitamin D Receptor

Vitamin D Receptor and Cancers lightly mentiond by this study
81+ Vitamin D Receptor pages with CANCER in the title
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Items found: 83
Title Modified
Cervical Cancer is prevented by Vitamin D, and treated if caught early (genes are important) – May 2023 27 May, 2023
Colon Cancer protects itself by changing the VDR and CYP3A4 genes – Dec 2022 06 Dec, 2022
Breast cancer spreads to bone if poor vitamin D Receptor (no surprise) – Oct 2022 31 Oct, 2022
2X more Thyroid Cancer malignancy if less than 15 ng of vitamin D – June 2012 16 Sep, 2022
Cancers are associated with low vitamin D, poor vaccination response and perhaps poor VDR – July 2022 15 Aug, 2022
Poor prognosis of solid childhood cancers 14.7 X more likely with a poor Vitamin D Receptor – July 2022 27 Jul, 2022
Breast Cancer, Vitamin D, and genes – Welsh Nov 2021 09 Feb, 2022
How cancer is fought by Vitamin D (Ovarian this time) - Feb 2020 01 Nov, 2021
Lung Cancer is up to 7 X more deadly if poor vitamin D genes – Oct 2021 25 Oct, 2021
Cancers and Vitamin D Receptors, including change with race – Feb 2021 24 Oct, 2021
Breast Cancer reduces receptor and thus blocks Vitamin D to the cells – several studies 18 Oct, 2021
After lactation Vitamin D levels are low, increased risk of Breast Cancer, vitamin D should decrease risk – Aug 2021 20 Aug, 2021
Breasts process Vitamin D and change gene activation, might prevent breast cancer if given more Vit. D – July 2021 10 Jul, 2021
Vitamin D Receptor and Cancer 17 Feb, 2021
Oral Cancers - increased risk if low vitamin D or poor vitamin D genes 22 Jan, 2021
Colon cancer risk increases 30X if you have the worst vitamin D receptor mutation – Jan 2021 15 Jan, 2021
Book: Sunlight, UV, Vitamin D and Receptor, Skin and other Cancers - Dec 2020 09 Dec, 2020
Colorectal Cancer Patients 2.4 X more likely to have poor Vitamin D receptors (less D to cells) – April 2020 02 May, 2020
Colorectal cancer linked to poor Vitamin D Receptor (yet again) – Jan 2020 22 Jan, 2020
8 ways that Cancer might be prevented by Vitamin D - June 2019 26 Dec, 2019
Liver Cancer – higher risk if poor genes (Vitamin D receptor etc) – meta-analysis Dec 2019 14 Dec, 2019
Prostate Cancer associated with various genes, including Vitamin D Receptor and CYP24A1 – Nov 2019 15 Nov, 2019
Risk of Cancer increased if poor Vitamin D Receptor – meta-analysis of 73 studies Jan 2016 09 Nov, 2019
Cancer is leading cause of death - Vitamin D and Receptor activators help 09 Nov, 2019
Melanoma cancer growth slowed by increased Vitamin D Receptor (yet again) – Oct 2019 06 Nov, 2019
Breast cancer associated with Vitamin D Receptor (14th study) – Oct 2019 19 Oct, 2019
Colorectal Cancer risk increases when genes reduce the vitamin D levels – Aug 2019 23 Aug, 2019
Breast Cancer death 1.8 X more likely if poor Vitamin D Receptor – April 2019 29 Jul, 2019
Multiple Myeloma (blood cell cancer) treated by vitamin D - many studies 14 Jun, 2019
Blood cell cancer is associated with a 3X worse Vitamin D Receptor – June 2019 14 Jun, 2019
Lung Cancer more likely if poor Vitamin D Receptor – meta-analysis June 2019 13 Jun, 2019
After breast cancer treatment 4,000 IU of Vitamin D was not enough to help if have poor Vitamin D receptor – June 2019 04 Jun, 2019
Effects of Resveratrol against Lung Cancer in mice – Nov 2017 28 Apr, 2019
The Role of Resveratrol in Cancer Therapy – Dec 2017 27 Apr, 2019
Good Vitamin D receptor reduced bladder cancer and cisplatin deaths – April 2019 11 Apr, 2019
A poor Vitamin D Receptor is associated with many cancers (oral cancer in this case) – Jan 2019 26 Mar, 2019
Breast Cancer and Vitamin D review – March 2018 17 Mar, 2019
Overview of Vitamin D Actions in Cancer – 31 page chapter in a book – 2018 15 Mar, 2019
Women with Breast Cancer were 16.9 times more likely to have a poor Vitamin D Receptor – Jan 2019 11 Feb, 2019
Risks of Colorectal Cancer, IBD, etc slightly increased if poor Vitamin D Receptor – Aug 2018 09 Dec, 2018
Ovarian Cancer risk reduced if higher vitamin D, more UVB, or better vitamin D receptor – Nov 2018 10 Nov, 2018
