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Beef was more tender and contained more vitamin D when finishing diet included 4,000 IU of Vitamin D – Dec 2017

Cholecalciferol supplementation of heifer diets increases beef vitamin D concentration and improves beef tenderness

Meat Science, Volume 134, December 2017, Pages 103–110, https://doi.org/10.1016/j.meatsci.2017.07.024


Supplementation probably also increased semi-activated vitamin D – which benefits the human, but it rarely tested
Previous studies gave 10X more vitamin D to cattle

Sarah K. Duffya, John V. O'Dohertya, , , Gaurav Rajauriaa, Louise C. Clarkea, Kevin D. Cashmanb, Aoife Hayesb, Michael N. O'Gradyc, Joseph P. Kerryc, Alan K. Kellya
• Dietary biofortification can successfully enhance beef vitamin D content.
• Vitamin D₃ supplementation linearly increases serum 25-OH-D₃ and beef 25-OH-D₃.
• Vitamin D₃ enriched diets decreased Longissimus thoracis shear force values.
• Biofortified beef can contribute 13.5% to daily dietary requirements of vitamin D.

This study investigated the effects of cholecalciferol (vitamin D₃) supplementation on beef vitamin D activity, beef tenderness and sensory attributes. Thirty heifers were randomly allocated to one of three finishing dietary treatments [(T1) basal diet + 0 IU vitamin D₃; (T2) basal diet + 2000 IU vitamin D₃; and (T3) basal diet + 4000 IU vitamin D₃] for a 30 day period pre-slaughter. Vitamin D₃ supplementation linearly increased serum 25-hydroxyvitamin D₃ (25-OH-D3) concentrations (R2 = 0.48), Longissimus thoracis (LT) total vitamin D activity (R2 = 0.78) as well as individually vitamin D₃ (R2 = 0.84) and 25-OH-D₃ (R2 = 0.75). The highest vitamin D₃ inclusion diet (T3) had a 42% increase (P < 0.001) in LT vitamin D activity compared to the intermediate diet (T2) and a 145% increase over the lowest level diet (T1). Vitamin D₃ supplementation decreased LT shear (P < 0.05) force values after 14 days chilling. Sensory parameters were not affected (P > 0.05). In conclusion, through short-term vitamin D₃ supplementation of cattle diets, beef vitamin D activity can successfully be enhanced.

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