Cancer treatment by Vitamin D sometimes is restricted by genes – Oct 2018 01 Nov, 2018
Brain cancer in 175 countries related to low UVB and low vitamin D – Oct 2010 03 Oct, 2018
Pancreatic Cancer – live a year longer if have high vitamin D and good Vitamin D Receptor – Aug 2018 15 Aug, 2018
Endometriosis, Endometrial Cancer, and poor Vitamin D or Receptor – Aug 2018 08 Aug, 2018
Risk of colon cancer increases in mice with no Vitamin D receptor in colon - July 2018 07 Jul, 2018
Vitamin D receptor is essential for both normal and cancerous cells in the lab – June 2018 16 Jun, 2018
Active Vitamin D reduces Ovarian Cancer stem cells growth by 4X (via Vitamin D receptor in lab rat) – March 2018 28 Mar, 2018
Two chemicals increase the Vitamin D receptor and decrease the growth of breast cancer cells in the lab - March 2018 17 Mar, 2018
Ovarian Cancer in Asia is 1.5 X more likely if poor Vitamin D receptor – meta-analysis Dec 2017 14 Dec, 2017
Nonmelanoma Skin Cancer 2X more likely if poor Vitamin D Receptor – Oct 2017 19 Oct, 2017
Cancer and the Vitamin D Receptor, a primer – Sept 2017 01 Oct, 2017
Thyroid Cancer rate has increased 3X in 3 decades, Vitamin D Receptor decreases, Calcium increases – Aug 2017 26 Aug, 2017
Advanced Colon Cancer risk is doubled or halved with 1000 IU of Vitamin D, depends on Vitamin D Receptors – RCT May 2017 19 Aug, 2017
Cancer risks and Vitamin D Receptors – association is unclear – 2017 19 Aug, 2017
Increased Breast Cancer metastasis if low vitamin D or poor VDR – Feb 2016 09 Aug, 2017
Colon Cancer survival 3.1 X less likely if poor Vitamin D Receptor – Aug 2017 02 Aug, 2017
Lung Cancer patients were 2.4 times more likely to have a poor Vitamin D Receptor gene – July 2017 27 Jul, 2017
Pancreatic Cancer massively deregulates the local Vitamin D receptors and CPY24A1 – July 2014 25 Jul, 2017
Gastric Cancer 2.7 X more likely if poor Vitamin D Receptor (Chinese) – 2015 15 Jun, 2017
Skin Cancers, Vitamin D, Vitamin D Receptor and Genes – Jan 2015 17 Mar, 2017
Prostate cancer in black men is 1.6 times more likely if a poor Vitamin D Receptor – Feb 2017 06 Mar, 2017
2X more likely to survive a form of esophageal cancer in China if have good vitamin D receptor – Feb 2017 04 Mar, 2017
Urinary Bladder Cancer survival is associated with vitamin D receptor: 14 months vs 53 months – Oct 2015 02 Mar, 2017
Vitamin D receptor as a target for breast cancer therapy (abstract only) – Feb 2017 01 Mar, 2017
Ovarian Cancer 5.8 X more likely if both low vitamin D and Fok1 gene change – May 2013 27 Feb, 2017
Vitamin D receptor may suppress skin cancer – Dec 2013 27 Jan, 2017
Aggressive Prostate Cancer in blacks with low vitamin D – 7X more likely if added Calcium – Jan 2017 28 Dec, 2016
Breast Cancer was 4.6 times more likely if have a poor Vitamin D Receptor – Dec 2016 06 Dec, 2016
Vitamin D, Vitamin D Receptor and Cancer – Nov 2016 05 Dec, 2016
High PSA readings with Prostate Cancer 4 times more likely if poor Vitamin D receptor – March 2016 14 Nov, 2016
2X less prostate cancer in A-A with low Calcium is due vitamin D receptor gene – July 2013 14 Nov, 2016
Vitamin D receptor polymorphisms are risk factors for various cancers – meta-analysis Jan 2014 12 Nov, 2016
10 percent of colon cancer linked to Vitamin D Receptor – meta-analysis April 2012 23 Sep, 2016
Vitamin D Receptor role in Autoimmune Diseases and or cancers – Nov 2013 17 Mar, 2016
Skin cancer 20 percent more likely with some Vitamin D receptor gene polymorphisms – Oct 2015 06 Jan, 2016
Pancreatic Cancer treatment by calcipotriol (a synthetic vitamin D) improves outcome by 57 percent – Sept 2014 13 Dec, 2015
Poor Bladder Cancer survival associated with poor Vitamin D receptor – Oct 2015 18 Oct, 2015
Increased risk of some female cancers if low vitamin D (due to genes) – meta-analysis June 2015 03 May, 2015
Free vitamin D (VDR) may be more important than total for bladder cancer – March 2013 06 Jan, 2014
Genes breast cancer and vitamin D receptor - Sept 2010 08 Jul, 2013
Breast Cancer incidence change by 40 percent with vitamin D receptor genes – Oct 2012 08 Jul, 2013
Vitamin D receptor in breasts and breast cancer vary with race – March 2013 08 Jul, 2013

Items in both categories Mortality and Cancer - Breast:

Items in both categories Mortality and Cancer - Colon:

Items in both categories Mortality and Cancer

 Download the PDF from VitaminDWiki

Purpose of Review This review summarizes the understanding of vitamin D3’s role in reducing risk of cancer incidence and mortality.

Recent Findings Recent randomized clinical trials and observational studies of participants who took part in vitamin D3 supplementation studies provide increasing evidence that concentrations of serum 25-hydroxyvitamin D3 [25(OH)D3] up to ~ 60 ng/ml are inversely correlated with all cancer and some specific cancers’ incidence and death, with a stronger effect on survival and death than on incidence. Mechanisms linking vitamin D3 to effects on cellular proliferation, anti-angiogenesis, and antimetastasis continue to be found.

Summary Vitamin D3 reduces cancer risk causally. Maintaining 25(OH)D3 in the range of 40-60 ng/ml reduces the risk of many cancers. Raising 25(OH)D3 concentrations after diagnosis to that range increases survival rates and could significantly reduce the global burden of cancer incidence and death.


The ultraviolet-B (UVB)—vitamin D—cancer hypothesis is nearing its 40th anniversary. Two beginning graduate students in public health proposed that hypothesis after seeing maps of cancer mortality rates and noticing an inverse correlation between colon cancer mortality rates and annual solar radiation exposure [1]. The hypothesis has received much support from other geographical ecological studies, observational studies, and studies of mechanisms [2-5]. However, in part, because of the concern that ecological and observational studies do not establish causality and because of limited support from randomized controlled trials (RCTs), the hypothesis has not received widespread support from the health care industry. With the results from some innovative observational studies and the most recent major vitamin D RCT, the support should increase.

Justification for Using Vitamin D in Cancer Prevention and Treatment

Rates of cancer incidence are rising globally. Despite increasing awareness and early detection and diagnosis for some cancers, the disease remains a major concern for populations around the world. First, treating the disease with surgery, radiotherapy, and chemotherapy is costly. Such treatments— many of which are pharmacological medications—are not easily accessible to 85% of the world population that lives in low- to middle-income countries. Second, metastatic cancers almost always recur a few years later. Therefore, in the global attempt to fight cancer, finding options that could simultaneously serve as preventive and therapeutic measures is imperative. Such options would prevent the disease from occurring by regulating the cellular clock of growth not only by maintaining healthy genes, cellular components, and molecular microenvironment but also by maintaining the mechanisms that clear cellular damage functional. To be therapeutic, such options must be able to:
Control the tumor microenvironment by blocking carcinogens or proliferation-inducing hormones at the cellular level,
Limit angiogenesis to block the tumor’s blood supply,
Reduce inflammation in the tumor microenvironment,
Permeate any resistant cancer stem cells in tumors, and
Prevent transformed cancer cells from migrating, docking at and invading neighboring tissues.
Surgery is the first-line treatment for 60% of solid tumors, but surgery almost always requires radiation therapy and chemotherapy to ensure that tumor cells have not remained behind to survive and metastasize. Radiation therapies and chemotherapies usually target at least one of the preceding mechanisms, but few radiotherapy treatments or chemotherapy agents have pleiotropic abilities that target all mechanisms of cellular transformation, malignancy, or metastasis. All such available physical or pharmacological therapies often have adverse side effects. As a public health measure, it may therefore be beneficial to find natural agents that are relatively cheap and accessible to the general public and that could prevent malignancy, block metastasis, and treat the disease, independently or as an adjuvant measure to existing therapies.
In light of those requirements, vitamin D shines as a stellar candidate in the search for an anticancer agent. It is both preventive and therapeutic as well as an inexpensive and readily accessible natural biochemical agent. Mounting evidence indicates that higher serum 25-hydroxyvitamin D concentrations [25(OH)D] reduce the incidence and mortality rate of cancer. Moreover, vitamin D’s multifaceted mechanisms of action can curtail several steps of cancerous transformation and malignancy simultaneously. Collectively, the body of evidence that we will present supports using vitamin D as a candidate in the global battle against cancer incidence, recurrence, and death.
This paper reviews recent advances in understanding how UVB exposure and vitamin D3 affect cancer incidence, progression, and mortality.

Types of Studies

Geographical ecological studies investigate correlations between cancer outcomes and indices of solar UVB doses. Such studies are best conducted in mid-latitude countries that have large variations in solar UVB doses but small variations in other cancer risk-modifying factors, which are generally included in the analysis. Such studies have identified about 20 cancers that have mortality rates inversely correlated with solar UVB doses [2]. That vitamin D has more impact on cancer progression and death than on incidence is becoming apparent [6], which may help explain why observational studies of cancer incidence rates have not supported the findings of ecological studies to the extent expected.
Observational studies generally monitor cohorts for several years and compare 25(OH)D measured at enrollment with cancer incidence. However, 25(OH)D changes with season and time in general, so the longer the follow-up time, the less useful that value is. As a result, researchers using case-control studies, in which 25(OH)D is measured near the time of cancer diagnosis, find stronger inverse correlations between 25(OH)D and cancer incidence [7]. Although case-control studies are not widely accepted as a result of the assumption that reverse causality may be involved because of cancer’s reduction of 25(OH)D, that assumption has not been verified and is probably incorrect. Findings from observational studies also indicate that 25(OH)D is directly correlated with survival rates for several cancers, including colorectal [8], lung [9], kidney, prostate, and melanoma [10], although lung cancer survival rate was poorer at higher 25(OH)D in Finland [10].
RCTs generally enroll people into a study in which half are randomly assigned to treatment such as vitamin D3 supplementation, sometimes with calcium, whereas the other half are assigned a placebo. Unfortunately, most vitamin D3 RCTs have been based on the model for pharmaceutical drugs, which assumes that the trial is the only source of the agent and that a linear dose-response relationship exists. Neither assumption holds for vitamin D3: increases in 25(OH)D3 decrease with vitamin D3 supplementation as baseline 25(OH)D3 increases, and 25(OH)D3-health outcome relationships are nonlinear, with greater changes in outcome for changes at lower 25(OH)D3. Other problems with vitamin D3 RCTs include enrolling people with relatively high 25(OH)D3, not measuring baseline or achieved 25(OH)D3, and giving low doses of vitamin D3. Vitamin D RCTs should be based on 25(OH)D, not vitamin D dose [11].
Two meta-analyses of RCTs investigating vitamin D supplementation and cancer incidence and mortality were reported recently. The first one included 24 studies of cancer incidence and 17 studies of cancer deaths [12]. The overall relative risk for cancer incidence from vitamin D treatment was 1.03 (0.91-1.15) while that for cancer death was 0.85 (0.70-1.04). The second one, published subsequently, used different criteria for inclusion and included only nine trials on cancer incidence and six trials on cancer death [13]. The overall relative risk for cancer incidence from vitamin D treatment was 0.99 (0.93-1.05) while that for cancer death was 0.88 (0.80-0.98). Although all of the RCTs included in both studies were based on vitamin D dose rather than 25(OH)D, so it probably does not represent the true benefit of vitamin D supplementation on reducing cancer incidence and death, they do show that vitamin D has a greater impact on cancer death than on cancer incidence.
Mendelian randomization (MR) studies investigate links between variations of genes that affect 25(OH)D3 and health outcomes such as cancer incidence. Such studies generally include the genes

  • CYP2R1, which codes for a key enzyme in converting vitamin D3 to 25(OH)D3;
  • CYP24A1, which destroys 1,25-dihydroxyvitamin D[1,25(OH)2D];
  • DHCR7, which converts 7-dehydrocholesterol to cholesterol; and
  • GC, which encodes the vitamin D binding protein.

Variations in alleles of those genes account for 1-4% of the variation in 25(OH)D [14]. Sometimes baseline 25(OH)D3 is measured. The correlation between cancer incidence and allele frequencies is used to determine whether vitamin D can be considered causally linked to the risk of particular cancers. To date, MR study results have been reported for incidence for many cancers. However, the only statistically significant result was for ovarian cancer in an Ovarian Cancer Association Consortium study involving 10,065 case patients and 21,654 control subjects of European ancestry, odds ratio (OR) = 1.27 (95% CI, 1.061.51) per 8 ng/ml decrease in 25(OH)D3 [15]. In that same paper, the OR for high-grade serous epithelial ovarian cancer as determined from 4121 cases was 1.54 (95% CI, 1.192.01). No significant results were found for prostate cancer in eight studies, in agreement with findings of observational studies [16]. In a more recent paper involving 1031 ovarian cancer cases, the MR OR involving 11,096 cases was 0.85 (95% CI, 0.73-1.00) [17]. In that same paper, results were not significant for incidence of any other cancer, possibly because the numbers of cases were small, or for mortality from breast, colorectal, lung, pancreatic, or prostate cancer. However, all cancer mortality rate was found inversely correlated with genetically determined 25(OH)D, OR per 8 ng/ml lower 25(OH)D= 1.43 (95% CI, 1.02-1.99) [18].
Trying to understand why MR studies generally find no significant correlations between genetically determined variations in 25(OH)D and cancer incidence is a worthwhile endeavor. One reason for the lack of such findings may be that the genetic factors explain at most about 3.5% of 25(OH)D [17]. A second reason is that some studies do not include enough cancer cases to have the statistical power to find a significant effect. A third reason is that 25(OH)D varies with respect to, for example, season, age, and supplementation. In an analysis of results for observational studies of breast and colorectal cancer, the longer the follow-up period after blood draw, the nearer the OR was to 1.0. The effect was stronger for breast than for colorectal cancer, which was attributed to the fact that breast cancer can develop rapidly [7, 19].

Observational Studies of 25(OH)D on Cancer Incidence

Colorectal Cancer In a meta-analysis of 15 prospective observational studies and one case-control study, researchers found a pooled OR of 0.67 (95% CI, 0.59-0.76) for colorectal cancer incidence for high versus low baseline 25(OH)D [20**]. When the ORs were averaged for each step of 10 ng/ml, a nearly linear inverse relationship emerged from <10 ng/ml to 50-60 ng/ml.
Breast Cancer The evidence that vitamin D reduces the risk of breast cancer incidence strengthened in 2018 because of findings from a pooled analysis of breast cancer incidence from two vitamin D RCTs (3325 women) and one observational study (1713 women) [21**]. Researchers measured 25(OH)D at baseline and after 1 year in the RCTs and every 6 months in the observational study. Participants in the RCTs took either 1100 or 2000 IU of vitamin D3 per day plus 1.5 g of calcium per day or placebo, whereas participants in the observational study freely chose their doses of vitamin D3 and calcium. Seventy-seven participants developed breast cancer during the 4- to 5-year study periods. A plot of breast cancer incidence rate versus 25(OH)D3 resulted in a nearly linear reduction from 762 cases/100,000 population for 25(OH)D < 20 ng/ml to 134 cases/100,000 for 25(OH)D > 60 ng/ml. The hazard ratio (HR) for > 60 ng/ml versus< 20 ng/ml was 0.20 (95% CI, 0.05-0.82); P = 0.03. One strength of that study was that 25(OH)D ranged up to > 60 ng/ml. The HR for 40-59 ng/ml versus < 20 ng/ml was 0.48 (95% CI, 0.20-1.14); P = 0.10. Another strength was that seasonal variations in 25(OH)D were reduced because more than half of the women were taking reasonably large doses of vitamin D3. It has been argued that case-control studies of breast cancer incidence were superior to prospective observational studies because breast cancer develops rapidly and both seasonal and long-term changes in 25(OH)D can reduce the long-term predictive value of 25(OH)D measured at baseline [7].
A recent cross-sectional study found that supplementing with vitamin D3 induces breast cancer- specific survival rate. The study was conducted in Ireland. Invasive breast cancer data for 5417 women aged 50-80 years were obtained from the National Cancer Registry Ireland database, and vitamin D supplement use was determined from national prescription data [22**]. For new vitamin D users, the HR was 0.80 (95% CI, 0.640.99); P = 0.048. For women who began taking vitamin D supplements within 6 months of diagnosis, the HR was 0.51 (95% CI, 0.34-0.74); P<0.001. Results were similar for users versus nonusers and users supplemented with > 400 IU/d versus nonusers and users supplemented with < 400 IU/d.
Prostate Cancer For prostate cancer, the relation with respect to solar UVB doses and 25(OH)D is different from that for most internal organ cancers. For example, in the USA, prostate cancer mortality rates were highest in the northwest and lowest in the southeast from 1950 to 1994, whereas mortality rates for breast, colon, and many other cancers are highest in the northeast and lowest in the southwest [23].
Findings from a meta-analysis of individual participant data in 19 prospective studies indicated higher versus lower 25(OH)D associated with increased risk of nonaggressive disease [adjusted OR= 1.22 (95% CI, 1.13-1.31); Ptrend <0.001] [24]. However, for aggressive disease, adjusted OR = 0.95 (95% CI, 0.78-1.15). In a meta-analysis of prostate cancer- specific mortality rate based on seven cohort studies, researchers found a HR of 0.91 (95% CI, 0.87-0.97; P = 0.01) per 8 ng/ml increase in 25(OH)D [17].
African-Americans (AAs) have much higher rates of prostate cancer than European Americans do: incidence rates are higher by a factor of 1.7, whereas mortality rate is higher by a factor of 2.4, according to data from 2008 to 2012 [25]. A recent paper reported results of a cross-sectional study of 2322 control subjects and men who underwent prostate cancer biopsy (consisting of 1381 AAs, 715 European Americans, and 226 from other racial/ethnic backgrounds), the data of which were later reduced to a case-control study of 1657 men (699 prostate cancer patients and 958 control subjects) [26**]. For AAs, the association between high versus low calcium intake and aggressive prostate cancer was ORQ1 vs. Q4 = 4.3 (95% CI, 1.7-10.8), whereas the association for high versus low vitamin D intake was ORq1 vs. q4 = 0.06 (95% CI, 0.02-0.54) [26**]. Similar findings for calcium and vitamin D intake were reported for men with body mass index (BMI) < 27.8 kg/m2 regardless of race but not for those with higher BMI.
Other Cancers Findings from recent observational studies also have indicated that 25(OH)D is inversely correlated with incidence of liver cancer in Europe [27] and Japan [28]. Reviews indicate that the evidence from observational studies for 25(OH)D are supportive for lung [9] and thyroid [29] cancer but weak for gynecological [30], (although supportive for ovarian cancer [31]), and pancreatic [32] cancers.

All-Cancer Incidence

In two recent observational studies, inverse correlations were found between 25(OH)D and all-cancer incidence [33**, 28]. One study that did not was from Germany, probably as a result of the low mean 25(OH)D (15.5 ng/ml) [34].
The results of a 5-year RCT does offer good evidence that vitamin D3 reduces risk of total cancer. The VITamin D and Omega-3TriAL (VITAL) enrolled more than 25,000 participants, more than 5000 of whom were Black [35**]. Enrollees were given 2000 IU/d vitamin D3 or placebo and 1 g/d omega-3 fatty acids or placebo. The mean baseline 25(OH)D was ~31 ng/ml and the baseline BMI was 28 kg/ m2. Participants were permitted to take up to 600 IU/d vitamin D3 (800 IU/d if older than 70 years). For the entire group, the HR for cancer incidence was 0.96 (95% CI, 0.88-1.06). However, for those with BMI <25 and < 27.1 kg/m2, the HR values were 0.76 (95% CI, 0.63-0.90) and 0.86 (95% CI, 0.75-0.99), respectively. The likely reason for a more beneficial effect for individuals with lower BMI is that the 2000 IU/d raises 25(OH)D more due to volumetric dilution with higher BMI [36]. Also, for Blacks, HR was 0.77 (95% CI, 0.59-1.01). According to that study’s online supplement, the mean 25(OH)D for Blacks was 25.0 ng/ml, rising to 39.7 ng/ml at year one on the basis of a sample size of 154. Baseline and achieved 25(OH)D for those with BMI < 25 kg/ m2 were 33.3 and 45.9 ng/ml, respectively, whereas achieved 25(OH)D was 41.4 and 38.6 ng/ml for 25 kg/m2 <BMI< 30 kg/m2 and BMI > 30 kg/m2, respectively. Thus, the subgroup results are consistent with the importance of both baseline and achieved 25(OH)D, indicating that the group had either baseline 25(OH)D been lower or the vitamin D3 dose higher, a significant reduction in all-cancer incidence would have been found. For the entire group, death from cancer was reduced non-statistically significantly in the treatment arm (HR = 0.83 [95% CI, 0.67-1.02]) but significantly in the analysis excluding the first 2 years (HR = 0.75 [95% CI, 0.590.96]).


From the evidence discussed here, as well as in other papers in the literature, keeping 25(OH)D in the range of at least 40-60 ng/ml would apparently provide optimal protection against most cancers. Vitamin D3 can be obtained through sensible sun exposure, avoiding erythema (sunburn), and through supplements. In a recent intervention study, having 25(OH)D > 40 ng/ml greatly reduced the development of erythema under UV irradiation [37].


Cell Proliferation, Survival, Apoptosis, and the Regulation of microRNAs

Cell proliferation, growth, and survival rely on several interlinked molecular signaling regulators that control the cell cycle and cellular turnover. In tumorigenesis, cell cycle control is dysfunctional, and the cell proliferates out of control in the absence of DNA repair rescue mechanisms, apoptosis, or autophagy—processes that otherwise would sweep up faulty or worn-out cells. Several studies strongly support evidence that 1,25(OH)2D3 or its analogues can suppress irregular cell proliferation, promote apoptosis, or activate cellular autophagy [38]. Findings in several cancer cell types collectively confirm that 1,25(OH)2D3 has an effective preventive role in tumorigenesis by regulating different signaling pathways and stages of the cell cycle.
Treatment of HeLaS3 cervical cancer cells with 1,25(OH)2D3 arrests the cell cycle at G1 phase and increases expression of the tumor suppressor gene p21. This is associated with a repression in mRNA expression and protein levels of the human cervical cancer oncogene HCCR-1 [39].
Treating MCF-7 breast cancer cell lines with 1,25(OH)2D3 induces cell cycle arrest at the G1/S transition phase by downregulating the synthesis or activity of cyclin and cyclin- dependent kinase (cdk) complexes cdk4, cdk6, and cdk2 through downregulating cyclin D1 and cyclin D3 complexes. This is accompanied by an upregulation of the tumor suppressor p21 and downregulation of the downstream protooncogene c-MYC [40]. Similarly, in androgen receptorpositive prostate cancer cells, 1,25(OH)2D3 treatment inhibits cdk2 activity and induces G0/G1 arrest [41].
Treatment with 1,25(OH)2D3 can upregulate the expression of the protective gene breast cancer type 1 susceptibility protein (BRCA1) at the mRNA and protein levels in breast cancer cell line MCF-7 but not in the resistant cancer cell line MDA-MB-436. In MCF-7 and other breast cancer cell lines, estrogen receptor (ER) expression is correlated with increased sensitivity to the anti-proliferative effects of 1,25(OH)2D3, indicating that 1,25(OH)2D3 may be particularly effective in ER+ breast cancer cell lines [42].
Treating SCC25 head and neck squamous cell carcinoma cells with 1,25(OH)2D3 can suppress c-MYC, CDK4, and CCND2 expression and increase c-Myc cellular degradation in a vitamin D receptor-dependent manner. This process is associated with an upregulation of expression and stability of MAD1/MXD1, the c-Myc antagonist transcriptional repressor [43].
In colorectal cancers, 1,25(OH)2D3 inhibition of proliferation occurs via JNK1 phosphorylation [44]. In adenoma and carcinoma colorectal cell lines (SW620, PC/JW, and HT29), treating cells with 1,25(OH)2D3 increases the number of cells arrested at G1 phase and the number of apoptotic cells by upregulating the proapoptotic protein Bak in a p53- independent manner [45]. Some p53-dependent apoptotic effects of 1,25(OH)2D3 associated with upregulating the DNA damage repair factor GADD45 can also occur, as shown in human glioma cell lines [46].
Telomerase activity can promote survival of cancer cells by sustaining proliferative growth. Treating human ovarian cancer cell lines with 1,25(OH)2D3 can downregulate telomerase activity by destabilizing and degrading human telomerase reverse transcriptase (hTERT) mRNA of the telomerase enzyme. This process, in turn, promotes apoptotic cell death in ovarian cancer cells. Further studies have shown that treating ovarian cancer cells with 1,25(OH)2D3 blocks telomerase activity through increased expression of microRNA-498 (miRNA-498) [47]. Other miRNAs respond to 1,25(OH)2D3 regulation, supporting a therapeutic inhibitory potential of vitamin D3 in cancer treatment: miRNA-22, miRNA-627, miRNA-181a, miRNA-181b, miRNA-32, and miRNA-98 [48-].

Microenvironment, Oxidative Stress, Inflammatory Signaling, and Angiogenesis

Inflammation is a key hallmark of oncogenesis, contributing to malignant transformation, tumor growth, survival, and metastasis [49]. Oxidative stress and formation of reactive oxygen species and reactive nitrogen species produced by tumor- infiltrating immune cells can damage DNA or modify it epi- genetically at important cell cycle control gene loci that can include tumor suppressor genes [50]. Inflammatory mediators such as interleukins (IL) and cytokines upregulate pathways that activate Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and signal transducer and activator of transcription 3 (STAT3) and restrict the activation of apoptosis needed for cellular control following oxidative stress. Such signaling can activate inhibitor kappa B (IkB) in tumor cells, promoting their sustained survival. Moreover, epithelial to mesenchymal transition promoting metastasis increases in the presence of inflammation in the tumor microenvironment. This process is associated with the increased expression of inflammatory cytokines such as transforming growth factor ß (TGFß) and tumor necrosis factor a (TNFa) and metastatic cell surface markers such as cadherin 11 and fibroblast- specific protein (FSP-1) [51]. The inflammatory involvement of IL-1, IL-6, and IL-8 has been documented in several cancers. IL-8 amplifies tumor-associated inflammation and promotes neovascularization toward tumors, thus promoting growth. IL-6 promotes tumor growth by upregulating the oncogenic K-ras pathway [52]. NF-kB mediates the expression of prostaglandin-endoperoxidase synthase-2/cyclooxygenase 2 (PGHS-2/Cox2), the rate-limiting enzyme involved in converting arachidonic acid to prostaglandin (PGE-2), which potentiates inflammatory response. PGHS-2 furthermore promotes secretion of vascular endothelial growth factor 1 (VEGF1), angiogenesis, and increased endothelial cell permeability to leukocytes and their recruitment to tumor sites, resulting in enhanced oxidative stress and formation of reactive oxygen species [53]. NF-kB activation can upregulate the cell cycle oncogenes CCND1 and c-MYC, induce antiapoptotic changes by upregulating c-FLIP, Survivin, BcL-XL, and upregulating cell adhesion molecules for homing and metastasis namely, ICAM-1, ELAM-1, and VCAM- 17. NF-kB also exacerbates the aggressive invasive nature of tumor cells through upregulated metalloproteinase gene expression and urokinase-type plasminogen activator (uPA) [54, 55]. IL-1 is produced by several cells in the tumor microenvironment, including tissue-specific tumor cells, stromal cells, immune cells infiltrating the tumor site, and endothelial cells. IL-1 can mediate several functions promoting malignancy, including upregulating PGHS-2/Cox2 and angiogenesis [56]. Several studies show that 1,25(OH)2D3 can exert antiinflammatory effects and counteract the effects of many of the preceding proinflammatory interleukins and cytokines as well as downregulate their downstream genes [57].
1,25(OH)2D3 inhibits prostaglandin action by inhibiting the expression of PGHS-2/Cox2, its receptor on cells, and promotes the degradation of the proinflammatory mediator by upregulating gene transcription of 15- hydroxyprostaglandin dehydrogenase (15-PGDH) [58**]. In primary prostatic cell cultures of normal epithelial and adenocarcinoma cells, treating cells with 1,25(OH)2D3 decreases prostatic inflammation by reducing IL-6 expression. This occurs through upregulation by 1,25(OH)2D3 of mitogen- activated protein kinase phosphatase 5 (MKP-5), which inhibits p38 MAPK. MKP-5 dephosphorylates the stress- activated protein kinase p38, which in turn suppresses the downstream transcription of IL-6. 1,25(OH)2D3 also attenuates IL-6 production and overrides carcinogenic challenge of these cells with TNFa or UV stimulation.. This finding highlights the protective and preventive role of 1,25(OH)2D3 in prostate cell lines. However, in metastatic cell lines such as DU145 and PC3, which constitutively express high levels of IL-6, the data indicate that 1,25(OH)2D3 may be effective against external proinflammatory stress and may play a role in prostate cancer prevention in a dose-dependent manner. A defined time window of treatment and use of a higher concentration of 1,25(OH)2D3 (> 50 ng/ml in the treatment medium) may be needed to be effective in metastatic prostate cancer cells [59].
The anti-inflammatory effect of 1,25(OH)2D3 through suppression of interleukin-mediated inflammation and angiogenesis mentioned above has been documented in several in vitro studies. Treating several cancer cell lines with 1,25(OH)2D3 can suppress expression of IL-8, IL-1|3, IL-6, IL-10, IL-17, and TGF|3 and associated proinflammatory downstream signaling [48**, 60, 61, 62**].

Metastasis and Cell Adhesion Molecules

Epithelial to mesenchymal transition (EMT) is an important process of cellular change that initiates tumor cell metastasis. EMT is accompanied by changes in the microenvironment of the tumor and cellular changes within transformed cancer cells. Treatment with vitamin D3 can have several anticancer effects on molecules regulating angiogenesis, invasion, and metastasis in vitro and in vivo [63]. In squamous cell carcinoma cells, 1,25(OH)2D3 upregulates the cell surface adhesion marker E-cadherin, which is important in maintaining epithelial cell-cell junctions and suppressing transition from benign to metastatic malignant lesions. Treatment also downregulates the expression and secretion of matrix metalloproteinases MMP-2 and MMP-9 needed for invasion as documented both in migration assays and in experimentally induced tumor mouse models [64].
Treating glioma C6 cells with 1,25(OH)2D3 downregulates the extracellular matrix protein tenascin-C, thus blocking cell migration of transformed cells, angiogenesis, and metastatic invasion [65]. The dose-dependent anticancer effect of 1,25(OH)2D3 on tenascin-C also has been documented in normal and malignant mouse and human mammary epithelial cell lines [66]. In experiments with breast cancer cells, both the ER+ cell line MCF-7 and ERTcell line MDA-MB-231, treatment with 1,25(OH)2D3 has a very effective dose-dependent ability in blocking cytoskeletal changes, migration, and metastatic invasion. In a dose-dependent manner, 1,25(OH)2D3 upregulates tumor suppressor molecule PDZ-LIM domain- containing protein 2 (PDLIM2) involved in cytoskeletal stability, modulation of focal adhesion, and cell movement and hence suppresses tumorigenicity of cells. In MCF-7 cells, this is accomplished after 12-h treatment with vitamin D3. In MDA-MB-231 cells, this is accomplished by 48 h of treatment, indicating that dose-dependent effects are important when considering treatment with vitamin D3 as well as the presence of different molecular mechanisms that could be targeted in both carcinomas [67].
Recent findings in 143B osteosarcoma cell lines show that vitamin D3 treatment can suppress gene expression of several genes involved in cell invasion and metastasis in a Runx2- mediated fashion. Those genes include genes that support metastasis, such as fibroblast growth factor 1 and 2 (FGF1 and FGF2), bone morphogenic protein 1 (BMP-1), Integrin 34 (ITGB4), MMP-1 and MMP-28, kallikrein-related peptidase- 7 (KLK7), and matrix extracellular phosphoglycoprotein (MEPE) [68]. Similar findings of MMP inhibition have been documented in other cancer cell lines, such as prostate cancer [60, 63].


In the battle against cancer, prevention is as important as cure. Autophagy is a preventive way to protect the body from the accumulation of damaged cells affected by stresses such as oxidative stress. It is a mechanism by which cells of the immune system clear out damaged or worn-out cells that cannot be repaired by DNA excision repair or other mechanisms. 1,25(OH)2D3 can modulate autophagy and increase cellular survival in both healthy tissues and cancer cells. Recent studies have shown that expression of the vitamin D receptor can regulate autophagy in normal breast mammary glands and in MCF-7 luminal epithelial breast cancer cells. This mechanism both has a preventive effect and is useful when using vitamin D3 as an adjuvant during chemotherapy to promote the clearance of chemotherapy-affected cells [69**].

Future Considerations for the Use of Vitamin D3 that Need Further Investigation

Recent studies by Dimitrov and colleagues showed that in human but not mouse tumor cells, treatment with 1,25(OH)2D3 upregulates the mRNA expression of programmed death ligand-1 (PD-L1) in epithelial and myeloid cells. PD-L1, in turn, activates PD-1, thus subduing and suppressing the cytotoxic T cell response of killer T cells needed to activate antitumor immune activity [70]. Such recent initial yet controversial findings compel us to approach the effects of vitamin D3 on the immune system with caution. Because vitamin D3 affects the innate and adaptive immune system phases differently, further studies are needed to address using vitamin D3 in cancer prevention and therapy in a tissue-specific, time-dependent, and dose-dependent manner.
However, several studies have indicated that when used as an adjuvant to chemotherapy, vitamin D3 may be important in potentiating the effects of classical chemotherapeutic agents. Vitamin D3 can act to potentiate chemotherapies by promoting apoptosis, decreasing angiogenesis, and promoting anti-inflammatory palliative effects that help clear chemotherapy- and radiotherapy-sensitized tumor cells [71-74].


Vitamin D3’s role in reducing risk of cancer incidence and death has been increasingly supported in the past few years by the findings of RCTs and observational studies conducted on participants in vitamin D3 supplementation studies. Mechanisms that explain how vitamin D3 does so are well known. For optimal reduction in risk and survival, 25(OH)D3 in the range 40-60 ng/ml is recommended.


Papers of particular interest, published recently, have been highlighted as: •• Of major importance

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